بررسی صفات اسپرم رقیق‌شده قوچ با اسانس آویشن (Thymus vulgaris)، با استفاده از میکروسکوپ‌ فاز‌کنتراست و نرم‌افزار CASA

الموضوعات :

حدیثه قمری منور ¹ (دانشجوی دکترای گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
غلامعلی مقدم ² (استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
حسین دقیق کیا ³ (استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)
بابک قاسمی پناهی ⁴ (استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.)

تاريخ الإرسال : 29 الإثنين , رجب, 1444 تاريخ التأكيد : 18 السبت , محرم, 1445 تاريخ الإصدار : 01 الجمعة , شوال, 1444

الکلمات المفتاحية: آویشن, اسپرم, قوچ, صفات حرکتی, نرم‌افزار CASA,

ملخص المقالة :

توانایی اسپرم در انجام لقاح به صفات مختلفی ازجمله حرکت، زنده مانی و حجم کافی بستگی دارد. پژوهش حاضر به‌منظور بررسی تاثیر اسانس آویشن بر صفات حرکتی اسپرم قوچ با استفاده از میکروسکوپ فاز کنتراست و نرم‌افزار CASA (Computer Assisted Sperm Analyzer) انجام گرفت. بدین منظور اسپرم گیری از 4 رأس قوچ و هفته ای دوبار انجام شد. ارزیابی اولیه شامل حجم، حرکت موجی و پیش رونده، حرکت درجا و درصد زنده مانی اسپرم ها بود. سپس نمونه های اسپرم با رقیق کننده شاخص استاندارد بر پایه تریس، 20 درصد زرده تخم مرغ، 7 درصد گلیسرول، به علاوه مقادیر صفر، 100، 200 و 400 میکروگرم اسانس آویشن، مخلوط شدند. بعد از رساندن دمای نمونه های اسپرم به 5 درجه سلسیوس در یخچال، به مدت 10 دقیقه در فاصله 4 سانتی متری بالای نیتروژن مایع قرار گرفتند و در نهایت داخل آن غوطه ور شدند. نمونه ها در روزهای صفر و 30 آزمایش، یخ گشایی شدند. در روزهای انجماد و ذوب، تحرک کل و پیش رونده، حرکت درجا، درصد زنده مانی و صفات CASA و مقدار مالون دی آلدئید (malondialdehyde) مورد آزمایش قرار گرفتند. نتایج نشان داد که درصد زنده مانی و تحرک و مقدار MDA در طول دوره آزمایش کاهش معنی داری داشتند (001/0>p) و تیمارهای 100، 200 و 400 میکروگرم آویشن، بیشترین اختلاف معنی دار را نسبت به گروه شاهد در صفات حرکت پیش رونده و کل، زنده‌مانی و CASA به خود اختصاص دادند (001/0>p). در صفت MDA تیمارهای 100 و 200 اختلاف معنی‌داری نسبت به تیمار 400 و شاهد داشتند (001/0>p). نتایج پژوهش حاضر نشان داد که استفاده از اسانس آویشن به عنوان رقیق‌کننده باعث بهبود صفات حرکتی اسپرم و زنده مانی در طول انجماد می شود.

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