ارزیابی مصرف شیرین‌کننده مصنوعی Cipla بر منحنی الکتروفورتیک پروتئین‌های سرم در موش‌های صحرایی نر دیابتی‌شده
الموضوعات :
حامد نصیری
1
,
جعفر رحمانی کهنموئی
2
1 - دانش‌آموخته دکترای حرفه‌ای، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
تاريخ الإرسال : 09 الإثنين , جمادى الثانية, 1441
تاريخ التأكيد : 25 الإثنين , رمضان, 1441
تاريخ الإصدار : 26 الثلاثاء , جمادى الأولى, 1441
الکلمات المفتاحية:
موش‌صحرایی,
Cipla,
سوکرالوز,
منحنی الکتروفورتیک,
پروتئین‌های سرم,
ملخص المقالة :
سوکرالوز شیرین‌کننده مصنوعی مشتق از ساکارز می‌باشد که 600 بار از شکر شیرین‌تر می‌باشد ولی کالری کمی ایجاد می‌کند. Ciplaبه عنوان یک شیرین‌کننده تجاری علاوه بر سوکرالوز، واجد ترکیبات مختلفی همچون لاکتوز و اِل لوسین نیز می‌باشد. هدف از مطالعه حاضر بررسی تاثیر استفاده از Cipla بر پروتئین‌های سرم در موش های صحرایی سالم و دیابتی شده بود. تعداد 24 سر موش صحرایی نر انتخاب و به صورت تصادفی به 4 گروه مساوی شاهد سالم، شاهد دیابتی، تیمار سالم و تیمار دیابتی شده تقسیم شدند. گروه های شاهد سالم و شاهد دیابتی جیره پایه و گروه های تیمار سالم و تیمار دیابتی شده، روزانه و به‌مدت یک‌ماه Ciplaبا دوز mg/kg 15 از طریق گاواژ دریافت کردند. در طی دوره آزمایش شرایط یکسان محیطی برای همه گروه‌ها اعمال شد. پس از اتمام دوره آزمایش ، از همه موش ها خون گیری انجام گرفته و بعد از جداسازی سرم، با روش الکتروفورز استات سلولز، منحنی الکتروفورز پروتئین‌های سرم تهیه و مورد بررسی قرار گرفت. میزان پروتئین تام و آلبومین سرم در حیوانات گروه های تیمار سالم و شاهد دیابتی به طور غیرمعنی‌داری (05/0p >) بیشتر از میزان آن ها در سرم موش های بقیه گروه‌ها بود ولی میزان آلفا1 گلبولین در سرم موش های گروه شاهد سالم به طور معنی‌داری (05/0> p ) پائین‌تر از میزان آن در سرم حیوانات گروه های شاهد دیابتی و تیمار سالم برآورد شد. همچنین میزان آلفا2 گلبولین و بتاگلبولین به طور غیرمعنی‌داری (05/0p >) در سرم حیوانات گروه های تیمار دیابتی شده و تیمار سالم بالاتر از میزان آن ها در سرم موش های هر دو گروه شاهد سالم و شاهد دیابتی بود. از لحاظ میزان گاماگلبولین سرم نیز اختلاف آماری معنی‌داری در بین گروه های مورد مطالعه وجود نداشت (05/0p >). به نظر می رسد که مصرفCiplaبه غیر از مقدار آلفا1 گلوبولین، تاثیر چندانی بر منحنی الکتروفورتیک پروتئین‌های سرم موش صحرائی ندارد.
المصادر:
Abou-Donia, M. B., El-Masry, E. M., Abdel-Rahman, A. A., McLendon, R. E. and Schiffman, S.S. (2008). Splenda alters gut microflora and increases intestinal p-glycoprotein and cytochrome p-450 in male rats Journal of Toxicology and Environmental Health, 71(21):1415-1429.
Arruda, J., Martins, A.T. and Azoubel, R. (2003). Sodium cyclamate and fetal kidney. Infant, 3(2): 147-150.
Baird, I.M., Shephard, N., Merritt, R. and Hildick-Smith, G. (2000). Repeated dose study of sucralose tolerance in human subjects. Food and Chemical Toxicology, 38(2): 123-129.
Barndt, R. and Jackson, G. (1990). Stability of sucralose in baked goods. Food Technology, 44: 62-66.
Binns, N.M. (2003). Sucralose–all sweetness and light. Nutrition Bulletin, 28(1): 53-58.
Brown, R.J., De Banate, M.A. and Rother, K.I. (2010). Artificial sweeteners: a systematic review of metabolic effects in youth. International Journal of Paediatric Obesity, 5(4): 305-312.
Davidson, T., Sample, C. and Swithers, S. (2014). An application of Pavlovian principles to the problems of obesity and cognitive decline. Neurobiology of Learning and Memory, 108: 172-184.
De Koning, L., Malik, V.S., Rimm, E.B., Willett, W.C. and Hu, F.B. (2011). Sugar-sweetened and artificially sweetened beverage consumption and risk of type 2 diabetes in men. The American Journal of Clinical Nutrition, 93(6): 1321-1327.
De Matos, M.A., Martins, A.T. and Azoubel, R. (2006). Effects of sodium cyclamate on the rat placenta: A morphometric study. International Journal of Morphology, 24(2): 137-142.
Dhingra, R., Sullivan, L., Jacques, P.F., Wang, T.J., Fox, C.S., Meigs, J.B., et al. (2007). Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation, 116(5): 480-488.
Fagherazzi, G., Vilier, A., Sartorelli, D.S., Lajous, M., Balkau, B. and Clavel-Chapelon, F. (2013). Consumption of artificially and sugar-sweetened beverages and incident type 2 diabetes. The American Journal of Clinical Nutrition, 97(3): 517-523.
Fowler, S.P., Williams, K., Resendez, R.G., Hunt, K.J., Hazuda, H.P. and Stern, M.P. (2008). Artificially sweetened beverage use and long‐term weight gain. Obesity, 16(8):1894-1900.
Goldsmith, L.A. and Meckel, C.M. (2000). Alternative Sweeteners. 3rd ed., New York: Marcel Dekker, pp: 185-207.
Grotz, V.L., Henry, R.R., McGill, J.B., Prince, M.J., Shamoon, H., Trout, J.R., et al. (2003). Lack of effect of sucralose on glucose homeostasis in subjects with type 2 diabetes. Journal of the American Dietetic Association, 103(12): 1607-1612.
Hassanpour, A., Alipour, K.H. and Moghaddam, S. (2018). Evaluation of serum concentration of haptoglobin and amyloid A in horses with gorm. Journal of Veterinary Clinical Pathology, 11(3): 277-284. [In Persian]
Jang, H.J., Kokrashvili, Z., Theodorakis, M.J., Carlson, O.D., Kim, B.J., Zhou, J., et al. (2007). Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proceedings of the National Academy of Sciences, 104(38): 15069-15074.
Kyriazis, G.A., Soundarapandian, M.M. and Tyrberg, B. (2012). Sweet taste receptor signalling in beta cells mediates fructose-induced potentiation of glucose-stimulated insulin secretion. Proceedings of the National Academy of Sciences, 109(8): E524-E532.
Lutsey, P.L., Steffen, L.M. and Stevens, J. (2008). Dietary intake and the development of the metabolic syndrome. Circulation, 117(6): 754-761.
Martins, A.T., Azoubel, R., Lopes, R.A., di Matteo, M.A.S. and de Arruda, J.G.F. (2005). Effect of sodium cyclamate on the rat fetal liver. International Journal of Morphology, 23(3): 221-226.
Mezitis, N.H., Maggio, C.A., Koch, P., Quddoos, A., Allison, D.B. and Pi-Sunyer, F.X. (1996). Glycemic effect of a single high oral dose of the novel sweetener sucralose in patients with diabetes. Diabetes Care, 19(9): 1004-1005.
Modaresi, M. (2011). The effect of cinnamon extract on serum proteins levels of male Balb/c mice. Armaghane Danesh, 16(5): 444-452. [In Persian]
Mohajeri, D., Mousavi, Ga. and Mohammadi, P. (2012). Histopathological study of the effect of alcoholic extract of turnip root on ischemia-reperfusion injury in rat. Journal of Veterinary Clinical Pathology, 6(2): 1549-1559. [In Persian]
Nakagawa, Y., Nagasawa, M., Yamada, S., Hara, A., Mogami, H., Nikolaev, V.O., et al. (2009). Sweet taste receptor expressed in pancreatic β-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion. PloS one, 4(4): e5106.
Nasirzadeh, M.R. and Rahmani-Khanmoei, J. (2019). Biochemical effects of alcoholic extract of olive leaf in ovariectomized rats. Journal of Veterinary Clinical Pathology, 12(3): 233-241. [In Persian]
Nehrling, J.K., Kobe, P., McLane, M.P., Olson, R.E., Kamath, S. and Horwitz, D.L. (1985). Aspartame use by persons with diabetes. Diabetes Care, 8(5): 415-417.
Nematollahi, A. and Jafari, R. (2012). Biochemical changes of sheep blood serum in
gastrointestinal nematodes. Journal of Veterinary Clinical Pathology, 6(4): 1697-1701. [In Persian]
Nettleton, J.A., Lutsey, P.L., Wang, Y., Lima, J.A., Michos, E.D. and Jacobs, D.R. (2009). Diet soda intake and risk of incident metabolic syndrome and type 2 diabetes in the Multi-Ethnic Study of Atherosclerosis (MESA). Diabetes Care, 32(4): 688-694.
Palmnäs, M.S., Cowan, T.E., Bomhof, M.R., Su, J., Reimer, R.A., Vogel, H.J., et al. (2014). Low-dose aspartame consumption differentially affects gut microbiota-host metabolic interactions in the diet-induced obese rat. PloS one, 9(10): e109841.
Portela, G. and Azoubel, R. (2004). Toxicity of sucralose in humans. International Journal of Morphology, 26(1): 12-18.
Rahmani-Khanmoei, J. (2016). The effect of inactivated inhaled cigarette smoke on serum lipid profile in rats. Journal of Veterinary Clinical Pathology, 10(3): 245-250. [In Persian]
Rahmani-Khanmoei, J. and Asadi, G. (2016). The Effect of Commercial Sweetener “Cipla” on the Serum Lipid Profiles in Diabetes-Induced Rats. Crescent Journal of Medical and Biological Sciences, 3(4): 136-138.
Rahmani-Khanmoei, J. and Ranjbar, A. (2014). Comparative study of the effect of sucralose and sugar on some serum biomarkers of rat. Indian Journal of Fundamental and Applied Life Sciences, 4(1): 10-15.
Rodero, A.B., Rodero, L.D.S. and Azoubel, R. (2009). Toxicity of sucralose in humans: a review. International Journal of Morphology, 27(1): 239-244.
Shastry, C., Yatheesh, C. and Aswathanarayana, B. (2012). Comparative evaluation of diabetogenic and mutagenic potential of artificial sweeteners-aspartame, acesulfame-K and sucralose. Nitte University Journal of Health Science, 2(3): 80-81.
Suez, J., Korem, T., Zeevi, D., Zilberman-Schapira, G., Thaiss, C.A., Maza, O., et al. (2014). Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature, 514(7521): 181-186.
Swithers, S.E., Martin, A.A. and Davidson, T.L. (2010). High-intensity sweeteners and energy balance. Physiology and Behaviour, 100(1): 55-62.
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Abou-Donia, M. B., El-Masry, E. M., Abdel-Rahman, A. A., McLendon, R. E. and Schiffman, S.S. (2008). Splenda alters gut microflora and increases intestinal p-glycoprotein and cytochrome p-450 in male rats Journal of Toxicology and Environmental Health, 71(21):1415-1429.
Arruda, J., Martins, A.T. and Azoubel, R. (2003). Sodium cyclamate and fetal kidney. Infant, 3(2): 147-150.
Baird, I.M., Shephard, N., Merritt, R. and Hildick-Smith, G. (2000). Repeated dose study of sucralose tolerance in human subjects. Food and Chemical Toxicology, 38(2): 123-129.
Barndt, R. and Jackson, G. (1990). Stability of sucralose in baked goods. Food Technology, 44: 62-66.
Binns, N.M. (2003). Sucralose–all sweetness and light. Nutrition Bulletin, 28(1): 53-58.
Brown, R.J., De Banate, M.A. and Rother, K.I. (2010). Artificial sweeteners: a systematic review of metabolic effects in youth. International Journal of Paediatric Obesity, 5(4): 305-312.
Davidson, T., Sample, C. and Swithers, S. (2014). An application of Pavlovian principles to the problems of obesity and cognitive decline. Neurobiology of Learning and Memory, 108: 172-184.
De Koning, L., Malik, V.S., Rimm, E.B., Willett, W.C. and Hu, F.B. (2011). Sugar-sweetened and artificially sweetened beverage consumption and risk of type 2 diabetes in men. The American Journal of Clinical Nutrition, 93(6): 1321-1327.
De Matos, M.A., Martins, A.T. and Azoubel, R. (2006). Effects of sodium cyclamate on the rat placenta: A morphometric study. International Journal of Morphology, 24(2): 137-142.
Dhingra, R., Sullivan, L., Jacques, P.F., Wang, T.J., Fox, C.S., Meigs, J.B., et al. (2007). Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation, 116(5): 480-488.
Fagherazzi, G., Vilier, A., Sartorelli, D.S., Lajous, M., Balkau, B. and Clavel-Chapelon, F. (2013). Consumption of artificially and sugar-sweetened beverages and incident type 2 diabetes. The American Journal of Clinical Nutrition, 97(3): 517-523.
Fowler, S.P., Williams, K., Resendez, R.G., Hunt, K.J., Hazuda, H.P. and Stern, M.P. (2008). Artificially sweetened beverage use and long‐term weight gain. Obesity, 16(8):1894-1900.
Goldsmith, L.A. and Meckel, C.M. (2000). Alternative Sweeteners. 3rd ed., New York: Marcel Dekker, pp: 185-207.
Grotz, V.L., Henry, R.R., McGill, J.B., Prince, M.J., Shamoon, H., Trout, J.R., et al. (2003). Lack of effect of sucralose on glucose homeostasis in subjects with type 2 diabetes. Journal of the American Dietetic Association, 103(12): 1607-1612.
Hassanpour, A., Alipour, K.H. and Moghaddam, S. (2018). Evaluation of serum concentration of haptoglobin and amyloid A in horses with gorm. Journal of Veterinary Clinical Pathology, 11(3): 277-284. [In Persian]
Jang, H.J., Kokrashvili, Z., Theodorakis, M.J., Carlson, O.D., Kim, B.J., Zhou, J., et al. (2007). Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proceedings of the National Academy of Sciences, 104(38): 15069-15074.
Kyriazis, G.A., Soundarapandian, M.M. and Tyrberg, B. (2012). Sweet taste receptor signalling in beta cells mediates fructose-induced potentiation of glucose-stimulated insulin secretion. Proceedings of the National Academy of Sciences, 109(8): E524-E532.
Lutsey, P.L., Steffen, L.M. and Stevens, J. (2008). Dietary intake and the development of the metabolic syndrome. Circulation, 117(6): 754-761.
Martins, A.T., Azoubel, R., Lopes, R.A., di Matteo, M.A.S. and de Arruda, J.G.F. (2005). Effect of sodium cyclamate on the rat fetal liver. International Journal of Morphology, 23(3): 221-226.
Mezitis, N.H., Maggio, C.A., Koch, P., Quddoos, A., Allison, D.B. and Pi-Sunyer, F.X. (1996). Glycemic effect of a single high oral dose of the novel sweetener sucralose in patients with diabetes. Diabetes Care, 19(9): 1004-1005.
Modaresi, M. (2011). The effect of cinnamon extract on serum proteins levels of male Balb/c mice. Armaghane Danesh, 16(5): 444-452. [In Persian]
Mohajeri, D., Mousavi, Ga. and Mohammadi, P. (2012). Histopathological study of the effect of alcoholic extract of turnip root on ischemia-reperfusion injury in rat. Journal of Veterinary Clinical Pathology, 6(2): 1549-1559. [In Persian]
Nakagawa, Y., Nagasawa, M., Yamada, S., Hara, A., Mogami, H., Nikolaev, V.O., et al. (2009). Sweet taste receptor expressed in pancreatic β-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion. PloS one, 4(4): e5106.
Nasirzadeh, M.R. and Rahmani-Khanmoei, J. (2019). Biochemical effects of alcoholic extract of olive leaf in ovariectomized rats. Journal of Veterinary Clinical Pathology, 12(3): 233-241. [In Persian]
Nehrling, J.K., Kobe, P., McLane, M.P., Olson, R.E., Kamath, S. and Horwitz, D.L. (1985). Aspartame use by persons with diabetes. Diabetes Care, 8(5): 415-417.
Nematollahi, A. and Jafari, R. (2012). Biochemical changes of sheep blood serum in
gastrointestinal nematodes. Journal of Veterinary Clinical Pathology, 6(4): 1697-1701. [In Persian]
Nettleton, J.A., Lutsey, P.L., Wang, Y., Lima, J.A., Michos, E.D. and Jacobs, D.R. (2009). Diet soda intake and risk of incident metabolic syndrome and type 2 diabetes in the Multi-Ethnic Study of Atherosclerosis (MESA). Diabetes Care, 32(4): 688-694.
Palmnäs, M.S., Cowan, T.E., Bomhof, M.R., Su, J., Reimer, R.A., Vogel, H.J., et al. (2014). Low-dose aspartame consumption differentially affects gut microbiota-host metabolic interactions in the diet-induced obese rat. PloS one, 9(10): e109841.
Portela, G. and Azoubel, R. (2004). Toxicity of sucralose in humans. International Journal of Morphology, 26(1): 12-18.
Rahmani-Khanmoei, J. (2016). The effect of inactivated inhaled cigarette smoke on serum lipid profile in rats. Journal of Veterinary Clinical Pathology, 10(3): 245-250. [In Persian]
Rahmani-Khanmoei, J. and Asadi, G. (2016). The Effect of Commercial Sweetener “Cipla” on the Serum Lipid Profiles in Diabetes-Induced Rats. Crescent Journal of Medical and Biological Sciences, 3(4): 136-138.
Rahmani-Khanmoei, J. and Ranjbar, A. (2014). Comparative study of the effect of sucralose and sugar on some serum biomarkers of rat. Indian Journal of Fundamental and Applied Life Sciences, 4(1): 10-15.
Rodero, A.B., Rodero, L.D.S. and Azoubel, R. (2009). Toxicity of sucralose in humans: a review. International Journal of Morphology, 27(1): 239-244.
Shastry, C., Yatheesh, C. and Aswathanarayana, B. (2012). Comparative evaluation of diabetogenic and mutagenic potential of artificial sweeteners-aspartame, acesulfame-K and sucralose. Nitte University Journal of Health Science, 2(3): 80-81.
Suez, J., Korem, T., Zeevi, D., Zilberman-Schapira, G., Thaiss, C.A., Maza, O., et al. (2014). Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature, 514(7521): 181-186.
Swithers, S.E., Martin, A.A. and Davidson, T.L. (2010). High-intensity sweeteners and energy balance. Physiology and Behaviour, 100(1): 55-62.
