ارزیابی غلظت سرمی هاپتوگلوبین و آمیلوئید A سرمی در اسب‌های مبتلا به گورم
الموضوعات :
علی حسن پور
1
,
حمیدرضا علیپور خیرخواه
2
,
سینا مقدم
3
1 - دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
3 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.
تاريخ الإرسال : 14 الإثنين , جمادى الثانية, 1438
تاريخ التأكيد : 26 الأربعاء , صفر, 1439
تاريخ الإصدار : 04 الأربعاء , ربيع الأول, 1439
الکلمات المفتاحية:
گورم,
اسب,
پروتئین‌های فاز حاد,
ملخص المقالة :
این مطالعه روی 29 رأس اسب مبتلا به گورم و 27 رأس اسب سالم (کشت منفی) در اسب داری های اطراف تبریز به منظور بررسی سطوح سرمی هاپتوگلوبین و آمیلوئیدA سرمی انجام پذیرفت. بیماری در اسب های مبتلا به گورم بر اساس نشانه های آزمایشگاهی و بالینی تأیید شد. بعد از تأیید آزمایشگاهی از طریق کشت نمونه ها، از دام مربوطه نمونه خون از ورید وداج اخذ و پس از جداسازی سرم، مقادیر پروتئین های فاز حاد (هاپتوگلوبین و آمیلوئید A سرمی) اندازه گیری شد. میانگین میزان آمیلوئید A سرمی در اسب‌های گروه بیمار mg/dl 95/569±13/2749 و در گروه شاهدmg/dl 76/357±97/1602 بود. میانگین سطح سرمی هاپتوگلوبین در اسب‌های مبتلاmg/dl 01/244±20/1258 و در گروه شاهد mg/dl 18/187±97/721 بود. میانگین هر دو پارامتر در اسب‌های گروه بیمار به طور معنی داری بیشتر از گروه سالم بود (05/0>p). در گروه بیمار در بررسی همبستگی بین پارامترهای سرمی اندازه گیری شده ، مشخص گردید که ارتباط معنی داری بین آنها وجود ندارد (269/0r= و 05/0<p). نتیجه نهایی این که بیماری گورم در اسب ها باعث افزایش سطوح سرمی پروتئین های فاز حاد می شود و بنابراین در درمان اسب های مبتلا به گورم این موضوع باید لحاظ گردد.
المصادر:
Boyle, A.G., Sweeney, C.R. and Kristula, M. (2009). Factors associated with likelihood of horses having a high serum Streptococcus equi SeM-specific antibody titer. Journal of American Veterinary Medicine Association, 235(8): 973-977.
Browning, L.M., Krebs, J.D. and Jebb, S.A. (2004). Discrimination ratio analysis of inflammatory markers: implications for the study of inflammation in chronic disease. Metabolism, 53(7):899-903.
Carter, J.N., Meredith, G.L., Montelongo, M., Gill, D.R., Krehbiel, C.R., Payton, M.E., et al. (2002). Relationship of vitamin E supplementation and antimicrobial treatment with acute-phase protein responses in cattle affected by naturally acquired respiratory tract disease. American Journal of Veterinary Research, 63(8): 1111-1117.
Chavan, M., Kawle, P.D. and Mehta, N.G. (2005). Increased sialylation and defucosylation of plasma proteins are early events in the acute phase response. Glycobiology, 15(3): 838-848.
Chavatte, P.M., Pepys, M.B. and Roberts, B. (1992). Measurement of serum amyloid A concentration as an aid to differential diagnosis of infection in newborn foals. Equine Infections Disease, VI: 33-38.
Cohen, N.D., Chaffin, M.K., Vandenplas, M., Edwards, R.E., Nevill, M. and Martens, R.J. (2005). Study of serum amyloid A (SAA) concentrations as a means of achieving early diagnosis of Rhodococcus equi pneumonia in foals. Equine Veterinary Journal, 37(3): 212-216.
Davidson, A., Traub-Dargatz, J.L. and Magnuson, R. (2008). Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles. Journal of Veterinary Diagnosis Investigation, 20(4): 457-462.
Eckersal, P.D. (2000). Recent advances and future prospects for the use of acute phase proteins and markers of disease in animals. Revue de Medecine Veterinaire, 151(7): 577-584.
Fagliari, J.J., McClenahan, D., Evanson, O.A. and Weiss, D.J. (1998). Changes in plasma protein concentrations in ponies with experimentally induced alimentary laminitis. American Journal of Veterinary Research, 59(10): 1234-1237.
Goodall, J. and Kent, J.E. (1991). Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations. Equine Veterinary Journal, 23(1): 59-66.
Guss, B., Flock, M. and Frykberg, L. (2009). Getting to grips with strangles: an effective multicomponent recombinant vaccine for the protection of horses from Streptococcus equi infection. PLoS Pathogens, 5(9):e1000584.
Hultén, C. and Demmers, S. (2002). Serum Amyloid A (SAA) as an Aid in the Management of Infectious Disease in the Foal: Comparison with Total Leucocyte Count, Neutrophil Count and Fibrinogen. Equine Veterinary Journal, 34(7): 693-698.
Hultén, C., Gronlund, U., Hirvonen, J., Tulamo, R.M., Suominen, M.M., Marhaug, G., et al. (2002). Dynamics in serum of the inflammatory markers serum Amyloid A (SAA), haptoglobin, Fibrinogen and alpha 2- globulins during induced noninfectious arthritis in the horse. Equine Veterinary Journal, 34(7): 699-704.
Hultén, C., Sandgren, B., Skioldebrand, E., Klingeborn, B., Marhaug, G. and Forsberg, M. (1999). The acute phase protein serum amyloid A (SAA) as an inflammatory marker in equine influenza virus infection. Acta Veterinaria Scandinavica, 40(4): 323-323.
Jacobsen, S., Thomsen, M.H. and Nanni, S. (2006). Concentrations of Serum Amyloid A in Serum and Synovial Fluid from Healthy Horses and Horses with Joint Disease. American Journal of Veterinary Research, 67(10): 1738-1742.
Milne, E.M., Doxey, D.L. and Kent, J.E. (1991). Clinical and biochemical features of grass sickness (equine dysautonomia). Equine Veterinary Journal, 23(5): 360-364.
Newton, J.R., Verheyen, K. and Talbot, N.C. (2000). Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and cultures of Streptococcus equi. Equine Veterinary Journal, 32(6): 515-516.
Pellegrini Masini, A., Tedeschi, D. and Baragli, P. (2003). Exercise-induced intravascular haemolysis in standardbred horses. Equine Veterinary Journal, 12(4): 45-48.
Petersen, H.H., Nielsen, J.P. and Heegaard, P.M. (2004). Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research, 35(2): 163-187.
Pihl, T.H., Andersen, P., Kjelgaard-Hansen, M., Morck, N. and Jacobsen, S. (2013). Serum amyloid A and haptoglobin concentrations in serum and peritoneal fluid of healthy horses and horses with acute abdominal pain. Veterinary Clinical Pathology, 42(2): 177-183.
Pollock, P.J., Prendergast, M., Schumacher, J. and Bellenger, C.R. (2005). Effects of surgery on the acute response in clinically normal and diseased horses. Veterinary Record, 156(17): 538-542.
Skinner, J.G., Brown, R. and Roberts, L. (1991). Bovine haptoglobin response in clinically defined field conditions. Veterinary Record, 128(7): 147-149.
Skinner, J.G. and Roberts, L. (1994). Haptoglobin as an indicator of infection in sheep. Veterinary Record, 134(4): 33-36.
Stephen, M., Reed, B., Warwick, M., Bayly, D.C. and Sellon, S. (2004). Equine Internal Medicine. 2nd ed., Elsevier, pp: 429-432.
Stoneham, S.J., Palmer, L., Cash, R. and Rossdale, P.D. (2001). Measurement of serum amyloid A in the neonatal foal using a latex agglutination immunoturbidimetric assay: determination of the normal range, variation with age and response to disease. Equine Veterinary Journal, 33(6): 599-603.
Sweeney, C.R., Timoney, J.F. and Newton, R. (2005). Streptococcus equi infections in horses: guidelines for treatment, control and prevention of strangles. Journal of Veterinary Internal Medicine, 19(1): 123-134.
Timoney, J.F. and Kumar, P. (2008). Early pathogenesis of equine Streptococcus equi infection (strangles). Equine Veterinary Journal, 40(7): 637-640.
Uchida, E., Katoh, N. and Takahashi, K. (1993). Appearance of haptoglobin in serum from cows at parturition. Journal of Veterinary Medicine Science, 55(5): 893-894.
Vandenplas, M.L., Moore, J.N. and Barton, M.H. (2005). Concentrations of serum amyloid A and lipopoly saccharide-binding in horses with colic. Animal Journal of Veterinary Research, 66(9): 1509-1516
Weese, J.S., Jarlot, C. and Morley, P.S. (2009): Survival of Streptococus equi on surfaces in an outdoor environment. Canadian Veterinary Journal, 50(9): 968-970.
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Boyle, A.G., Sweeney, C.R. and Kristula, M. (2009). Factors associated with likelihood of horses having a high serum Streptococcus equi SeM-specific antibody titer. Journal of American Veterinary Medicine Association, 235(8): 973-977.
Browning, L.M., Krebs, J.D. and Jebb, S.A. (2004). Discrimination ratio analysis of inflammatory markers: implications for the study of inflammation in chronic disease. Metabolism, 53(7):899-903.
Carter, J.N., Meredith, G.L., Montelongo, M., Gill, D.R., Krehbiel, C.R., Payton, M.E., et al. (2002). Relationship of vitamin E supplementation and antimicrobial treatment with acute-phase protein responses in cattle affected by naturally acquired respiratory tract disease. American Journal of Veterinary Research, 63(8): 1111-1117.
Chavan, M., Kawle, P.D. and Mehta, N.G. (2005). Increased sialylation and defucosylation of plasma proteins are early events in the acute phase response. Glycobiology, 15(3): 838-848.
Chavatte, P.M., Pepys, M.B. and Roberts, B. (1992). Measurement of serum amyloid A concentration as an aid to differential diagnosis of infection in newborn foals. Equine Infections Disease, VI: 33-38.
Cohen, N.D., Chaffin, M.K., Vandenplas, M., Edwards, R.E., Nevill, M. and Martens, R.J. (2005). Study of serum amyloid A (SAA) concentrations as a means of achieving early diagnosis of Rhodococcus equi pneumonia in foals. Equine Veterinary Journal, 37(3): 212-216.
Davidson, A., Traub-Dargatz, J.L. and Magnuson, R. (2008). Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles. Journal of Veterinary Diagnosis Investigation, 20(4): 457-462.
Eckersal, P.D. (2000). Recent advances and future prospects for the use of acute phase proteins and markers of disease in animals. Revue de Medecine Veterinaire, 151(7): 577-584.
Fagliari, J.J., McClenahan, D., Evanson, O.A. and Weiss, D.J. (1998). Changes in plasma protein concentrations in ponies with experimentally induced alimentary laminitis. American Journal of Veterinary Research, 59(10): 1234-1237.
Goodall, J. and Kent, J.E. (1991). Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations. Equine Veterinary Journal, 23(1): 59-66.
Guss, B., Flock, M. and Frykberg, L. (2009). Getting to grips with strangles: an effective multicomponent recombinant vaccine for the protection of horses from Streptococcus equi infection. PLoS Pathogens, 5(9):e1000584.
Hultén, C. and Demmers, S. (2002). Serum Amyloid A (SAA) as an Aid in the Management of Infectious Disease in the Foal: Comparison with Total Leucocyte Count, Neutrophil Count and Fibrinogen. Equine Veterinary Journal, 34(7): 693-698.
Hultén, C., Gronlund, U., Hirvonen, J., Tulamo, R.M., Suominen, M.M., Marhaug, G., et al. (2002). Dynamics in serum of the inflammatory markers serum Amyloid A (SAA), haptoglobin, Fibrinogen and alpha 2- globulins during induced noninfectious arthritis in the horse. Equine Veterinary Journal, 34(7): 699-704.
Hultén, C., Sandgren, B., Skioldebrand, E., Klingeborn, B., Marhaug, G. and Forsberg, M. (1999). The acute phase protein serum amyloid A (SAA) as an inflammatory marker in equine influenza virus infection. Acta Veterinaria Scandinavica, 40(4): 323-323.
Jacobsen, S., Thomsen, M.H. and Nanni, S. (2006). Concentrations of Serum Amyloid A in Serum and Synovial Fluid from Healthy Horses and Horses with Joint Disease. American Journal of Veterinary Research, 67(10): 1738-1742.
Milne, E.M., Doxey, D.L. and Kent, J.E. (1991). Clinical and biochemical features of grass sickness (equine dysautonomia). Equine Veterinary Journal, 23(5): 360-364.
Newton, J.R., Verheyen, K. and Talbot, N.C. (2000). Control of strangles outbreaks by isolation of guttural pouch carriers identified using PCR and cultures of Streptococcus equi. Equine Veterinary Journal, 32(6): 515-516.
Pellegrini Masini, A., Tedeschi, D. and Baragli, P. (2003). Exercise-induced intravascular haemolysis in standardbred horses. Equine Veterinary Journal, 12(4): 45-48.
Petersen, H.H., Nielsen, J.P. and Heegaard, P.M. (2004). Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research, 35(2): 163-187.
Pihl, T.H., Andersen, P., Kjelgaard-Hansen, M., Morck, N. and Jacobsen, S. (2013). Serum amyloid A and haptoglobin concentrations in serum and peritoneal fluid of healthy horses and horses with acute abdominal pain. Veterinary Clinical Pathology, 42(2): 177-183.
Pollock, P.J., Prendergast, M., Schumacher, J. and Bellenger, C.R. (2005). Effects of surgery on the acute response in clinically normal and diseased horses. Veterinary Record, 156(17): 538-542.
Skinner, J.G., Brown, R. and Roberts, L. (1991). Bovine haptoglobin response in clinically defined field conditions. Veterinary Record, 128(7): 147-149.
Skinner, J.G. and Roberts, L. (1994). Haptoglobin as an indicator of infection in sheep. Veterinary Record, 134(4): 33-36.
Stephen, M., Reed, B., Warwick, M., Bayly, D.C. and Sellon, S. (2004). Equine Internal Medicine. 2nd ed., Elsevier, pp: 429-432.
Stoneham, S.J., Palmer, L., Cash, R. and Rossdale, P.D. (2001). Measurement of serum amyloid A in the neonatal foal using a latex agglutination immunoturbidimetric assay: determination of the normal range, variation with age and response to disease. Equine Veterinary Journal, 33(6): 599-603.
Sweeney, C.R., Timoney, J.F. and Newton, R. (2005). Streptococcus equi infections in horses: guidelines for treatment, control and prevention of strangles. Journal of Veterinary Internal Medicine, 19(1): 123-134.
Timoney, J.F. and Kumar, P. (2008). Early pathogenesis of equine Streptococcus equi infection (strangles). Equine Veterinary Journal, 40(7): 637-640.
Uchida, E., Katoh, N. and Takahashi, K. (1993). Appearance of haptoglobin in serum from cows at parturition. Journal of Veterinary Medicine Science, 55(5): 893-894.
Vandenplas, M.L., Moore, J.N. and Barton, M.H. (2005). Concentrations of serum amyloid A and lipopoly saccharide-binding in horses with colic. Animal Journal of Veterinary Research, 66(9): 1509-1516
Weese, J.S., Jarlot, C. and Morley, P.S. (2009): Survival of Streptococus equi on surfaces in an outdoor environment. Canadian Veterinary Journal, 50(9): 968-970.
