Cymbopogon citratus Stapf (DC) extract attenuates gasoline vapour-induced low-triiodothyronine syndrome, oxidative stress and lipid peroxidation in rats

الموضوعات :

Christopher Edet Ekpenyong ¹ ( Department of Physiology, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria )
Utibe Essiet ² ( Department of Physiology, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Nigeria )

تاريخ الإرسال : 22 السبت , شعبان, 1440 تاريخ التأكيد : 23 السبت , ذو الحجة, 1440 تاريخ الإصدار : 02 الأحد , محرم, 1441

الکلمات المفتاحية: Oxidative stress, Rat, Lemongrass diet, petroleum fume, thyroid toxicity,

ملخص المقالة :

Cymbopogon citratus Stapf (DC) (Lemongrass) is a widely distributed aromatic perennial plant that has the potential to mitigate xenobiotic-induced systemic disorders. However, whether C. citratus has any ameliorative effect on gasoline vapour (GV)-induced thyroid gland dysfunction has not been previously evaluated. Therefore, the present study aimed to assess the effect of C. citratus leaf decoctions on GV-induced thyroid gland disorders. Thirty-five Albino rats were segregated into 5 groups (n=7 per group). Animals in group 1 served as unexposed control, while animals in group 2 were exposed to GV alone for 4 weeks. Animals in groups 3, 4 and 5 in addition to being exposed to GV for 4 weeks, were treated with different concentrations of C. citratus leaf extracts for 2 weeks. Animals exposed to GV alone had significant decrease in serum levels of catalase, while serum levels of malondialdehyde significantly (p <0.05) increased. Serum levels of thyroid stimulating hormone significantly (p <0.05) increased and decreased in male and female rats respectively exposed to GV alone. Serum levels of triiodothyronine decreased in both male and female rats exposed to GV alone, whereas serum levels of thyroxine decreased only in female rats. In addition, exposure to GV alone caused significant alterations in the normal histo-morphology of the thyroid gland. Co-administration of C. citratus leaf decoctions caused reversal of these changes, as well. This study showed that C. citratus leaf extract has the potential to attenuate GV-induced thyroid gland disorders and oxidative stress due to its natural bioactive constituents.

المصادر:

Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., Lightfoot, D.A., 2017. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plant 6(4), 42.

Arhoghro, E.M., Kpomah, E.D., 2013. Cymbopogon citratus aqueous extract alleviates cisplatin-induced renal oxidative stress and toxicity in albino rats. Am. J. Res. Comin. 2325-4076.

Azari, M.R., Konjin, Z.I., Zayeri, F., Salehpours, S., Seyedi, M.D., 2012. Occupational exposure of petroleum depot workers to BETEX compound. Int. J. Occup. Environ. Med. 3, 39-44.

Bachiega, T.F., Sforcin, J.M., 2011. Lemongrass and citral effect on cytokines production by murine macrophage. J. Ethnopharmacol. 137(1), 909-913.

Balakrishnan, A., Priya, V., Ciayathri, R., 2015. Preliminary phytochemical analysis and antioxidant activities of lemongrass and lavender. J. Pharmacol. Sci. Res. 7(7), 448-450.

Bao, X.L., Yuan, H.H., Wang, C.Z., Fan, W., Lan, M.B., 2015. Polysaccharides from Cymbopogon citratus with antitumor and immunomodulatory activity. Pharm. Biol. 53(1), 117-24.

Barp, J., Araujo, A.S., Fernandes, T.R., 2002. Myocardial antioxidant and oxidative stress changes due to sex hormones. Braz. J. Med. Biol. Res. 35, 1075-81.

Bharti, S.K., Krishnan, S., Kumar, A., Rajak, K.K., Murari, K., Bharti, B.K., Gupta, A.K., 2012. Antihyperglycemic activity with DPP-IV inhibition of alkaloids from seed extract of Castanospermum australe: Investigation by experimental validation and molecular docking. Phytomedicine 20, 24-31.

Cachofeiro, V., Golcochea, M., de Vinuesa, S.G., Oubina, P., Lahera, V., Luno, J., 2008. Oxidative stress and inflammation a link between chronic kidney disease and cardiovascular disease. Kidney Int. Supplement. 74(111), 4-9.

Cheel, J., Theoduloz, C., Rodriguez, J., Schmeda, H.G., 2005. Free radical and antioxidants (Cymbopogon citratus (DC) Staff.). J. Agric. Food Chem. 53(7), 2511-2517.

Chen, Y., Ji, L.L., Liu, T.Y., Wang, Z.T., 2011. Evaluation of gender-related differences in various oxidative stress enzymes in mice. Chin. J. Physiol. 54(6), 385-390.

Chen, M., Long, Z., Wang, Y., IU, J., Pian, H., Wang, L., Chen, Z., 2013. Protective effect of saponin on a hypertension target organ in spontaneously hypertensive rats. Exp. Ther. Med. 5, 429-432.

Diaz, P., Jeong, S.C., Lee, S., Khoo, C., Koyyalamudim, S.R., 2012. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chin. Med. 7(1), 26.

Draper, H.H., Hadley, M., 1990. Malondialdehyde determination as index of lipid peroxidation Methods Enzym. 186, 421-431.

Ekpenyong, C.E., 2016. Transient hyperbilirubinaemia and sustained elevation of alkaline phosphatase after the ingestion of Lemongrass tea. Elixir Physiol. Anat. 100, 43512-43516.

Ekpenyong, C.E., 2017a. Lemongrass leaf extract attenuates hydrocarbon-induced oxidative stress and macrocytic hypochromic anemia in rats. Int. J. Ecotoxicol. Ecobiol. 2(2), 74-79.

Ekpenyong, C.E., 2017b. Effect of Cymbopogon citratus decoctions on gasoline-vapour-induced reproductive toxicity in female rats. Braz. J. Pharmacol. 15(6), 1-8.

Ekpenyong, C.E., Akpan, E.E., 2015. Use of Cymbopogon citratus essential oil in food preservation: Recent advances and future perspectives. Crit. Rev. Food Sci. Nutr. 57(12), 2541-2559.

Ekpenyong, C.E., Akpan, N.S., 2017. Ameliorative potential of Cymbopogon citratus decoctions on gasoline vapour-induced nephrotoxicity. Nat. Prod. J. 7(1), 37-46.

Ekpenyong, C.E., Asuquo, E.A., 2017. Recent advances in occupational and environmental health hazards of workers exposed to gasoline compounds. Int. J. Occup. Med. Environ. Health 30(1), 1-26.

Ekpenyong, C.E., Bassey, G., 2016.Investigation of the effect of Cymbopogon citratus decoctions on gasoline vapour-induced lipid peroxidation and hepatotoxicity in rats. J. Int. Res. Med. Pharm. Sci. 10(4), 171-181.

Ekpenyong, C.E., Daniel, N.E., Antai, A.B., 2015. Bioactive natural constituents from Lemongrass tea and erythropoiesis boosting effects: Potential use in prevention and treatment of anemia. J. Med. Food 18(1), 118-127.

Ekpenyong, C.E., Davies, K., Antai, E.E., 2014. Cymbopoogn citratus Stapf (DC) extract ameliorates atherogenic cardiovascular risk in diabetes-induced dyslipidemia in rats. J. Adv. Med. Med. Res. 4(28), 4695-4709.

Ekpenyong, C.E.,Osim, E.E., 2016.Changes in blood pressure indices in normotensive adults after the consumption of Lemongrass tea. J. Coast. Life Med. 4(10), 930-936.

Ekpenyong, C.E., Oyebadejo, S.A., 2016. Cymbopogon citratus Stapf (DC) extracts alleviate gasoline vapour-induced metabolic disorders and cardiovascular disease risk in rats. Saudi J. Med. Pharm. Sci. 2(7), 160-169.

Francisco, V., Costa, G., Figueirinha, A., Margues, C., Pereira, P., Miguel, N.B., Celeste, L.M., Ciarcia-Rodriguez, C., Teresa, B.M., 2013. Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-kappa B pathway inhibition: contribution of chlorogenic acid. J. Ethnopharmacol. 148(1), 126-134.

Gayathri, K., Jayachandran, K.S., Vasanthi, H.R., Rajamanickan, G.V., 2011. Cardioprotective effect of lemongrass as evidenced by biochemical and histopathological changes in experimentally induced cardiotoxicity. Human Exp. Toxicol. 30, 1073-1082.

Gelatti, G.T., Horn, R.C., Mori, N.C., Berlezi, E.M., Tissiani, A.C., Mayer, M.S., Gewehr, D.M., 2016. Effect of Cymbopogon citratus on oxidative stress markers in erythrocytes from post menopausal women: A pilot study. J. Plant Studies 5, 20-27.

Hajir, B., Abdllha, A.I., Mohamed, K.A., Almoniem, N.I., Adam, W., Alhaadi, A.A., Elshikh, A.J., Ali, I.G., Makuar, A.M., Elnazeer, N.A., Elrofaei, S.F., Abdoelftah, M.N.H., 2016. Evolution of antimicrobial, antioxidant potentials and phytochemical studies of three solvent extracts of five species from acacia used in Sudanese. Ethnomedicine. Advan. Microbiol. 6, 691-698.

Hiwatashi, K., Shirakawa, H., Hori, K., Yoshiki, Y., Suzuki, N., Hokari, M., Komai, M., Takahashi, S., 2010. Reduction of blood pressure by soya bean saponins, rennin inhibitors from soya bean, in spontaneously hypertensive rats. Biosci. Biotechnol. Biochem. 74(11), 2310-2312.

Hosch, M., Muser, J., Hulter, H.N., Krapf, R., 2003. Ureagenesis: Evidence for a lack of hepatic regulation of acid-base equilibrium in humans. Am. J. Physiol. Renal Physiol. 286, 94-99.

Hussein, R.A., El-Anssary, A.A., 2018. Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants. Intech. Open 2018.

Kander, M.C., Cui, Y., Liu, Z., 2017. Gender difference in oxidative stress: a new look at the mechanisms for cardiovascular disease. J. Cell. Mol. Med. 5, 1024-1032.

Katsukawa, M., Nakata, R., Takizawa, Y., Hon, K., Takahashi, S., Inoue, H., 2010. Citral, A compound of Lemongrass oil, activates PPARα and y and suppresses COX-2 expression. Biochem. Biophys. 180(11), 1214-1220.

Koh, H., Mokhtar, R.A., Igbal, M., 2012. Antioxidant potential of Cymbopogon citratus extract: Alleviation of carbon tetrachloride-induced hepatic oxidative stress and toxicity. Hum. Exp. Toxicol. 31, 81-91.

Liu, D.D., Chen, C., Li, R.W., 2011. Protective effect of flavonoids from Pericarpium citri reticulatae (chenpi) against oxidative stress induced by exhaustive exercise. Afr. J. Microbiol. Res. 5, 50-56.

Lorkes, D., 1983. A new approach to practical acute toxicity testing. Arch. Toxicol. 53, 275-287.

Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.

Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities - A. Ind. Crops Prod. 129, 350-394.

Ogbera, A.O., Fasanmade, O., Adediran, O., 2007. Pattern of thyroid disorder in the southern region of Nigeria. Ethn. Dis. 17, 327-330.

Ogbera, A.O., Kuku, S.F., 2015. Epidemiology of thyroid disease in Africa. Indian J. Endocrinol. Metab. 15(12), S82-S88.

Pongprayoon, U., Soontornsaratune, P., Jarikasem, S., Sematong, T., Wasuwat, S., Claeson, P. 1997. Tropical anti-inflammatory activity of the major lipophilic constituents of the rhizome of Zingiber cassumunar. Part 1: The essential oil. Phytomedicine 3, 319-322.

Praditvarn, L., Samhandharaksa, C., 1990. A study of the volatile oil from stem lemon grass. J. Pharm. Assess. Siam. 32, 87-92.

Rahim, S.M., Taha, E.M., Mubark, Z.M., Aziz, S.S., Simon, K.D., Mazlan, A.G., 2014.  Protective effect of Cymbopogon citratus on hydrogen peroxide-induced oxidative stress in the reproductive system of male rats. Syst. Biol. Reprod. Med. 59(6), 329-336.

Said, A.M., Atwa, S.A., Khalifa, O.A., 2019. Ameliorating effect of gum Arabic and Lemongrass on chronic kidney disease induced experimentally in rats. Bull. Natl. Res. Cent. 43(1), 47.

Salamu, B.A., Odusan, O., Ebili, H.O., Akintola, P.A., 2016. Spectrum and prevalence of thyroid disease seen at a tertiary health facility in Sagamu, South-West Nigeria. Nigerian Postgraduate Med. J. 23, 137-140.

Saleh, M., Rahim, E.M., Taha, Z.M., Mubark, S.S., Aziz, K.D., Simon, A.G.M., 2013. Protective effect of Cymbopogon citratus on hydrogen peroxide-induced oxidative stress in the reproductive system of rats. J. Syst. Biol. Reprod. Med. 59(6), 329-336.

Sari, D.M., Lestarisa, T., Alexandra, F.D., Felita, H., Thalib, I., 2017. Antioxidant and anti-glycation activity of ethanol lemongrass (Cymbopogon citratus) leaves extracts. Int. J. Pharmacol. Phytochem. Res. 9(5), 710-715.

Sarker, S.D., Nahar, L., 2018. Evidence-based phytotherapy: what, why and how? Trends Phytochem. Res. 2(3), 125-126.

Sieniawska, E., Baj, T., Los, R., Skalicka-Wozniak, K., Malm, A., Glowniak, K., 2013. Phenolic acids content, antioxidant and antimicrobial activity of Ligusticum mutellina L. Nat. Prod. Res. 27, 1108-1110.

Singh, B., Chandran, V., Bandhu, H.K., Mittal, B.R., Bhattacharya, A., 2000. Impact of lead exposure on pituitary-thyroid axis in humans. Bio Metals 13(2), 187-192.

Trease, E., Evans, C., 1989. A Textbook of Pharmacognosy. 13th Ed., Baluire, Tindali, London pp. 100-101.

Ullah, N., Khan, M.A., Khan, T., Ahmad, W., 2013. Cymbopogon citratus protects against the renal injury induced by toxic doses of aminoglycosides in rabbits. Indian J. Pharm. Sci. 75(2), 241-246.

Venditti, A., Bianco, A., 2018. Secondary metabolites of Hypericum richeri Vill. collected in Central Italy: chemotaxonomy and ethnomedicinal relevance. Trends Phytochem. Res. 2(3), 155-162.

Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2018. Bioactive essential oils from the Cameroonian rain forest: A review - Part I. Trends Phytochem. Res. 2(4), 187-234.

Wansi, J.D., Sewald, N., Nahar, L., Martin, C., Sarker, S.D., 2019. Bioactive essential oils from the Cameroonian rain forest: A review - Part II. Trends Phytochem. Res. 3(1), 3-52.

Xiao-Li, B., Hui-Hui, Y., Cheng-Zhong, W., Wei, F., Min-BO, L., 2015. C. citratus with anti-tumor and immunomodulatory activity. Pharm. Biol. 53(1), 117-124.

Yoon, W.J., Lee, N.H., Hyun, C.G., 2010. Limonese suppresses lipopolysaccharide-induced production nitric oxide, protasglandin E₂, and pro-inflammatory cytokines in RAW 264.7 macrophages. J. Oleo. Sci. 56, 415-421.