UPLC in phytochemical analysis
الموضوعات :
Lutfun Nahar
1
,
Satyajit D. Sarker
2
1 - Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom
2 - Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, John Moores University, James Parsons Building, Byrom Street, Liverpool L3 3AF, United Kingdom
الکلمات المفتاحية: liquid chromatography, Ultra Performance Liquid Chromatography (UPLC), analyte, column packed,
ملخص المقالة :
Ultra Performance Liquid Chromatography (UPLC), which has been around just over one and a half decade, is an advanced liquid chromatographic technique that offers a significantly short analysis time and small amount of solvent(s) as a mobile phase. It also provides much better separation efficiency and resolution of analyte mixtures.
Cotrut, R., Bădulescu, L., 2014. UPLC rapid quantification of ascorbic acid in several fruits and vegetables extracted using different solvents. Agric. Agric. Sci. Procedia 10, 160-166.
Dong, F., Hu, J., Shi, Y., Liu, M., Zhang, Q., Ruan, J., 2019. Effects of nitrogen supply on flavonols glycoside biosynthesis and accumulation in tea leaves (Camellia sinensis). Plant Physiol. Biochem. 138, 48-57.
Li, Y. F., Yang, P. Y., Luo, Y. H., Gao, B. Y., Sun, J. H., Lu, W. Y., Liu, J., Chen, P., Zhang, Y. Q., Yu, L. L. L., 2019a. Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties. Food Chem. 286, 8-16.
Li, T., Ye, W., Huang, B., Lu, X., Chen, X., Lin, Y., Wen, C., Wang, X., 2019. Determination and pharmacokinetic study of echinatin by UPLC-MS/MS in rat plasma. J. Pharm. Biomed. Anal. 168, 133-137.
Lu, M., Hu, Q. M., Zhang, Y. T., Zhai, Y. J., Zhou, Y. Q., Jiang, J. Q., 2019. Comparative chemical profiling of three TCM drugs in the Paeoniaceae family by UPLC-MS/MS combined with chemometric methods. Biochem. Syst. Ecol. 83, 121-129.
Nováková, L., Matysová, L., Solich, P., 2006. Advantages of application of UPLC in pharmaceutical analysis. Talanta 68, 908-918.
Sarker, S. D., Nahar, L., 2018. Computational Phytochemistry, Elsevier, U.S.A.
Zhou, P., Hu, O., Fu, H. Y., Ouyaang, L. Q., Gong, X. D., Meng, P., Wang, Z., Dai, M., Guo, X. M., Wang, Y., 2019. UPLC-Q-TOF/MS-based untargeted metabolomics coupled with chemometrics approach for Tieguanyin tea with seasonal and year variations. Food Chem. 283, 73-82.
