HPLC profile, antioxidant, antibacterial, and anti-proliferative activity of aerial parts of Ferula ovina (Boiss.) Boiss.
الموضوعات :Mohamad Hassan Mohamadi 1 , Behnam Mahdavi 2 , Esmaeil Rezaei-Seresht 3 , Sahar Riahi-Farsani 4 , Nazanin Entezari 5
1 - Department of Chemistry, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
2 - Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
3 - Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
4 - Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
5 - Department of Anesthesiology, Faculty of Paramedical, Sabzevar University of Medical Sciences, Sabzevar, Iran
الکلمات المفتاحية: Radical scavenging activity, Antibacterial activity, antioxidant activity, Ferrous ion chelating, Ferula ovina,
ملخص المقالة :
The aerial parts of Ferula ovina (Boiss.) Boiss. were extracted by methanol and then fractionated with solvents with different polarity. The evaluation of the bioactivity of the plant extracts including antioxidant, antimicrobial and antiproliferative activity was carried out using various assays. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. The leaves showed the highest total phenolic content and radical scavenging activity; the flowers had the maximum amount of total flavonoid content, and the stems exhibited the maximum ferrous ion chelating ability. Among ten microorganisms, the plant extract prevented the growth of three strains, namely Pseudomonas aeruginosa, Acinetobacter baumannii, and Streptococcus pneumonia. The flower of F. ovina (Boiss.) Boiss shows the best anti-human cervical carcinoma property against HeLa cell lines. Gallic acid was found as the major phenolic compound in the plant extract according to the HPLC analysis.
Abedi, D., Jalali, M., Sadeghi, N., 2009. Composition and antimicrobial activity of oleogumresin of Ferula gumosa Bioss. essential oil using Alamar Blue™. Res. Pharm. Sci. 3(1), 41-45.
Al-Khalil, S., Aqel, M., Afifi, F., Al-Eisawi, D., 1990. Effects of an aqueous extract of Ferula ovina on rabbit and guinea pig smooth muscle. J. Ethnopharmacol. 30(1), 35-42.
Arnoldi, L., Ballero, M., Fuzzati, N., Maxia, A., Mercalli, E., Pagni, L., 2004. HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots. Fitoterapia 75(3-4), 342-354.
Azarnivand, H., Alikhah-Asl, M., Jafari, M., Arzani, H., Amin, G., Mousavi, S.S., 2011. Comparison of essential oils from Ferula ovina (Boiss.) aerial parts in fresh and dry stages. J. Essent. Oil-Bear. Plants 14(2), 250-254.
Bagheri, S., Dashti-R, M., Morshedi, A., 2014. Antinociceptive effect of Ferula assa-foetida oleo-gum-resin in mice. Res. Pharm. Sci. 9(3), 207.
Bashir, S., Alam, M., Ahmad, B., Aman, A., 2014. Antibacterial, anti-fungal and phytotoxic activities of Ferula narthex Boiss. Pak. J. Pharm. Sci. 27(6), 1819-1825.
Dehpour, A.A., Ebrahimzadeh, M.A., Fazel, N.S., Mohammad, N.S., 2009. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas Aceites 60(4), 405-412.
Farhan, H., Rammal, H., Hijazi, A., Hamad, H., Daher, A., Reda, M., Badran, B., 2012. In vitro antioxidant activity of ethanolic and aqueous extracts from crude Malva parviflora L. grown in Lebanon. Asian J. Pharm. Clin. Res. 5(3), 234-238.
Ghannadi, A., Sajjadi, S.E., Beigihasan, A., 2002. Composition of the essential oil of Ferula ovina (Boiss.) Boiss. from Iran. DARU J. Pharm. Sci. 10(4), 165-167.
Hassan, S., Al Aqil, A., Attimarad, M., 2013. Determination of crude saponin and total flavonoids content in guar meal. Adv. Med. Plant. Res. 1(2), 24-28.
Hsu, J.-D., Kao, S.-H., Ou, T.-T., Chen, Y.-J., Li, Y.-J., Wang, C.-J., 2011. Gallic acid induces G2/M phase arrest of breast cancer cell MCF-7 through stabilization of p27Kip1 attributed to disruption of p27Kip1/Skp2 complex. J. Agric. Food Chem. 59(5), 1996-2003.
Iranshahi, M., Amanolahi, F., Schneider, B., 2012. New sesquiterpene coumarin from the roots of Ferula latisecta. Avicenna J. Phytomed. 2(3), 133.
Iranshahi, M., Basarloo, C., Piasenteh, S., 2010a. Purification and identification of compounds in the root Coma (Ferula ovina Boiss ). J. Med. Plants 36, 131-136.
Iranshahi, M., Ghiadi, M., Sahebkar, A., Rahimi, A., Bassarello, C., Piacente, S., Pizza, C., 2010b. Badrakemonin, a new eremophilane-type sesquiterpene from the roots of Ferula badrakema Kos.-Pol. Iran. J. Pharm. Res. (4), 275-279.
Iriti, M., Kubina, R., Cochis, A., Sorrentino, R., Varoni, E.M., Kabała‐Dzik, A., Azzimonti, B., Dziedzic, A., Rimondini, L., Wojtyczka, R.D., 2017. Rutin, a quercetin glycoside, restores chemosensitivity in human breast cancer cells. Phytother. Res. 31(10), 1529-1538.
Keshtkar, H., Azarnivand, H., Etemad, V., Moosavi, S., 2008. Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosa. Desert 13(1), 45-51.
Kose, E.O., Sarikuuml, C., 2010. Chemical composition, antimicrobial and antioxidant activity of essential oil of endemic Ferula lycia Boiss. J. Med. Plants Res. 4(17), 1698-1703.
Li, M., Behnam, M., Yaacob Wan, A., Din Laily, B., 2020. Chemical Composition, Antioxidant, Antimicrobial and Anti proliferative Activities of Essential Oil and Extract of the Fruits of Etlinger sayapensis. J. Essent. Oil-Bear. Plants 23(5), 931-943.
Mahdavi, B., Yaacob, W., Din, L.B., Jahangirian, H., 2013. Antioxidant activity of consecutive extracts of the base, stem and leaves of Etlingera brevilabrum. Asian J. Chem. 25(7), 3937-3941.
Mahdavi, B., Yaacob, W., Din, L.B., Nazlina, I., 2012. Antimicrobial activity of consecutive extracts of Etlingera brevilabrum. Sains Malays. 41, 1233-1237.
Nabavi, S.F., Ebrahimzadeh, M.A., Nabavi, S.M., Eslami, B., 2010. Antioxidant activity of flower, stem and leaf extracts of Ferula gummosa Boiss. Grasas Aceites 61(3), 244-250.
Prakash, A., Rigelhof, F., MIller, E., 2001. Antioxidant activity. Medallion laboratories analytical progress 19(2), 1-4.
Rocha, L.D., Monteiro, M.C., Teodoro, A.J., 2012. Anticancer properties of hydroxycinnamic acids-a review. J. Cancer Res. Clin. Oncol. 1(2), 109-121.
Sahebkar, A., Iranshahi, M., 2010. Biological activities of essential oils from the genus Ferula (Apiaceae). Asian Biomed. 4(6), 835-847.
Sahebkar, A., Iranshahi, M., 2011. Volatile constituents of the genus Ferula (Apiaceae): A review. J. Essent. Oil-Bear. Plants 14(5), 504-531.
Tirzitis, G., Bartosz, G., 2010. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochim. Pol. 57(2).
Wu, Q., Needs, P.W., Lu, Y., Kroon, P.A., Ren, D., Yang, X., 2018. Different antitumor effects of quercetin, quercetin-3′-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells. Food Funct. 9(3), 1736-1746.
Zhou, X., Huang, N., Chen, W., Xiaoling, T., Mahdavi, B., Raoofi, A., Mahdian, D., Atabati, H., 2020. HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways. AMB Express 10(1), 1-11.
