تاثیر تمرین ترکیبی و مکمل کافئین بر گیرندههای شبه گذرگاهی 2 و4 و دکتین-1 در مردان چاق
الموضوعات :شیرین قادری گودرزی 1 , آسیه عباسی دلویی 2 , احمد عبدی 3 , ایوب سعیدی 4
1 - دانشجوی دکتری گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 - دانشیار گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
3 - دانشیار گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
4 - استادیار گروه فیزیولوژی ورزشی واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
الکلمات المفتاحية: التهاب مزمن, تمرین ترکیبی, چاقی, کافئین,
ملخص المقالة :
مقدمه: اضافه‌وزن و چاقی با افزایش شرایط التهابی و اختلالات متابولیکی همراه است. عامل درمانی که بتواند اختلالات متابولیکی و ایمنی را سرکوب کند، ممکن است به طور موثری ناهنجاری های موازی در چاقی را بهبود بخشد. هدف از این تحقیق، بررسی تاثیر تمرین ترکیبی و مکمل کافئین بر گیرنده های شبه گذرگاهی 2 و4 و دکتین-1 در مردان چاق بود.مواد و روش ها: در یک کارآزمایی نیمه تجربی، 44 مرد چاق به صورت هدفمند و در دسترس انتخاب و به صورت تصادفی به چهار گروه 1) کنترل، 2) تمرین ترکیبی، 3) کافئین و 4) تمرین ترکیبی- کافئین تقسیم شدند. آزمودنی ها کافئین روزانه شش میلی گرم به ازای هر کیلوگرم وزن بدن به مدت 12 هفته دریافت کردند. برنامه تمرین ترکیبی شامل تمرین مقاومتی دایره ای (3 نوبت با 14 تکرار در50% درصد یک تکرار بیشینه و30 ثانیه استراحت بین هر ایستگاه) و تمرین هوازی (با شدت70% حداکثر ضربان قلب به مدت 30 دقیقه) 3 جلسه در هفته و به مدت 12 هفته اجرا شد. داده ها به روش آزمون تحلیل کوواریانس و آزمون تعقیبی توکی در سطح معنی داری P<0.05 تجزیه و تحلیل شد. یافته ها: 12هفته مداخله تمرین ترکیبی، کافئین و تمرین ترکیبی-کافئین موجب کاهش معنی دار سطوح TLR2،TLR4 و دکتین-1 سرم شد (001/0=P). کاهش سطوح TLR2،TLR4 و دکتین-1 سرم در گروه تمرین ترکیبی نسبت به کافئین معنی دار بود(001/0=P). همچنین میزان اثر تعاملی تمرین ترکیبی و کافئین بیشتر از کافئین و تمرین به تنهایی بود.نتیجه‌گیری: با توجه به یافته ها، پیشنهاد می شود از مداخله تمرین ترکیبی به همراه مصرف کافئین به منظور مزایای آن در کاهش عوارض التهابی مرتبط با چاقی استفاده شود.
Castoldi, A., Andrade-Oliveira, V., Aguiar, C. F., Amano, M. T., Lee, J., Miyagi, M. T., Latância, M. T., Braga, T. T., da Silva, M. B., Ignácio, A., Carola Correia Lima, J. D., Loures, F. V., Albuquerque, J., Macêdo, M. B., Almeida, R. R., Gaiarsa, J. W., Luévano-Martínez, L. A., Belchior, T., Hiyane, M. I., Brown, G. D. & Câmara, N. (2017). Dectin-1 Activation Exacerbates Obesity and Insulin Resistance in the Absence of MyD88. Cell Reports, 19(11), 2272–2288.
Cortez-Espinosa, N., García-Hernández, M. H., Reynaga-Hernández, E., Cortés-García, J. D., Corral-Fernández, N. E., Rodríguez-Rivera, J. G., Bravo-Ramírez, A., González-Amaro, R. & Portales-Pérez, D. P. (2012). Abnormal expression and function of Dectin-1 receptor in type 2 diabetes mellitus patients with poor glycemic control (HbA1c>8%). Metabolism: Clinical and Experimental, 61(11), 1538–1546.
Cristofaro, P. & Opal, S. M. (2006). Role of Toll-like receptors in infection and immunity: clinical implications. Drugs, 66(1), 15–29.
de Alcântara Almeida, I., Mancebo Dorvigny, B., Souza Tavares, L., Nunes Santana, L. & Vitor Lima-Filho, J. (2021). Anti-inflammatory activity of caffeine (1,3,7-trimethylxanthine) after experimental challenge with virulent Listeria monocytogenes in Swiss mice. International Immunopharmacology, 100, 108090.
del Fresno, C., Soulat, D., Roth, S., Blazek, K., Udalova, I., Sancho, D., Ruland, J. & Ardavín, C. (2013). Interferon-β production via Dectin-1-Syk-IRF5 signaling in dendritic cells is crucial for immunity to C. albicans. Immunity, 38(6), 1176–1186.
Ebrahim, K., bassami, M., kolahdozi, S. & Karimnia Saheb, V. (2012). The Effects of Circuit Resistance Exercise on Fat and Carbohydrate Metabolism During Endurance Exercise in Overweight Men. Iranian Journal of Endocrinology and Metabolism, 14(3), 257-66 [In Persian].
Favere, K., Bosman, M., Delputte, P. L., Favoreel, H. W., Van Craenenbroeck, E. M., De Sutter, J., Witvrouwen, I., De Meyer, G., Heidbuchel, H. & Guns, P. (2021). A systematic literature review on the effects of exercise on human Toll-like receptor expression. Exercise Immunology Review, 27, 84–124.
Feng, Y. & Chao, W. (2011). Toll-like receptors and myocardial inflammation. International Journal of Inflammation, 170352.
Fernandez-Gonzalo, R., De Paz, J. A., Rodriguez-Miguelez, P., Cuevas, M. J. & González-Gallego, J. (2014). TLR4-mediated blunting of inflammatory responses to eccentric exercise in young women. Mediators of Inflammation, 479395.
Fontana, L., Klein, S. & Holloszy, J. O. (2010). Effects of long-term calorie restriction and endurance exercise on glucose tolerance,
insulin action, and adipokine production. Age (Dordrecht, Netherlands), 32(1), 97–108.
Francaux, M. (2009). Toll-like receptor signalling induced by endurance exercise. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 34(3):454–458.
Gantner, B. N., Simmons, R. M., Canavera, S. J., Akira, S. & Underhill, D. M. (2003). Collaborative induction of inflammatory responses by dectin-1 and Toll-like receptor 2. The Journal of Experimental Medicine, 197(9), 1107–1117.
Jessen, A., Buemann, B., Toubro, S., Skovgaard, I. M. & Astrup, A. (2005). The appetite-suppressant effect of nicotine is enhanced by caffeine. Diabetes, Obesity & Metabolism, 7(4), 327–333.
Kalia, N., Singh, J. & Kaur, M. (2021). The role of dectin-1 in health and disease. Immunobiology, 226 (2), 152071.
Kawai, T. & Akira, S. (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11(5), 373–384.
Kobayashi-Hattori, K., Mogi, A., Matsumoto, Y. & Takita, T. (2005). Effect of caffeine on the body fat and lipid metabolism of rats fed on a high-fat diet. Bioscience, Biotechnology, and Biochemistry, 69(11), 2219–2223.
Kolb, H., Martin, S. & Kempf, K. (2021). Coffee and Lower Risk of Type 2 Diabetes: Arguments for a Causal Relationship. Nutrients, 13(4), 1144.
Krausgruber, T., Blazek, K., Smallie, T., Alzabin, S., Lockstone, H., Sahgal, N., Hussell, T., Feldmann, M. & Udalova, I. A. (2011). IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses. Nature Immunology, 12(3), 231–238.
Lee W. J. (2011). IGF-I Exerts an Anti-inflammatory Effect on Skeletal Muscle Cells through Down-regulation of TLR4 Signaling. Immune Network, 11(4), 223–226.
Liu, C. W., Tsai, H. C., Huang, C. C., Tsai, C. Y., Su, Y. B., Lin, M. W., Lee, K. C., Hsieh, Y. C., Li, T. H., Huang, S. F., Yang, Y. Y., Hou, M. C., Lin, H. C., Lee, F. Y. & Lee, S. D. (2018). Effects and mechanisms of caffeine to improve immunological and metabolic abnormalities in diet-induced obese rats. American journal of physiology. Endocrinology and Metabolism, 314(5), E433–E447.
McFarlin, B. K., Flynn, M. G., Campbell, W. W., Craig, B. A., Robinson, J. P., Stewart, L. K., Timmerman, K. L. & Coen, P. M. (2006). Physical activity status, but not age, influences inflammatory biomarkers and toll-like receptor 4. The journals of gerontology. Series A, Biological Sciences and Medical Sciences, 61(4), 388–393.
McFarlin, B. K., Flynn, M. G., Campbell, W. W., Stewart, L. K. & Timmerman, K. L. (2004). TLR4 is lower in resistance-trained older women and related to inflammatory cytokines. Medicine and Science in Sports and Exercise, 36(11), 1876–1883.
Medzhitov, R. (2001). Toll-like receptors and innate immunity. Nature Reviews Immunology, 1(2), 135–145.
Pahl, H. L. (1999). Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene, 18(49), 6853–6866.
Reid, D. M., Montoya, M., Taylor, P. R., Borrow, P., Gordon, S., Brown, G. D. & Wong, S. Y. (2004). Expression of the{beta}-glucan receptor, Dectin-1, on murine leukocytes insitu correlates with its function in pathogen recognition andreveals potential roles in leukocyte interactions. Journal of Leukocyte Biology, 76, 86–94.
Rodas, L., Riera-Sampol, A., Aguilo, A., Martínez, S. & Tauler, P. (2020). Effects of Habitual Caffeine Intake, Physical Activity Levels, and Sedentary Behavior on the Inflammatory Status in a Healthy Population. Nutrients, 12(8), 2325.
Rodriguez-Miguelez, P., Fernandez-Gonzalo, R., Collado, P. S., Almar, M., Martinez-Florez, S., de Paz, J. A., González-Gallego, J. & Cuevas, M. J. (2015). Whole-body vibration improves the anti-inflammatory status in elderly subjects through toll-like receptor 2 and 4 signaling pathways. Mechanisms of Ageing and Development, 150, 12–19.
Rosa, J. C., Lira, F. S., Eguchi, R., Pimentel, G. D., Venâncio, D. P., Cunha, C. A., Oyama, L. M., De Mello, M. T., Seelaender, M. & do Nascimento, C. M. (2011). Exhaustive exercise increases inflammatory response via Toll like receptor-4 and NF-κBp65 pathway in rat adipose tissue. Journal of Cellular Physiology, 226(6), 1604–1607.
Ruffino, J. S., Davies, N. A., Morris, K., Ludgate, M., Zhang, L., Webb, R. & Thomas, A. W. (2016). Moderate-intensity exercise alters markers of alternative activation in circulating monocytes in females: a putative role for PPARγ. European Journal of Applied Physiology, 116(9), 1671–1682.
Schubert, M. M., Hall, S., Leveritt, M., Grant, G., Sabapathy, S. & Desbrow, B. (2014). Caffeine consumption around an exercise bout: effects on energy expenditure, energy intake, and exercise enjoyment. Journal of applied physiology (Bethesda, Md. : 1985), 117(7), 745–754
Thiagarajan, P. S., Yakubenko, V. P., Elsori, D. H., Yadav, S. P., Willard, B., Tan, C. D., Rodriguez, E. R., Febbraio, M. & Cathcart, M. K. (2013). Vimentin is an endogenous ligand for the pattern recognition receptor Dectin-1. Cardiovascular Research, 99(3), 494–504.
Wang, Z., Ni, X., Zhang, L., Sun, L., Zhu, X., Zhou, Q., Yang, Z. & Yuan, H. (2020). Toll-Like Receptor 4 and Inflammatory Micro-Environment of Pancreatic Islets in Type-2 Diabetes Mellitus: A Therapeutic Perspective. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 4261–4272.
Willment, J. A., Marshall, A. S., Reid, D. M., Williams, D. L., Wong, S. Y., Gordon, S. & Brown, G. D. (2005). The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells. European Journal of Immunology, 35(5), 1539–1547.
Wu, H. & Ballantyne, C. M. (2020). Metabolic Inflammation and Insulin Resistance in Obesity. Circulation Research, 126(11), 1549–1564.
Yamauchi, R., Kobayashi, M., Matsuda, Y., Ojika, M., Shigeoka, S., Yamamoto, Y., Tou, Y., Inoue, T., Katagiri, T., Murai, A. & Horio, F. (2010). Coffee and caffeine ameliorate hyperglycemia, fatty liver, and inflammatory adipocytokine expression in spontaneously diabetic KK-Ay mice. Journal of Agricultural and Food Chemistry, 58(9), 5597–5603.
Zanchi, N. E., Lira, F. S., de Siqueira Filho, M. A., Rosa, J. C., de Oliveira Carvalho, C. R., Seelaender, M., Santos, R. V. & Lancha, A. H. (2010). Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle. European Journal of Applied Physiology, 109(6), 1095–1102.
Zhang, S. J., Li, Y. F., Wang, G. E., Tan, R. R., Tsoi, B., Mao, G. W., Zhai, Y. J., Cao, L. F., Chen, M., Kurihara, H., Wang, Q. & He, R. R. (2015). Caffeine ameliorates high energy diet-induced hepatic steatosis: sirtuin 3 acts as a bridge in the lipid metabolism pathway. Food & Function, 6, 2578–2587.
_||_Castoldi, A., Andrade-Oliveira, V., Aguiar, C. F., Amano, M. T., Lee, J., Miyagi, M. T., Latância, M. T., Braga, T. T., da Silva, M. B., Ignácio, A., Carola Correia Lima, J. D., Loures, F. V., Albuquerque, J., Macêdo, M. B., Almeida, R. R., Gaiarsa, J. W., Luévano-Martínez, L. A., Belchior, T., Hiyane, M. I., Brown, G. D. & Câmara, N. (2017). Dectin-1 Activation Exacerbates Obesity and Insulin Resistance in the Absence of MyD88. Cell Reports, 19(11), 2272–2288.
Cortez-Espinosa, N., García-Hernández, M. H., Reynaga-Hernández, E., Cortés-García, J. D., Corral-Fernández, N. E., Rodríguez-Rivera, J. G., Bravo-Ramírez, A., González-Amaro, R. & Portales-Pérez, D. P. (2012). Abnormal expression and function of Dectin-1 receptor in type 2 diabetes mellitus patients with poor glycemic control (HbA1c>8%). Metabolism: Clinical and Experimental, 61(11), 1538–1546.
Cristofaro, P. & Opal, S. M. (2006). Role of Toll-like receptors in infection and immunity: clinical implications. Drugs, 66(1), 15–29.
de Alcântara Almeida, I., Mancebo Dorvigny, B., Souza Tavares, L., Nunes Santana, L. & Vitor Lima-Filho, J. (2021). Anti-inflammatory activity of caffeine (1,3,7-trimethylxanthine) after experimental challenge with virulent Listeria monocytogenes in Swiss mice. International Immunopharmacology, 100, 108090.
del Fresno, C., Soulat, D., Roth, S., Blazek, K., Udalova, I., Sancho, D., Ruland, J. & Ardavín, C. (2013). Interferon-β production via Dectin-1-Syk-IRF5 signaling in dendritic cells is crucial for immunity to C. albicans. Immunity, 38(6), 1176–1186.
Ebrahim, K., bassami, M., kolahdozi, S. & Karimnia Saheb, V. (2012). The Effects of Circuit Resistance Exercise on Fat and Carbohydrate Metabolism During Endurance Exercise in Overweight Men. Iranian Journal of Endocrinology and Metabolism, 14(3), 257-66 [In Persian].
Favere, K., Bosman, M., Delputte, P. L., Favoreel, H. W., Van Craenenbroeck, E. M., De Sutter, J., Witvrouwen, I., De Meyer, G., Heidbuchel, H. & Guns, P. (2021). A systematic literature review on the effects of exercise on human Toll-like receptor expression. Exercise Immunology Review, 27, 84–124.
Feng, Y. & Chao, W. (2011). Toll-like receptors and myocardial inflammation. International Journal of Inflammation, 170352.
Fernandez-Gonzalo, R., De Paz, J. A., Rodriguez-Miguelez, P., Cuevas, M. J. & González-Gallego, J. (2014). TLR4-mediated blunting of inflammatory responses to eccentric exercise in young women. Mediators of Inflammation, 479395.
Fontana, L., Klein, S. & Holloszy, J. O. (2010). Effects of long-term calorie restriction and endurance exercise on glucose tolerance,
insulin action, and adipokine production. Age (Dordrecht, Netherlands), 32(1), 97–108.
Francaux, M. (2009). Toll-like receptor signalling induced by endurance exercise. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 34(3):454–458.
Gantner, B. N., Simmons, R. M., Canavera, S. J., Akira, S. & Underhill, D. M. (2003). Collaborative induction of inflammatory responses by dectin-1 and Toll-like receptor 2. The Journal of Experimental Medicine, 197(9), 1107–1117.
Jessen, A., Buemann, B., Toubro, S., Skovgaard, I. M. & Astrup, A. (2005). The appetite-suppressant effect of nicotine is enhanced by caffeine. Diabetes, Obesity & Metabolism, 7(4), 327–333.
Kalia, N., Singh, J. & Kaur, M. (2021). The role of dectin-1 in health and disease. Immunobiology, 226 (2), 152071.
Kawai, T. & Akira, S. (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11(5), 373–384.
Kobayashi-Hattori, K., Mogi, A., Matsumoto, Y. & Takita, T. (2005). Effect of caffeine on the body fat and lipid metabolism of rats fed on a high-fat diet. Bioscience, Biotechnology, and Biochemistry, 69(11), 2219–2223.
Kolb, H., Martin, S. & Kempf, K. (2021). Coffee and Lower Risk of Type 2 Diabetes: Arguments for a Causal Relationship. Nutrients, 13(4), 1144.
Krausgruber, T., Blazek, K., Smallie, T., Alzabin, S., Lockstone, H., Sahgal, N., Hussell, T., Feldmann, M. & Udalova, I. A. (2011). IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses. Nature Immunology, 12(3), 231–238.
Lee W. J. (2011). IGF-I Exerts an Anti-inflammatory Effect on Skeletal Muscle Cells through Down-regulation of TLR4 Signaling. Immune Network, 11(4), 223–226.
Liu, C. W., Tsai, H. C., Huang, C. C., Tsai, C. Y., Su, Y. B., Lin, M. W., Lee, K. C., Hsieh, Y. C., Li, T. H., Huang, S. F., Yang, Y. Y., Hou, M. C., Lin, H. C., Lee, F. Y. & Lee, S. D. (2018). Effects and mechanisms of caffeine to improve immunological and metabolic abnormalities in diet-induced obese rats. American journal of physiology. Endocrinology and Metabolism, 314(5), E433–E447.
McFarlin, B. K., Flynn, M. G., Campbell, W. W., Craig, B. A., Robinson, J. P., Stewart, L. K., Timmerman, K. L. & Coen, P. M. (2006). Physical activity status, but not age, influences inflammatory biomarkers and toll-like receptor 4. The journals of gerontology. Series A, Biological Sciences and Medical Sciences, 61(4), 388–393.
McFarlin, B. K., Flynn, M. G., Campbell, W. W., Stewart, L. K. & Timmerman, K. L. (2004). TLR4 is lower in resistance-trained older women and related to inflammatory cytokines. Medicine and Science in Sports and Exercise, 36(11), 1876–1883.
Medzhitov, R. (2001). Toll-like receptors and innate immunity. Nature Reviews Immunology, 1(2), 135–145.
Pahl, H. L. (1999). Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene, 18(49), 6853–6866.
Reid, D. M., Montoya, M., Taylor, P. R., Borrow, P., Gordon, S., Brown, G. D. & Wong, S. Y. (2004). Expression of the{beta}-glucan receptor, Dectin-1, on murine leukocytes insitu correlates with its function in pathogen recognition andreveals potential roles in leukocyte interactions. Journal of Leukocyte Biology, 76, 86–94.
Rodas, L., Riera-Sampol, A., Aguilo, A., Martínez, S. & Tauler, P. (2020). Effects of Habitual Caffeine Intake, Physical Activity Levels, and Sedentary Behavior on the Inflammatory Status in a Healthy Population. Nutrients, 12(8), 2325.
Rodriguez-Miguelez, P., Fernandez-Gonzalo, R., Collado, P. S., Almar, M., Martinez-Florez, S., de Paz, J. A., González-Gallego, J. & Cuevas, M. J. (2015). Whole-body vibration improves the anti-inflammatory status in elderly subjects through toll-like receptor 2 and 4 signaling pathways. Mechanisms of Ageing and Development, 150, 12–19.
Rosa, J. C., Lira, F. S., Eguchi, R., Pimentel, G. D., Venâncio, D. P., Cunha, C. A., Oyama, L. M., De Mello, M. T., Seelaender, M. & do Nascimento, C. M. (2011). Exhaustive exercise increases inflammatory response via Toll like receptor-4 and NF-κBp65 pathway in rat adipose tissue. Journal of Cellular Physiology, 226(6), 1604–1607.
Ruffino, J. S., Davies, N. A., Morris, K., Ludgate, M., Zhang, L., Webb, R. & Thomas, A. W. (2016). Moderate-intensity exercise alters markers of alternative activation in circulating monocytes in females: a putative role for PPARγ. European Journal of Applied Physiology, 116(9), 1671–1682.
Schubert, M. M., Hall, S., Leveritt, M., Grant, G., Sabapathy, S. & Desbrow, B. (2014). Caffeine consumption around an exercise bout: effects on energy expenditure, energy intake, and exercise enjoyment. Journal of applied physiology (Bethesda, Md. : 1985), 117(7), 745–754
Thiagarajan, P. S., Yakubenko, V. P., Elsori, D. H., Yadav, S. P., Willard, B., Tan, C. D., Rodriguez, E. R., Febbraio, M. & Cathcart, M. K. (2013). Vimentin is an endogenous ligand for the pattern recognition receptor Dectin-1. Cardiovascular Research, 99(3), 494–504.
Wang, Z., Ni, X., Zhang, L., Sun, L., Zhu, X., Zhou, Q., Yang, Z. & Yuan, H. (2020). Toll-Like Receptor 4 and Inflammatory Micro-Environment of Pancreatic Islets in Type-2 Diabetes Mellitus: A Therapeutic Perspective. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 4261–4272.
Willment, J. A., Marshall, A. S., Reid, D. M., Williams, D. L., Wong, S. Y., Gordon, S. & Brown, G. D. (2005). The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells. European Journal of Immunology, 35(5), 1539–1547.
Wu, H. & Ballantyne, C. M. (2020). Metabolic Inflammation and Insulin Resistance in Obesity. Circulation Research, 126(11), 1549–1564.
Yamauchi, R., Kobayashi, M., Matsuda, Y., Ojika, M., Shigeoka, S., Yamamoto, Y., Tou, Y., Inoue, T., Katagiri, T., Murai, A. & Horio, F. (2010). Coffee and caffeine ameliorate hyperglycemia, fatty liver, and inflammatory adipocytokine expression in spontaneously diabetic KK-Ay mice. Journal of Agricultural and Food Chemistry, 58(9), 5597–5603.
Zanchi, N. E., Lira, F. S., de Siqueira Filho, M. A., Rosa, J. C., de Oliveira Carvalho, C. R., Seelaender, M., Santos, R. V. & Lancha, A. H. (2010). Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle. European Journal of Applied Physiology, 109(6), 1095–1102.
Zhang, S. J., Li, Y. F., Wang, G. E., Tan, R. R., Tsoi, B., Mao, G. W., Zhai, Y. J., Cao, L. F., Chen, M., Kurihara, H., Wang, Q. & He, R. R. (2015). Caffeine ameliorates high energy diet-induced hepatic steatosis: sirtuin 3 acts as a bridge in the lipid metabolism pathway. Food & Function, 6, 2578–2587.
