روش هاي نوين ارزيابي و پایش مولكولي انتروویروس‌ها به عنوان شاخص ویروسی در محيط هاي آبي
الموضوعات : Virology
رضا صدیقی شیری
1
,
محمد کارگر
2
,
فرشید کفیل زاده
3
,
نیلوفر نیسی
4
1 - گروه میکروبیولوژی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران.
2 - گروه میکروبیولوژی، موسسه آموزش عالی زند، شیراز، ایران.
3 - دانشگاه آزاد اسلامی، واحد جهرم، گروه میکروبیولوژی
4 - مرکز تحقیقات بیماری‌های عفونی و گرمسیری، پژوهشکده سلامت، گروه ویروس‌شناسی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی جندی‌شاپور، اهواز، ایران.
الکلمات المفتاحية: ویروس‌های منتقل شونده آب, انتروویروس‌ها, شاخص هاي ویروسي, پایش زیستی, روش هاي تغليظ ,
ملخص المقالة :
پایش کیفیت آب محیطی یک مسئله جهانی است و تلاش‌های زیادی برای مدیریت صحیح آن انجام می‌شود. انتروویروس‌ها یکی از مهم‌ترین ویروس‌های منتقل شونده از راه آب هستند. اين ويروس ها از طریق تخلیه فاضلاب، عملیات کشاورزی و آب های جاری از محل‌های دفن زباله با نفوذ به آب‌های سطحی و زیرزمینی، خطرات قابل توجهی را برای سلامت عمومی انسان‌ها، حیوانات و گونه‌های آبزی ایجاد می‌کنند. شناسایی زودهنگام، سریع و مؤثر انتروویروس‌های قابل کشت در سیستم‌های آبی برای اطمینان از سطح بهداشتی آب و اجرای راهبرد‌های مناسب تصفيه آب و فاضلاب ضروري هستند. پایش زیستی محیط‌های آبی با وجود هزینه کمتر سنجش شاخص های باكتريايی مانند كلي فرم هاي مدفوعي ، انتروكوكوس ها و كلاستريديوم پرفرنجنس، نمي تواند تضمين كننده عدم وجود ويروس هاي روده اي باشد زیرا به كلرزني و تصفيه مرحله سوم مقاوم هستند. انتروویروس‌ها يكي از شاخص‌های ويروسی پایش کیفیت آب و تصفيه فاضلاب هستند، كه هنوز به صورت گسترده و متداول مورد استفاده قرار نگرفته اند. در اين مقاله، با ارزيابي پايگاه های معتبر علمی، كفايت كاربردی انواع روش های جديد تغليظ و شناسايي مولكولی انتروويروس ها در آب و فاضلاب مورد ارزيابی قرار گرفته است.
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