بررسی تاثیر نانو ذرات خرفهL. Portulaca oleracea و عصاره باکتری پروبیوتیکLactobacillus plantarum بر روی رده سلولی CacoII بر روی بیان ژن های JAKI و JAKII
الموضوعات :
1 - گروه زیست‌شناسی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
الکلمات المفتاحية: پروبيوتيک, Lactobacillus plantarum, سرطان كولون, Portulaca oleracea, CacoII , JAKI, JAKII ,
ملخص المقالة :
چکیده: نانو ذرات خرفه Portulaca oleracea و باکتریهای پروبيوتيك که ميكروارگانيسمهای زنده و مفيد هستند با تأثير بر آنزيمهای گوارشی حيوانات و انسانها، مهار عوامل سرطانزا را در داخل بدن و شرايط آزمايشگاهی و تركيبات القا كننده سرطان و تومورها در حيوانات آزمايشگاهی نقش مؤثری در جهت مقابله با سرطان ايفا میكنند. هدف مطالعه حاضر بررسی تاثیر نانو ذرات خرفه Portulaca oleracea و عصاره باکتری پروبیوتیکLactobacillus plantarum روی رده سلولی CacoII بر روی بیان ژن های JAKI و JAKII میباشد.
مواد و روشها : در این مطالعه که به صورت تجربی انجام شده است از كشت باكتریLactobacillus plantarum، مایع رویی جدا شد.سپس اثرات سمیت سلولی مایع رویی جدا شده از باکتری و نانوذرات خرفه Portulaca oleracea همچنین باکتریها بر روی رده سلولی سرطان كولون CacoII در مدت زمان 24،48و72ساعت با استفاده از روشMTT بررسی شد.همچنین بیان ژنهایJAKII و JAKII با استفاده از روش Real Time PCR مورد بررسی قرار گرفت.
نتایج:. مایع رویی که حاوی باکتری Lactobacillus plantarum باعث کاهش تکثیر سلولی میشوند. نتايج به دست آمده نشان داد كه درصد مهاري محلول رویی (سوپرناتانت) Lactobacillus plantarum روي سلولهاي سرطانی CacoII ، ژنهای JAKI در طول 48 و ۷۲ ساعت افزایش بیان معناداری داشته است. ژنهای JAKII در طول 48 و ۷۲ ساعت کاهش بیان معناداری داشته است.
نتیجهگیری: ميتوان از باكتري Lactobacillus plantarum و نانوذرات خرفه Portulaca oleracea ، جهت ايجاد يك راهكار نوين درماني با تأثير بالا، عوارض جانبي پايين، بيخطر از نظر بيولوژيكي و هزينه كمتر براي درمان و همچنين پيشگيري از سرطان كولون بهره برد.
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