Comparative study of phytochemical composition, oral toxicity, antioxidant, and anticancer activities of both Aloe vera and Aloe vacillans (Asphodelaceae family) flowers extract: In vitro, in vivo, and in silico studies

الموضوعات :

Asma Mohsan Mohammed Al-Wajih ¹ , Amina El-Shaibany ² , Mahmoud Mahyoob Alburyhi ³ , Ahmed S. Abdelkhalek ⁴ , Mahmoud M. Elaasser ⁵ , Ali E. Raslan ^{6 *}

1 - Pharmacognosy Department, University of Sana’a, Pharmacy College, Sana’a, Yemen
2 - Pharmacognosy Department, University of Sana’a, Pharmacy College, Sana’a, Yemen
3 - Department of Pharmaceutics and Industrial Pharmacy, Pharmacy College, University of Sana’a, Sana’a, Yemen
4 - Medicinal Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
5 - The Regional Center for Mycology and Biotechnology, Al-Azhar University, 11787 Nasr City, Cairo, Egypt
6 - Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt

تاريخ الإرسال : 07 الثلاثاء , رجب, 1446 تاريخ التأكيد : 26 الأربعاء , رمضان, 1446 تاريخ الإصدار : 02 الأربعاء , ذو القعدة, 1446

الکلمات المفتاحية: Aloe vacillans, Aloe vera, Asphodelaceae family, Anticancer, Antioxidant, Oral toxicity, Phytochemical composition, In vitro, In vivo, In silico,

ملخص المقالة :

The research study aimed to compare the chemical composition, acute toxicity, antioxidant, and antiproliferative activities of extracts from Aloe vera and Aloe vacillans flowers. Gas chromatography-mass spectrometry (GC-MS) analysis identified 79 components in the n-hexane fraction of A. vera flowers (AVeFHF). The toxicity study indicated no mortality or morbidity at the tested concentrations for any of the mice treated with the investigated extracts. A. vera flowers methanolic extract (AVeFME) exhibited dose-dependent antioxidant activity. AVeFME demonstrated significant anticancer efficacy against HCT-116, Caco-2, A-549, and RD cell lines, with IC50 values of 14.0, 14.6, 12.0, and 14.7 µg/mL, respectively. AVeFHF exhibited moderate anticancer activity against HCT-116, A-549, and RD cell lines. Histological examination revealed substantial morphological changes consistent with the apoptotic mechanism of action. The molecular docking study provided insights into the binding modes of the identified compounds with EGFR and PARP-1 enzymes.

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