Antimicrobial effect of ethanolic extract of orange peel against bacteria isolated from burn infection

الموضوعات : Journal of Plant ecophysiology

Zeinab Fotoohiyan ¹ , Hadis Shahrokhi sardo ²

1 - Department of Plant pathology, Jiroft Branch, Islamic Azad University, Kerman, Iran
2 - Department of Microbiology, Jiroft Branch, Islamic Azad University, Kerman, Iran

تاريخ الإرسال : 22 الأحد , جمادى الأولى, 1446 تاريخ التأكيد : 26 الخميس , جمادى الأولى, 1446 تاريخ الإصدار : 25 السبت , شوال, 1445

الکلمات المفتاحية: Antibiotics, Plant extract, Orange peel, Antibacterial, Jiroft,

ملخص المقالة :

Objective: A burn is a type of damage to the skin. In this skin damage, the first defense barrier of the body is destroyed, and the patient's wound becomes the best environment for the growth of bacteria. Nowadays, medicinal plants are used to deal with bacteria. In this research, in order to investigate the effect of ethanolic extract of orange peel on bacteria isolated from the wounds of burn patients in Jiroft hospitals and compare them with selected antibiotics, in 2022. For this purpose, after collecting the orange peel of Thomson variety, its extract was performed by percolation method and after isolation, the bacteria causing the infection were identified using morphological and biochemical tests. Then, the effect of ethanolic extract of orange peel and common antibiotics against bacteria was investigated. The results showed that Pseudomonas aeruginosa (52%), Staphylococcus aureus (37%) and Escherichia coli (11%) frequency had the most role in burn wound infections. And the orange peel extract had a better antibacterial effect against bacteria compared to cefixime and azithromycin antibiotics, so that the diameter of the inhibition zone of orange peel extract in S. aureus at 300.12 mg/ml was 31.22 mm and the antibiotic cefixime and azithromycin were 17.23 and 27.52 mm, respectively. Therefore we can use orange peel extract instead of antibiotics and chemical drugs to fight the burn infection bacteria.

 

المصادر:

Abbasi, N. Mahdavi, S., 2016. Antioxidant and antimicrobial properties of essential oil and extract of Echinophora sibthorpiana on Escherichia coli and Staphylococcus aureus, Journal of Innovation in Food Science and Technology. 8: 3 (Persian).
Aljeboury Gh., 2013. In vitro antibacterial activity of Eucalyptus microtheca and Cinnamomum zeylanicum crude extttracts against Pseudomonas aeruginosa isolates. Medical Journal Of Babylon; 10: 784-93.
Ashook kumar K., Narayani M., Subanthini A. and jayakumar M. 2011. Antimicrobial Activity and phytochemical Analysis of citrus Fruit peels—Utilization Fruit Waste.IJEST. 3:5414-21.
Askarian, M., Hosseini, R.S., Kheirandish, P. and Assadian, O., 2004. Incidence and outcome of nosocomial infections in female burn patients in Shiraz, Iran. Am J Infect Control. 32(1): 23-6.
Avni T, Levcovich A, Ad-El DD, Leibovici L., and Paul M., 2010. Prophylactic antibiotics for burns patients: systematic review and meta-analysis". BMJ. 340:
Bagamboula C, Uyttendaele M, Debevere J. 2004. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol;21(1):32-42.
Behzadnia S, Davoudi A, Rezaee MS, Ahangarkani F. 2014., Nosocomial infection in pediatric population and antibiotic resistance of Iran. Iran Red Crescent Med J.16: e14562.
Brooks, Geo F; Butel, Janet S; Morse, Stephen A. 2002. Jawetz, Melnick, &Adelberg's Medical Microbiology. Salehi, S; Ebrahimi, V; Ebrahimi Vrkyany, M; Zare Mirzai, SA; And others. Tehran. Cultural Institute Publishing Timorzadeh–Publication Tabib. (Persian).
Cakir B , Yegen BC. 2004. Systemic responses to burn injury.Turkey J Med Sci. 34: 215-26.
Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V. 2000. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature 407 (6802): 340–8. doi:10.1038/35030019.
Chambers HF, 2001. The changing epidemiology of Staphylococcus aureus?. Emerg Infect Dis 7 (2): 178–82. doi:10.3201/eid0702.010204. PMC 2631711.
Clauditz A, Resch A, Wieland KP, Peschel A, Götz F 2006. Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. Infection and immunity 74 (8): 4950–3.
Darnton NC, Turner L, Rojevsky S, Berg HC (2007). "On torque and tumbling in swimming Escherichia coli". J. Bacteriol. 189 (5): 1756–64.
Faqri, J., 2005. Scientific Journal of Hamadan University of Medical Sciences.14: 3(Persian).
Fotadar U, Zaveloff P, Terracio L., 2005. Growth of Escherichia coli at elevated temperatures. J. Basic Microbiol. 45 (5): 403–4.
George D, and Bhat ss,, 2009. Antony B. comparative evaluation of the antimicrobial efficacy of Aloe vera tooth gel and two popular commercial toothpastes:An in vitro study.Gen Dent. 57(3):238-41.
Holems JH, Heimbach DM. B URNS. In: Brunicardi FC, Andersen DK, Billiar TR, Dunn DL, Hunter JG, Pollock RE(editors). 2005. Schwartzs principles of sutgery. 8th ed. New York: McGraw- Hill. 204-28.
Kummar B, Vijayakumar M, Govindarajan R, Pushpangadan P. 2007. Ethnopharmacological approaches to wound healing – exploring medicinal plants of India. J Ethnopharmacol; 114: 103– 30.
Lukjancenko O, Wassenaar TM, Ussery DW 2010. Comparison of 61 sequenced Escherichia coli genomes. Microb. Ecol. 60 (4): 708–20. doi:10.1007/s00248-010-9717-3. PMC 2974192.
Madigan MT, Martinko JM 2006. Brock Biology of microorganisms (11th ed.). (Pearson).
Mohamed H. 2016. One year prevalence of critically ill burn wound bacterial infections in surgical ICU in Egypt: Retrospective study. Egypt J Anaesth; 32:431-434
Nakhaei Moghaddam, M., 2009. Journal of Microbial Biotechnology, Islamic Azad University. 1(2) (Persian).
Narimani R, Moghaddam M. 2016. Investigation of antibacterial properties of ethanolic extracts of four native medicinal plants of Ardebil province by two methods of disk and well diffusion. Research in Medicine; 40 (3) :149-154.
Ogston, A. 1984. On Abscesses. Classics in Infectious Diseases. Rev Infect Dis, 6 (1): 122–28. PMID 6369479.
Osman AG, Koutb M, Sayed Ael-D. 2010. Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet-A radiation on African catfish Clarias gariepinus. Journal of photochemistry and Photobiology. 99:1-8.
Park JH, Heggie KM, Edgar DW, Bulsara MK, Wood FM. 2013. Does the type of skin replacement surgery influence the rate of infection in acute burn injured patients? Burns. 39:1386-1390.
Paton JC, Paton AW 1998. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections". Clin. Microbiol. Rev. 11 (3): 450–79.
Paul A, Cox PA. 1995. An ethnobotanical survey of the uses for Citrus aurantium. Economic Botany. 49 (3): 249-256.
Ranade AN, Wankhede SS, Ranpise NS, Mundada MS. 2012 Development of bilayer floating tablet of amoxicillin and Aloe vera gel powder for treatment of gastric ulcers. AAPS PharmSciTech. 13(4):1518-23.
Razmjoo, M., 2016. Iranian Journal of Nutrition Sciences & Food Technology. 11(1)(Persian).
Rezaeizadeh H, Alizadeh M, Naseri M, Ardakani MR. 2009. The Traditional Iranian Medicine Point of View on Health and Disease. Iran J Public Health. 38(1): 169-72.
Salavatifar, Maryam; Ahmadi, Seyedeh Meysameh; Todorov, Svetoslav Dimitrov; Khosravi-Darani, Kianoush; Tripathy, Abhishek 2023. Impact of Microgravity on Virulence, Antibiotic Resistance and Gene Expression in Beneficial and Pathogenic Microorganisms. Mini-Reviews in Medicinal Chemistry. 23 (16): 1608–1622.
Samsam Shariat, Hadi 1992. extracting and extracting effective substances of medicinal plants and their identification and evaluation methods 293 p.
Schultz L, Walker SA, Elligsen M, Walker SE, Simor A, Mubareka S, et al., 2013. Identification of predictors of early infection in acute burn patients. Burns. 39:1355-1366.
Shamloo, M., 2016. Evaluation of Antimicrobial Activity of Aqueous and Ethanolic Extracts of Aloe Vera on Pathogenic Bacteria (Staphylococcus aureus, Escherichia coli, Listeria monocytogenes) (Persian).
Taghi Akhi, M., 2009. Bacteria Involved in Burn Infections and Their Susceptibility Pattern
Vindenes,H. and Bjerknes, R.,1995. Microbial colonization of large wounds.Burns.21,pp.575-9
Wasiak, Jason; Cleland, Heather; Campbell, Fiona; Spinks, Anneliese 2013. Dressings for superficial and partial thickness burns. The Cochrane Database of Systematic Reviews.
Zareian P, Zahiri SH, Ketabchi F, Ruzmeh SH. 2007. Effect of local Tamarix monnifera on skin wound healing process in rabbit. J Mazand Univ Med Sci; 17: 48-57.
Zargari, A., 1997. Medicinal Plants, 4th volume, 6th edition, Tehran University Press. (Persian).
Mettam, G. R., & Adams, L. B. 1994. How to prepare an electronic version of your article. In B. S. Jones, & R. Z. Smith (Eds.), Introduction to the electronic age (pp. 281-304).New York: E-Publishing Inc.
Van der Geer, J., Hanraads, J. A. J., & Lupton R. A. 2000. The art of writing a scientific article. Journal of Scientific Communications, 163, 51-59.
Strunk, W., Jr., & White, E. B. 1979. The elements of style. (3rd ed.).New York: Macmillan, (Chapter 4).