Frequency of Class I, II Integrons, and Aminoglycoside- Resistance Genes in Clinical Isolates of Staphylococcus aureus in northern Iran
الموضوعات : Biotechnological Journal of Environmental Microbiologyمهسا آقایی 1 , لیلا اسدپور 2 , امیر آراسته 3
1 - گروه زیست شناسی، واحد رشت،دانشگاه آزاد اسلامی، رشت، ایران
2 - گروه زیست شناسی ، واحد رشت، دانشگاه آزاد اسلامی ، رشت ، ایران
3 - گروه زیست شناسی، واحد رشت ، دانشگاه آزاد اسلامی ، رشت ، ایران
الکلمات المفتاحية: S. aureus, aminoglycosides, integron, aac(6’)-Ie-aph(2”) , aph(3’)-IIIa , ant(4’)-Ia,
ملخص المقالة :
Staphylococcus aureus is known as a hospital pathogenic bacterium that can cause a wide range of infections.
Aminoglycosides are one of the drugs of choice in the treatment of septicemia caused by this bacterium.
The aim of this study is to investigate the level of resistance to aminoglycosides, determine the frequency of
aminoglycosides modifying enzyme genes and the frequency of class I and II integrons among clinical isolates
of aminoglycosides resistant S. aureus. In this study, the resistance of 200 isolates of S. aureus to aminoglycosides
including gentamycin, kanamycin, amikacin and streptomycin were investigated by Kirby-Boyer disc
diffusion method. The frequency of aac(6’)-Ie-aph(2”), aph(3’)-IIIa and ant(4’)-Ia genes and and class 1 and
2 integrons in test isolates were determined by PCR. Out of 200 isolates, 134 isolates (67%) were resistant to
at least one aminoglycoside. Of this number, the frequency of aac(6’)-Ie-aph(2”), aph(3’)-IIIa and ant(4’)-Ia
genes were 35.07%, 29.1% and 20%, respectively. Class I and II Integrons were detected in respectively 66%
and 19% of isolates. All of isolates carrying class I integron were aminoglycoside resistant and positive for
aminoglycoside modifying genes. The results showed high resistance to aminoglycosides and high frequency
of aminoglycoside modifying genes in clinical S. aureus isolates carrying calss I and II integrons.
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