بررسی توانایی آنتاگونیستی قارچ های اندوفیت ریشه و گونه های تریکودرما برروی قارچ Macrophomina phaseolinaدر شرایط آزمایشگاهی

الموضوعات : دو فصلنامه تحقیقات بیماریهای گیاهی

فریبا عباس زاده ¹ , ابراهیم محمدی گل تپه ² , ابراهیم پورجم ³ , امیر خراسانی ⁴ , یونس رضایی دانش ⁵ , آجت ورما ⁶

1 - گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.
2 - گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.
3 - گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.
4 - گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.
5 - گروه گیاهپزشکی ، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه ایران.
6 - گروه مطالعاتی گیاهان داروئی و میکروبی، دانشگاه آمیتی نویدا ایالت اترپرادش، هندوستان.

تاريخ الإرسال : 28 السبت , ذو الحجة, 1434 تاريخ التأكيد : 28 السبت , ذو الحجة, 1434 تاريخ الإصدار : 17 الإثنين , ذو القعدة, 1434

الکلمات المفتاحية: کنترل بیولوژیکی, Trichoderma spp, Sebacina vermifera, Piriformospora indica .Macrophomina phaseolina,

ملخص المقالة :

در این تحقیق، از دو قارچ اندوفیت ریشه (Sebacina vermifera و Piriformospora indica) و دو گونه تریکودرما
(T. viride )و Trichoderma harzianum (T-100) علیه قارچ Macrophomina phaseolina استفاده گردید . توانایی
آنتاگونیستی قارچ های اندوفیت ریشه و گونه های تریکودرما از طریق آزمایش های درون شیشه ای و به کمک روش کشت متقابل، کلنیزاسیون و تولید متابولیت های فرار مورد ارزیابی قرار گرفت مساحت پرگنه M. phaseolina. هر روز اندازه گیری و درصد کاهش رشد هر روز در مقایسه با تیمار شاهد محاسبه شد. در کشت متقابل، تیماری که شامل هر دو گروه قار چ های اندوفیت و گونه های تریکودرما بود، بیشترین اثر بازدارندگی را بر روی رشد میسلیومی M. phaseolina داشت. بررسی قدرت کلنیزاسیون نشان داد که قارچ های آنتاگونیست قادرند پرگنه M. phaseolinaرا در برگرفته و آن را تجز یه نمایند . نتایجآزمون متابولیت های فرار نیز مشخص نمود که قارچ های اندوفیت بر خلاف گونه های تریکودرما قادر به تولید متابولیتهای فرارنمی باشند.

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