Synthesis of ZnO: Ag nanoparticle and Evaluation its Antimicrobial Activity against Common Isolated Bacterial Pathogens from Dairy Products
الموضوعات : Biotechnological Journal of Environmental Microbiology
1 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان ، ایران
الکلمات المفتاحية: Nanoparticle, Zinc oxide, Staphylococcus, Escherichia coli, Antibiogram,
ملخص المقالة :
The most important feature of nanoparticles is to have a higher specific surface area ratio than their counterpart
with a larger size. The aim of this study was the synthesis of zinc oxide nanoparticles doped with silver and
its antimicrobial effects on common bacterial pathogens isolated from raw dairy products. In this research 45
samples of dairy raw products after dilution of samples, to isolate Staphylococcus aureus and Escherichia
coli was transferred to the Baird Parker Agar and Sorbitol Mac Conkey Agar media respectively and were
identified using a series of specific tests. Zinc oxide nano powder doped with silver was synthesized by sol-gel
method. Antimicrobial effects of nanoparticles were investigated by well diffusion method. Minimum inhibitory
concentration (MIC) of Zinc oxide-doped nano powder and minimum bactericidal concentration (MBC)
were determined. The mean diameter zone of the inhibitory growth of strains of E. coli PTCC 1399 , E. coli (1)
and E. coli (2) were 22.5, 18.5 and 15.4 mm respectively at a concentration of 50 mg / ml and mean diameter
zone of the inhibitory of S. aureus PTCC 1189, S. aureus (1) and S. aureus (2) standard strains were 24.5, 20.4
and 19.5 mm.In this concentration, MIC for E. coli PTCC 1399 was 1.75; E. coli (isolate 1) and E. coli (isolate
2) were 1.55 and 3.13 mg / ml, respectively. According to the present research, it can be concluded that the
zinc oxide nanoparticle itself has a good inhibitory effect on two strains of E. coli and Staphylococcus aureus.
One of the ways to improve or change the properties of nanostructures such as zinc oxide is to introduce impurities
into its structure. In case of further experiments, this nano particle can be used as preservative.
سالیانی، م . جلال، ر . گوهرشادی، ا.تاثیرات pH و دما بر فعالیت ضد باکتریایی نانو ذرات اکسیبد روی. پایان نامه کارشناسی ارشد. دانشگاه فردوسی مشهد. 1390. Azam, . A Ahmed, . A.S. Oves, M. Khan, M.S.. Habib, S.S Memic, A. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a Comparative study, Int. J. Nanomedicine 7 (2011) 6003–6009. Barzegari Firouzabadi F., Marzban Z., Khaleghizadeh S., Daneshmand F., Mirhosseini M. Combined effects of zinc oxide nanoparticle and malic acid to inhibit Escherichia coli and Staphylococcus aureus. Iran J Med Microbiol. 2016; 10 (5): 52-59. Emami-Karvani, Z. Chehrazi, P. Antibacterial activity of ZnO nanoparticle on Gram positive and gram-negative bacteria, Afr. J. Microbiol. Res. 5 (2011) 1368–1373. Espitia, Paula Judith Perez, et al. "Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications." Food and Bioprocess Technology 5.5 (2012): 1447-1464. Faramarzi T, Jonidi jafari A, Dehghani S, Mirzabeygi M, Naseh M, Rahbar Arasteh H. A Survey of Bacterial Contamination of Food Supply in the West of Tehran. Journal of Fasa University of Medical Sciences/ May 2012/ Vol.2/ No.1/ P 11-18. Ghosh, Tanushree, Anath Bandhu Das, Bijaylaxmi Jena, and Chinmay Pradhan. "Antimicrobial effect of silver zinc oxide (Ag-ZnO) nanocomposite particles." Frontiers in Life Science 8, no. 1 (2015): 47-54. Gündoğan N, Citak S, Turan E. Slime production, DNase activity and antibiotic resistance of Staphylococcus aureus isolated from raw milk, pasteurised milk and ice cream samples. Food Control. 2006;17(5):389-92. Hu, Yawei, Huirong He, Xia Kong, and Yangmin Ma. "Synthesis and Antibacterial Activities of Ag/ZnO Nanoparticles." In Key Engineering Materials, vol. 697, pp. 714-717. Trans Tech Publications, 2016. Matai, I., Sachdev, A., Dubey, P., Kumar, S. U., Bhushan, B., & Gopinath, P. (2014). Antibacterial activity and mechanism of Ag–ZnO nanocomposite on S. aureus and GFP-expressing antibiotic resistant E. coli. Colloids and Surfaces B: Biointerfaces, 115, 359-367.
Venkatasubramanian, K., & Sundaraj, S. (2014). Antibacterial activity of Zinc Oxide and Ag doped Zinc Oxide Nanoparticles against E. coli. Chem Sci Rev Lett, 3, 40-44
. Wang, Chao, Lian-Long Liu, Ai-Ting Zhang, Peng Xie, Jian-Jun Lu, and Xiao-Ting Zou. "Antibacterial effects of zinc oxide nanoparticles on Escherichia coli K88." African Journal of Biotechnology 11, no. 44 (2012): 10248-10254. Wang, Shilei, Jie Wu, Hao Yang, Xiangyu Liu, Qiaomu Huang, and Zhong Lu. "Antibacterial activity and mechanism of Ag/ZnO nanocomposite against anaerobic oral pathogen Streptococcus mutans." Journal of Materials Science: Materials in Medicine 28, no. 1 (2017): 23
.
