Effect of Lighting Schedule during Incubation on Embryonic Development and Bone Gene (Col10a1, Spp1, Bglap, and Alp) Expression in Broiler Hatchlings
الموضوعات :S.M. Hashemian 1 , M.J. Zamiri 2 , Z. Khaksar 3 , Z. Ansari Pirsaraei 4 , H.R. Moradi 5
1 - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
2 - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
3 - Department of Basic Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
4 - Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran
5 - Department of Basic Science, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
الکلمات المفتاحية: chondrocyte, collagen, crystallization, endocrine, light color, osteocalcin,
ملخص المقالة :
We studied the effect of lighting during 19 days of incubation on embryonic bone characteristics, gene ex-pression, thyroid hormones, and glucose in Cobb broiler hatchlings. Eggs (2160) were incubated under darkness (control) and green and white lighting (16D:8L and 12D:12L). On 19th day, tibiotarsal length in-creased under eight hours of white light, and femoral ossified length was longer under eight hours of white and green light. In femur, expression of Alp, Col10a1, and Spp1 increased by 12 h white and 8 h green lighting, and Bglap expression decreased under white. In tibiotarsus, expression of Alp decreased by 8 h lighting (green or white) while Bglap increased by 12 h lighting (green or white). Tibiotarsal expression of Col10a1 increased by 12 h lighting (white and green), and in 8 h green lighting, and Spp1 expression was higher under light illumination. The hatchling blood triiodothyronine concentration in 8 h green lighting was higher than that in control, whereas, thyroxine concentration was lower under 12 h white lighting com-pared with other treatments. Femoral organic matter to mineral (OM:M) ratio and calcium concentration were higher in the eight-hour white light group than in the control, but tibiotarsal OM:M ratio and calcium and phosphorus concentrations were higher under 12 h white light. The findings indicated that lighting dur-ing incubation may impact, both positively and negatively, bone morphology and gene expression. More studies are needed to find the most beneficial lighting system during incubation in chickens.
Anderson H.C., Sipe J.B., Hessle L., Dhamyamraju R., Elisa A., Camacho N.P. and Millán J.L. (2004). Impaired calcification around matrix vesicles of growth plate and bone in alkaline phosphatase-deficient mice. Am. J. Pathol. 164, 841-847.
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Archer G.S. (2017). Exposing broiler eggs to green, red and white light during incubation. Animal. 11, 1203-1209.
Barnard J.C., Williams A.J., Rabier B., Chassande O., Samarut J., Cheng S.Y., Bassett J.H. and Williams G.R. (2005). Thyroid hormones regulate fibroblast growth factor receptor signaling during chondrogenesis. Endocrinology. 146, 5568-5580.
Choruta A. (2013). Evaluation of densitometric and geometric parameters of the femur in 14-month-old ostriches depending on sex with the use of computed tomography. J. Vet. Res. 57, 287-291.
Cooper C.B., Voss M.A., Ardia D.P., Austin S.H. and Robinson W.D. (2011). Light increases the rate of embryonic development: implications for latitudinal trends in incubation period. Funct. Ecol. 25, 769-776.
Cui Y.M., Wanng J., Zhang H.J., Feng J., Wu S.G. and Qi G.H. (2019). Effect of photoperiod on growth performance and quality characteristics of tibia and femur in layer ducks during the pullet phase. Poult. Sci. 98, 1190-1201.
Dishon L., Avital-Cohen N., Malamud D., Heiblum R., Druyan S., Porter T.E., Gumułka M. and Rozenboim I. (2017). In ovo monochromatic green light photostimulation enhances embryonic somatotropic axis activity. Poult. Sci. 96, 1884-1890.
Duncan B.J.H. and Williams G.R. (2003). The molecular actions of thyroid hormone in bone. Trends Endocrinol. Metab. 14, 356-364.
Farquharson C. and Jefferies D. (2000). Chondrocytes and longitudinal bone growth: The development of tibial dyschondroplasia. Po
ult. Sci. 79, 994-1004.
Fornari M.B., Zanella R., Ibelli A.M.G., Fernandes L.T., Cantão M.E., Thomaz-Soccol V., Ledur M.C. and Peixoto J.O. (2014). Unraveling the associations of osteoprotegerin gene with production traits in a paternal broiler line. Springerplus. 3, 682-692.
Geng A.L., Zhang Y., Zhang J., Zeng L.C., Chang C., Wang H.H., Yan Z.X., Chu Q. and Liu H.G. (2021). Effects of light regime on the hatching performance, body development and serum biochemical indexes in Beijing You Chicken. Poult. Sci. 100, 101270-101281.
Gogakos A.I., Duncan B.J.H. and Williams G.R. (2010). Thyroid and bone. Arch. Biochem. Biophys. 503, 129-136.
Groves P.J. and Muir W.I. (2017). Earlier hatching time predisposes Cobb broiler chickens to tibial dyschondroplasia. Animal. 11, 112-120.
Halevy O., Piestun Y., Rozenboim I. and Yablonka-Reuveni Z. (2006). In ovo exposure to monochromatic green light promotes skeletal muscle cell proliferation and affects myofiber growth in posthatch chicks. Am. J. Physiol. Regul. 290, 1062-1070.
Khalil H.A. (2009). Productive and physiological responses of Japanese quail embryos to light regime during incubation period. Slovak J. Anim. Sci. 42, 79-86.
Kolakshyapati M., Flavel R.J., Sibanda T.Z., Schneider D., Welch M.C. and Ruhnke I. (2019). Various bone parameters are positively correlated with hen body weight while range access has no beneficial effect on tibia health of free-range layers. Poult. Sci. 98, 6241-6250.
Lu J.W., McMurtry J.P. and Coon C.N. (2007). Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks. Poult. Sci. 86, 673-683.
Mello M.A. and Tuan R.S. (2006). Effects of TGF‐β1 and triiodothyronine on cartilage maturation: In vitro analysis using long-term high-density micromass cultures of chick embryonic limb mesenchymal cells. J. Orthop. Res. 24, 2095-2105.
Moran E.T. (2007). Nutrition of the developing embryo and hatchling. Poult. Sci. 86, 1043-1049.
Nalon E. and Stevenson P. (2019). Addressing lameness in farmed animals: An urgent need to achieve compliance with EU animal welfare law. Animal. 9, 576-585.
Nys Y. and Le R.N. (2018). Calcium homeostasis and eggshell biomineralization in female chicken. Pp. 361-382 in Vitamin D. D. Feldman, M. Wesley Pike, R. Bouillon, E. Giovannucci, D. Goltzman and J. Hewison, Eds., Academic Press, Cambridge, MA, USA.
Onyango E.M., Hester P.Y., Stroshine R. and Adeola O. (2003). Bone densitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels. Poult. Sci. 82, 1787-1791.
Porter T.E., Couger G.S., Dean C.E. and Hargis B.M. (1995). Ontogeny of growth hormone (GH)-secreting cells during chicken embryonic development: initial somatotrophs are responsive to GH-releasing hormone. Endocrinology. 136, 1850-1856.
Power M.J. and Fottrell P.F. (1991). Osteocalcin: diagnostic methods and clinical applications. Crit. Rev. Clin. Lab. Sci. 28, 287-335.
Roddy K.A., Prendergast P.J. and Murphy P. (2011). Mechanical influences on morphogenesis of the knee joint revealed through morphological, molecular and computational analysis of immobilised embryos. PLoS One. 6, e17526.
SAS Institute. (2002). SAS®/STAT Software, Release 9.0. SAS Institute, Inc., Cary, NC. USA.
Shafey T.M., Al-mohsen T.H., Al-sobayel A.A., Al-hassan M.J. and Ghnnam M.M. (2002). Effects of eggshell pigmentation and egg size on the spectral properties and characteristics of eggshell of meat and layer breeder eggs. Asian-Australasian J. Anim. Sci. 15, 297-302.
Shafey T., Ghannam M., Al-Batshan H.A. and Al-Ayed M.S. (2004). Effect of pigment intensity and region of eggshell on the spectral transmission of light that passes the eggshell of chickens. Int. J. Poult. Sci. 3, 228-233.
Shao Y., Sun G., Cao S., Lu L., Zhang L., Liao X. and Luo X. (2019). Bone phosphorus retention and bone development of broilers at different ages. Poult. Sci. 98, 2114-2121.
Shen G. (2005). The role of type X collagen in facilitating and regulating endochondral ossification of articular cartilage. Orthod. Craniofac. Res. 8, 11-17.
Sindhurakar A. and Bradley N.S. (2012). Light accelerates morphogenesis and acquisition of interlimb stepping in chick embryos. PLoS One. 7, e51348.
Stevens D.A., Hasserjian R.P., Robson H., Siebler T., Shalet S.M. and Williams G.R. (2000). Thyroid hormones regulate hypertrophic chondrocyte differentiation and expression of parathyroid hormone-related peptide and its receptor during endochondral bone formation. J. Bone Miner. Res. 15, 2431-2442.
Tong Q., McGonnell I., Romanini C.E, Bergoug H., Roulston N., Berckmans D., Exadaktylos V., Guinebretiere M., Eterradossi N., Garain P. and Demmers T. (2015). Effect of a photoperiodic green light program during incubation on embryo development and hatch process. Agric. Eng. Int: CIGR J. 2015, 264-267.
Van der Pol C.W., van Roovert-Reijrink I.A.M., Aalbers G., Kemp B. and van den Brand H. (2017). Incubation lighting schedules and their interaction with matched or mismatched post hatch lighting schedules: Effects on broiler bone development and leg health at slaughter age. Res. Vet. Sci. 114, 416-422.
Van der Pol C.W., van Roovert-Reijrink I.A.M., Gussekloo S.W.S., Kranenbarg S., Leon-Kloosterziel K.M., van Eijk-Priester M.H., Zeman M., Kemp B. and van den Brand H. (2019a). Effects of lighting schedule during incubation of broiler chicken embryos on leg bone development at hatch and related physiological characteristics. PLoS One. 14, e0221083.
Van der Pol C.W., van Roovert-Reijrink I.A.M., Maatjens C.M., Gussekloo S.W.S., Kranenbarg S., Wijnen J., Pieters R.P.M., Schipper H., Kemp B. and van den Brand H. (2019b). Light-dark rhythms during incubation of broiler chicken embryos and their effects on embryonic and post hatch leg bone development. PLoS One. 14, e0210886.
Van der Pol C.W., Molenaar R., Buitink C.J., van Roovert-Reijrink I.A.M., Maatjens C.M., van den Brand H. and Kemp B. (2015). Lighting schedule and dimming period in early life: consequences for broiler chicken leg bone development. Poult. Sci. 94, 2980-2988.
Varga F., Spitzer S. and Klaushofer K. (2004). Triiodothyronine (T3) and 1, 25-dihydroxyvitamin D3 (1, 25D3) inversely regulate OPG gene expression in dependence of the osteoblastic phenotype. Calcif. Tissue Int. 74, 382-387.
Willemsen H., Everaert N., Witters A., De Smit L., Debonne M., Verschuere F., Garain P., Berckmans D., Decuypere E. and Bruggeman V. (2008). Critical assessment of chick quality measurements as an indicator of posthatch performance. Poult. Sci. 87, 2358-2366.
Williams B., Solomon S., Waddington D., Thorp B. and Farquharson C. (2000). Skeletal development in the meat-type chicken. Br. Poult. Sci. 41, 141-149.
Yair R., Uni Z. and Shahar R. (2012). Bone characteristics of late-term embryonic and hatchling broilers: Bone development under extreme growth rate. Poult. Sci. 91, 2614-2620.
Ye J., Coulouris G., Zaretskaya I., Cutcutache I., Rozen S. and L Madden T. (2012). Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinform. 13, 134-142.
Yildiz H., Gunes N., Cengiz S., Ozcan R., Petek M., Yilmaz B. and Arican I. (2009). Effects of ascorbic acid and lighting schedule on tibiotarsus strength and bone characteristics in broilers. Arch. Tierz. 52, 432-444.
Yu Y., Li Z., Zhong Z., Jin S., Pan J., Rao X. and Yu Y. (2018). Effect of monochromatic green LED light stimuli during incubation on embryo growth, hatching performance, and hormone levels. Trans. ASABE. 61, 661-669.
Zeman M., Gwinner E., Herichová I., Lamosová D. and Kost'ál L. (1999). Perinatal development of circadian melatonin production in domestic chicks. J. Pineal Res. 26, 28-34.
Zhang L., Wu S., Wang J., Qiao X., Yue H., Yao J., Zhang H. and Qi G. (2014). Changes of plasma growth hormone, insulin-like growth factors-I, thyroid hormones, and testosterone concentrations in embryos and broiler chickens incubated under monochromatic green light. Italian J. Anim. Sci. 13, 3266-3275.
Zhang L., Zhu X.D., Wang X.F., Li J.L., Gao F. and Zhou G.H. (2016). Green light-emitting diodes light stimuli during incu-bation enhances posthatch growth without disrupting normal eye development of broiler embryos and hatchlings. Asian-Australasian J. Anim. Sci. 29, 1562-1568.
