تغییر محتوای فیتوشیمیایی و فعالیت آنتی اکسیدانتی گیاه دارویی کلماتیس اصفهانی (Clematis ispahanica Boiss.) در سه رویشگاه طبیعی استان سمنان
الموضوعات :
محمد مهدی ذوالفقاری
1
,
رضا نادری
2
,
عاطفه امیر احمدی
3
1 - گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران،
2 - گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران،
3 - گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران،
الکلمات المفتاحية: اکوفیزیولوژی, تنش شوری, سنجش‌های بیوشیمیایی, زیستگاه, گیاهان دارویی,
ملخص المقالة :
در این مطالعه پتانسیل آنتی‌اکسیدانتی و خواص فیزیولوژیکی گیاه کلماتیس اصفهانی از رویشگاه‌های مختلف استان سمنان بررسی شد. سه رویشگاه طبیعی این گیاه در استان سمنان (چشمه علی، دیباج و مجن) شناسایی و نمونه برداری از برگ گیاهان صورت گرفت. میزان کلروفیل، کاروتنوئید، آنتوسیانین، پرولین، پروتئین، فنول، فلاونوئید، قندهای احیاکننده، پراکسیدهیدروژن، مالون دی آلدهید و فعالیت آنزیم‌های کاتالاز و گایاکول پراکسیداز اندازه گیری شد. همچنین خواص آنتی اکسیدانتی گیاهان با اسـتفاده از ظرفیـت مهـار رادیکال‌هايDPPH مورد بررسی قرار گرفت. آنالیز خاک هر سه رویشگاه نیز انجام شد. آزمایش به‌صورت طرح کاملاً تصادفی با 3 تکرار و مقایسـه میـانگین داده‌هـا بـا استفاده از آزمون دانکن در سطح احتمال 5 درصد انجام شد. نتایج نشان داد که مقدار متابولیت‌های گیاه شامل قندهای محلول، ترکیبات فنولی و فلاونوئیدها، فعالیت آنزیم کاتالاز و خواص آنتی اکسیدانتی در رویشگاه چشمه علی در بالاترین و در رویشگاه مجن در کمترین میزان قرار دارد. در مقابل مقدار ترکیبات دفاعی گیاه شامل پرولین، پراکسیدهیدروژن، مالون دی آلدهید و فعالیت آنزیم گایاکول پراکسیداز در رویشگاه چشمه علی در کمترین و در رویشگاه مجن در بالاترین میزان قرار دارد. مقدار کلروفیل، کارتنوئیدها، آنتوسیانین و پروتئین نیز در رویشگاه دیباج با بالاترین ارتفاع از سطح دریا در کمترین میزان قرار دارد. نتایج این تحقیق نشان داد که رویشگاه چشمه علی با داشتن بیشترین میزان متابولیت‌ها و کمترین میزان ترکیبات دفاعی کم تنش‌ترین رویشگاه و بالعکس رویشگاه مجن پرتنش‌ترین رویشگاه برای گیاه کلماتیس اصفهانی می‌باشد.
Amirahmadi, A., Naderi, R. and Afsharian, M.H. (2022). An investigation into the medicinal plants of Semnan province with taxonomic and therapeutic aspects. Trends in Phytochemical Research. 6(4), 312-338.
Amiri, S. (2013). Investigating the morphophenological traits, pollination method and the amount of essential oil of Clematis ispahanica under conditions of drought stress and lack of drought stress. Msc thesis, Faculty of Agriculture, Isfahan University of Technology.
Asadi, S.Y., Parsaei, P., Karimi, M., Ezzati, S., Zamiri, A. and Mohammadizadeh, F. (2013). Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat. International Journal of Surgery. 11(4): 332-337.
Asadi-Samani, M., Rafieian-Kopaei, M. and Azimi, N. (2013). Gundelia. A systematic review of medicinal and molecular perspective. Pakistan Journal of Biological Science. 16(21): 1238-1247.
Ashrafzadeh, M., Gharemakher, H.N., Heshmati, G., Saharkhiz, M. and Nohooji, M.G. (2019a). Ecological characteristics and forage quality of Clematis ispahanica in Fars and Yazd Provinces. Iranian Journal of Range and Desert Research. 26(2): 432-446.
Ashrafzadeh, M., Niknahad, H., Saharkhiz, M., ghorbani Nohouji, M. and Heshmati, G. (2019b). Breaking seed dormancy of Clematis ispahanica and its optimum planting depth and density (Case study: Bavanat, Fars Province). Iranian Journal of Range and Desert Research. 26(3), 629-639.
Azizi, K., Naji, H.R., Hassaneian Khoshroo, H. and Mehdi, H.M. (2020). Effect of altitude and growing season on some physiological properties of leaf from Persian oak (Quercus brantii) in Zagros forest (case study: Ilam). Plant Process and Function. 9 (35):101-114.
Bates, L., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-207.
Bhattacharjee, S. and Mukherjee, A.K. (2002). Salt stress induced cytosolute accumulation, antioxidant response and membrane deterioration in three rice cultivars during early germination. Seed Science and Technology. 30: 279-287.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 72(1-2): 248-254.
Campos, P.S., Ramalho, J.C., Lauriano, J.A., Silva, M.J. and do Ceu Matos, M. (1999). Effects of drought on photosynthetic performance and water relations of four Vigna genotypes. Photosynthetica 36: 79-87.
Chang, C., Yang, M., Wen, H. and Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 10: 178-182.
Chawla, R., Kumar, S. and Sharma, A. (2012). The genus Clematis (Ranunculaceae): Chemical and pharmacological perspectives. Journal of Ethnopharmacology. 143(1): 116-150.
Chittoor, J. T., Balaji, L. and Jayaraman, G. (2016). Optimization of parameters that affect the activity of the alkaline protease from halotolerant bacterium, Bacillus acquimaris VITP4, by the application of response surface methodology and evaluation of the storage stability of the enzyme. Iranian Journal of Biotechnology. 14(1): 23-32.
Chohra, D., Ferchichi, L., Cakmak, Y.S., Zengin, G. and Alsheikh, S.M. (2020). Phenolic profiles, antioxidant activities and enzyme inhibitory effects of an Algerian medicinal plant (Clematis cirrhosa L.). South African Journal of Botany. 132: 164-170.
Dinarvan, M. (2020). Clematis khuzestanica (Ranunculaceae) a new species from southwest of Iran. Iranian Journal of Botany. 26(1): 16-18.
Eisapoor, M., Hemmati, K. and Hemmati, N. (2020). Study of the effect of habitat on morphological and phytochemical traits of horsemint (Mentha longifolia L.). Journal of Horticultural Science. 33(4): 698-710.
Farhoudi, R. (2011). Evolution effect of salt stress on growth, antioxidant enzymes activity and malondealdehaid concentration of Canola verities. Iranian Journal of Field Crops Research. 9(1): 123-130.
Farzaneh, M., Amirahmadi, A., Poozesh, V. and Salimi, F. (2021). Study on Phytochemical diversity and antioxidant properties of extracts from different populations of Perovskia abrotanoides Kar. in Eastern Alborz. Eco-phytochemical Journal of Medicinal Plants. 9(3): 16-28.
Fathi, T., Seghatoleslami, M., Yari, R. and Nakhaei, F. (2020). Study on some ecomorphological, phenological, and phytochemical characteristics of common mallow (Malva sylvestris L.) in two native habitats of Ferdows and Tabas regions. Iranian Journal of Medicinal and Aromatic Plants Research. 36(4): 590-608.
Fathi, T., Seghatoleslami, M.J., Yari, R. and Nakhaei, F. (2020). Study on some ecomorphological, phenological, and phytochemical characteristics of common mallow (Malva sylvestris L.) in two native habitats of Ferdows and Tabas regions. Iranian Journal of Medicinal and Aromatic Plants. 36(4): 590-608.
Feder, M.E. and Hofmann, G.E. (1999). Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annual review of physiology. 61(1): 243-282.
Fedina, I.S., Georgieva, K. and Grigorova, I. (2002). Light-dark changes in proline content of barley leaves under salt stress. Plant Biology. 45: 59-63.
Firoozeh, R., Khavarinejad, R., Najafi, F. and Saadatmand, S. (2019). Effects of gibberellin on contents of photosynthetic pigments, proline, phenol and flavonoid in savory plants (Satureja hortensis L.) under salt stress. Journal of Plant Research (Iranian Journal of Biology). 31(4): 894-908.
Foy, C.D., Chaney, R.L. and White, M.C. (1978). The physiology of metal toxicity in plants. Annual Review of Plant Physiology. 29: 511-566.
Gairola, S., Shariff, N.M., Bhatt, A. and Kala, C.P. (2010). Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. Journal of Medicinal Plants Research. 4(18): 1825-1829.
Ghasemi Pirbalouti, A. (2010). Medicinal and Aromatic Plants (Identifying and examining their effects). Shahrekord, Iran: Publisher of I.A.U. Shahrekord Branch.
Ghorbanli, M. Satei, A. and Maghise, A. (2003). The effect of different amounts of salinity on the activities of catalase, peroxidase and nitrate reductase enzymes in the roots and leaves of Canola cultivars. Pajouhesh-va-Sazandegi. 58: 39-43.
Guo, Z., Ou, W., Lu, S. and Zhong, Q. (2006). Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry. 44: 828-836.
Hameed, A., Naseer, S., Iqbal, T., Syed, H. and Haq, M.A. (2008). Effects of NaCl salinity on seedling growth, senescence, catalase and protease activities in two wheat genotypes differing in salt tolerance. Pakistan Journal of Botany. 40(3): 1043-1051.
Handa, S.S., Rakesh, D.D. and Vasisht, K. (2006). Compendium of Medicinal and Aromatic Plants Asia. Italy: ICS UNIDO.
Hare, P.D. and Cress, W. A. (1997). Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulation. 21: 79-102.
Heath, R.L. and Packer, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125(1): 189-198.
Heydari, M., Mesri, F. and Keykha, Z. (2010). Effects of salinity stress on nucleic acid metabolism, antioxidants enzyme activity, chlorophyll fluorescence and osmotic adjustment in five Canola genotypes. Iranian Journal of Field Crop Science. 41(3): 491-502.
Hipskind, J., Wood, K. and Nicholson, R.L. (1996). Localized stimulation of anthocyanin accumulation and delineation of pathogen ingress in maize genetically resistant to Bipolaris maydis race O. Physiological and Molecular Plant Pathology. 49(4): 247-256.
Iranshahr, M., Rechinger, K.H. and Riedl, H. (1992). Ranunculaceae. In: Flora Iranica, vol. 171. ed. K.H. Rechinger. Graz: Akademische Druck- und Verlagsanstalt.
Kaghazloo, Z., Hemati, K. and Khorasaninejad, S. (2017). The effect of height on some secondary metabolites of different organs of Sambucus (Sambucus ebulus L.) in three cities of Golestan province. Journal of Iranian Plant Ecophysiological Research. 12(47): 31-43.
Karimi, E., Ghorbani Nohooji, M., Habibi, M., Ebrahimi, M., Mehrafarin, A. and Khalighi-Sigaroodi, F. (2018). Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae). Natural Product Research. 32(16): 1991-1995.
Koleva, I.I., van Beek, T.A., Linssen, J.P.H., de Groot, A. and Evstatieva, L.N. (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis. 13: 8-17.
Koohpayeh, A., Ghasemi Pirbalouti, A., Yazdanpanah Ravari, M.M., Pourmohseni Nasab, E. and Arjomand, D. (2011). Study the ethno-veterinary of medicinal plants in Kerman province, Iran. Journal of Medicinal Herbs. 2(3): 211-216. (in Persian).
Kumaran, A. and Karunakaran, R.J. (2007). In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Food Science and Technology. 40(2): 344-352.
Kuznetsov, V. and Shevyakova, N.I. (1999). Proline under stress, biological role, metabolism and regulation. Russian Journal of Plant Physiology. 46: 274-287.
Lee, H.S. (2000). The experimental principle and technology of plant physiology and biochemistry. Beijing: Higher Education Press.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in enzymology. 148: 350-382.
Maehly, A.C. and Chance, B. (1954). The assay of catalase and peroxidase. In: Methods in biochemistry analysis, vol 1., pp. 357-425. ed. D. Glick. New York: Interscience Publishers.
Mohseni, Z., Moradian, F. and Rahdari, P. (2020). The study of activity of antioxidant enzymes, guaiacol peroxidase and ascorbate peroxidase and the amount of Na, K and pigment content in Spinach oleracea L. under NaCl salinity stress. Journal of Plant Research (Iranian Journal of Biology). 32(4): 915-924.
Mojarab, S., Moghaddam, M. and Narimani, R. (2018). The effect of pretreatment of salicylic acid on seed germination, total phenol and antioxidant activity of Nepeta nuda L. seedling under salt stress. Eco-phytochemical Journal of Medicinal Plants. 6(1): 21-31.
Nabati, J., Kafi, M., Nezami, A., Rezvani Moghaddam, P., Masoumi, A. and Zare Mehrjerdi, M. (2014). Evaluation of quantitative and qualitative characteristic of forage kochia (Kochia scoparia) in different salinity levels and time. Iranian Journal of Field Crops Research. 12(4): 613-620.
Nabavi, S.J., Zali, S.H., Ghorbani, J. and Kazemi, S.Y. (2018). Effects of some ecological factors on extract of Juniperus communis L. in Mountainous Rangelands of Hezarjarib - Behshahr. Plant Process and Function. 6(19): 367-374.
Naderi, R., Zakeri, A. and Afsharian, M.H. (2021). Identification of medicinal plants of Semnan province: description, distribution and therapeutic properties. Damghan: Damghan University Publisher.
Papari Moghadam Fard, M., Ketabchi S. and Farjam, M.H. (2020). Chemical composition, antimicrobial and antioxidant potential of essential oil of Ziziphus spina-christ var aucheri grown wild in Iran. Journal of Medicinal Plants and By-product. 9(Special): 69-73.
Parvizi, A., Hatamnia, A.A., Mohammadkhani, N. and Naji, H.R. (2021). Effect of altitude on photosynthesis rate and some physiological indices from three species of Quercuis brantii, Pistacia atlantica, Crataegus pontica in Ilam province forests. Plant Process and Function. 10(45): 57-70.
Parvizi, A., Hatamnia, A.A., Mohammadkhani, N. and Naji, H.R. (2021). Effect of altitude on photosynthesis rate and some physiological indices from three species of Quercus brantii, Pistacia atlantica, Crataegus pontica in Ilam province forests. Plant Process and Function. 10 (45): 57-70.
Pezhmanmehr, M., Ebadi, A., Moosavi, A. R., Walker, A. and Rahimi, A. (2015). Quantitative and qualitative analysis of anthocyanins and flavonols in berry skin of some grape cultivars using High Performance Liquid Chromatography (HPLC). Journal of Medicinal Plants. 14 (56): 123-138.
Prior, R.L. and Cao, G. (2000). Analysis of botanicals and dietary supplements for antioxidant capacity: a review. Journal of AOAC International. 83(4): 950-956.
Raei, F., Ghorbani Nohooji, M., Habibi, M. and Ashoori, N. (2014). Antibacterial activity of alcoholic extracts of two Clematis L. (Ranunculaceae) Species from Iran. Journal of Medicinal Plants, 13(49): 39-45.
Razmjoo, K., Khodaeian, N., Razzazi, A. and Askari, E. (2009). Breaking seed dormancy of Clematis ispahanica Bioss. a medicinal plant of Iran. Seed Technology. 31(1): 101-107.
Saberi, M., Niknahad, H., Heshmati, G.A., Barani, H. and Shahriari, A.R. (2018). Evaluation of the content and performance of some active ingredients extracts of Citrullus colocynthis organs from two habitats of Sistan and Balochestan province in different growth stages. Journal of Plant Ecosystem Conservation. 5 (11): 49-63.
Schwambach, J., Ruedell, C.M., de Almeida, M.R., Penchel, R.M., de Araújo, E.F. and Fett-Neto, A.G. (2008). Adventitious rooting of Eucalyptus globulus × maidennii minicuttings derived from mini-stumps grown in sand bed and intermittent flooding trays: a comparative study. New Forests. 36(3): 261-271.
Shariat, A. and Asareh, M.H. (2008). Effects of drought stress on pigments, prolin, soluble sugar and growth parameters on four Eucalyptus species. Pajouhesh-va-Sazandegi. 87: 139-148.
Simova-Stoilova, L., Demirevska, K., Petrova, T., Tsenov, N. and Feller, U. (2008). Antioxidative protection in wheat varieties under severe recoverable drought at seedling stag. CAAS Agricultural Journals. 54(12): 529-536.
Sofo, A., Scopa, A., Nuzzaci, M. and Vitti, A. (2015). Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. International Journal of Molecular Sciences. 16: 13561-13578.
Somogy, M. (1952). Note on sugar determination. Journal of Biological Chemistry. 195: 19-29.
Stewart, C.R. and Voetberg, G. (1985). Relationship between stress-induced ABA and proline accumulations in excised barley leaves. Plant Physiology. 79: 24-27.
Torabi Pashai, S., Niknam, V., Ebrahimzadeh, H. and Sharifi, G.A. (2017). Comparative study of biochemical responses of different saffron (Crocus sativus) accessions to salt stress and alleviative effects of salicylic acid. Journal of Plant Research (Iranian Journal of Biology). 29(4): 728-740.
Turner, N.C. (1997). Further progress in crop water relations. Advances in Agronomy. 58: 293-338.
Upadhyaya, A., Sankhla, D., Davis, T.D., Sankhla, N. and Smith, B. (1985). Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. Journal of Plant Physiology. 121(5): 453-61.
Wagner, G.J. (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant physiology. 64(1): 88-93.
Younesi-Melerdi, E., Nematzadeh, G.A. and Pakdin Parizi, A. (2019). Antioxidant responses of different tissues of Aeluropus littoralis to salinity stress. Journal of Plant Process and Function. 8(32): 433-446.
Zakeri, A., Naderi, R. and Poozesh, V. (2020). An investigation of plant species distribution in Semnan province (Case study: Herbarium of Damghan University). Journal of Plant Researches. 33(4):817-827.
