Acute Toxicity, Brine Shrimp Lethality and Phytochemical Screening of Lannea schimperi and Searsia longipes
الموضوعات :Musa Chacha 1 , Nicolaus Mbugi 2
1 - Nelson Mandela African Institution of Science and Technology, Tanzania
2 - Nelson Mandela African Institution of Science and Technology, Tanzania
الکلمات المفتاحية: Phytochemical screening, Acute toxicity, Cytotoxicity, Brine shrimp, Searsia longipe, , Lannea schimperi,
ملخص المقالة :
Lannea schimperi and Searsia longipes are plants species under family Anacardiaceae. These plants have been utilized for years in traditional settings for management of an array of disease conditions; however, there is limited information about their safety (toxicity level). The aim of the current study was to evaluate the acute toxicity, cytotoxicity and phytochemical compounds of extracts from the aforementioned plants. Acute toxicity was performed in vivo on Swiss albino mice. Cytotoxicity was done in vitro on brine shrimp larvae and plants were qualitatively screened for five major groups of compounds. Both extracts exhibited good margin of safety on swiss albino mice with LD50 (Lethal dose 50) above 2000 mg/kg body weight. Lannea schimperi and Searsia longipes expressed significant cytotoxicity on brine shrimp larvae with IC50 (Inhibitory concentration 50) of 150.0478 mg/ml and 280.7875 mg/ml respectively. Phytochemical screenings of both extracts have revealed presence of flavonoids, saponnins, tannins and glycosides. The study confirmed the previous reports on the acute toxicity of Lannea schimperi and Searsia longipes, as well as cytotoxicity of Lannea schimperi and for the first time reports the cytotoxicity and phytochemical compounds of Searsia longipes.
1. Maroyi A., 2011. An Ethnobotanical Survey of Medicinal Plants Used by the People in Nhema Communal Area, Zimbabwe. J. Ethnopharmacol. 136(2), 347–54. Retrieved (http : // dx.doi.org / 10.1016 / j. jep. 2011 . 05 . 003)
2. Okoth D.A., 2014. Phytochemistry and Bioactive Natural Products from Lannea Alata, Lannea rivae, Lannea schimperi and Lannea schweinfurthii (Anacardiaceae ). Phd Thesis, University of Kwazulu-Natal, South Africa.
3. Moshi M.J., Mbwambo Z.H., 2002. Experience of Tanzanian Traditional Healers in the Management of Non-Insulin Dependent Diabetes Mellitus. Pharm Biol. 40(7), 552–560.
4. Kisangau D.P., Lyaruu H.V., Hosea K.M., Joseph C.C., 2007. Use of Traditional Medicines in the Management of HIV/AIDS Opportunistic Infections in Tanzania: A Case in the Bukoba Rural District. J Ethnobiol Ethnomed. 3,1-8.
5. Chinsembu K.C., Hedimbi M., 2010. An Ethnobotanical Survey of Plants Used to Manage HIV/AIDS Opportunistic Infections in Katima Mulilo, Caprivi Region, Namibia. J Ethnobiol Ethnomed. 6, 1-9.
6. Moshi M.J., Mbwambo Z.A., Nondo R.S., Masimbaa P.J., Kamuhabwa A., Kapingu M. C., Thomas P., Richard M., 2006. Evaluation of Ethnomedical Claims and Brine Shrimp Toxicity of Some Plants Used in Tanzania as Traditional Medicines. African J. Tradit Complement. Altern Med. 3(3), 48-58.
7. Jeruto P., Lukhoba C., Ouma G., Otieno D., Mutai C., 2008. An Ethnobotanical Study of Medicinal Plants Used by the Nandi People in Kenya. J Ethnopharmacol. 116(2), 370-376.
8. Egbe E.O., Akumka D.D., Adamu M., Mikail H.G., 2015. Phytochemistry, Antinociceptive and Anti-inflammatory Actvities of Methanolic Leaves Extract of Lannea schimperi (Hoschst. Ex Rich) ENG. Recent Pat. Biotechnol. 9(2), 145-152.
9. Sherfi T.K., Ibrahim A.A., Ahmed A.A.A., Omer G., 2016. Phyto-Constituents and Antioxidant Activity of Rhus abyssinica, Heeria insignis and Lannea schimperi (Anacardiaceae family). Eur Acad Res. 4(8), 6493 - 6509.
10. Haule E.E., Moshi M.J., Nondo R.S., Mwangomo D.T., Mahunnah R.L., 2012. A study of antimicrobial activity, acute toxicity and cytoprotective effect of a polyherbal extract in a rat ethanol-HCl gastric ulcer model. BMC Res Notes. 5(1), 1-9.
11. Djakpo O., Yao W., 2010. Rhus Chinensis and Galla Chinensis – Folklore to Modern Evidence: Review Phytother Res. 24, 1739–1747.
12. Miller A.J., Young D.A., Wen J., 2001. Phylogeny and Biogeography of Rhus (Anacardiaceae ) Based on ITS Sequence Data. Int J Plant Sci. 162(6), 1401–1407.
13. Duke J.A., Ayensu E.S., 1985. Medicinal Plants of China. Vol. 2. Algonac, MI.
14. Kuo S.C., Teng C.M., Lee L.G., Chiu T.H., Wu T.S., Huang S.C., Wu J.B., Shieh T.Y., Chang R.J., Chou T. C., 1990. 6-Pentadecylsalicylic Acid; An Antithrombin Component Isolated from the Stem of Rhus semialata var. roxburghii. Planta Med. 57(3), 247-249.
15. Ouyang M.A., Chang C.I., Wein Y.S., Kuo Y.H., 2008. New Phenol Glycosides from the Roots of Rhus Javanica Var. Roxburghiana. J Chin Chem. Soc. 55(1), 223-227.
16. Abbasi A.M., Khan M.A., Ahmad M., Zafar M., Khan H., Muhammad N., Sultana S., 2009. Medicinal Plants Used for the Treatment of Jaundice and Hepatitis Based on Socio-Economic Documentation. Afr J Biotechnol. 8(8), 1643–1650.
17. Abbassi F., Hani K., 2012. In vitro antibacterial and antifungal activities of Rhus tripartitum used as antidiarrhoeal in Tunisian folk medicine. Nat Prod Res. 26(23), 2215-2218.
18. Kuma F., Birhanu T., Hirpa E., Nekemte E., 2015. Advanced Review on Anthelmintic Medicinal Plants. Rep Opin. 7(5), 6-16.
19. Tlili N., Mejri H., Yahia Y., Saadaoui E., Rejeb S., Khaldi A., Nasri N., 2014. Phytochemicals and Antioxidant Activities of Rhus Tripartitum (Ucria) Fruits Depending on Locality and Different Stages of Maturity. Food Chem. 160, 98–103.
20. Mtunzi F.M., Ejidike I.P., Matamela T., Dikio E., Klink M.J., 2017. Phytochemical Profiling , Antioxidant and Antibacterial Activities of Leaf Extracts from Rhus Leptodictya. International Journal of Pharmacognosy and Phytochemical Research. Int J Phytochem Pharmacol Res. 9(8), 1090–1099.
21. Anwer T., Sharma M., Khan G., Iqbal M., Ali M.S., Alam M.S., Safhi M.M., Gupta N., 2013. Rhus coriaria ameliorates insulin resistance in non-insulin-dependent diabetes mellitus (NIDDM) rats.
Acta Pol Pharm. 70(5), 861-867.
22. Abu-Reidah I.M., Ali-Shtayeh M.S., Jamous R.M., Arráez-Román D., Segura-Carretero A., 2015. HPLC-DAD-ESI-MS/MS Screening of Bioactive Components from Rhus Coriaria L. (Sumac) Fruits. Food Chem.166, 179–191.
23. OECD., 2001. Guidance Document on Acute Oral Toxicity Testing. OECD Guidel. Test. Chem.
24. Meyer B.N., Ferrigni N.R., Putnam J.E., Jacobsen L.B., Nichols D.E., McLaughlin J.L., 1982. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Med. 45(5), 31-34.
25. Gul R., Jan S.U., Faridullah S., Sherani S., Jahan N., 2017. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. Sci World J. 2017, 1-7.
26. Olorunnisola O. S., Adetutu A., Owoade A. O., Adesina B. T., Adegbola P., 2017. Toxicity evaluation and protective effect of Rhus longipes Engl. leaf extract in paracetamol induced oxidative stress in wister rats. J Phytopharmacol. 6(2),73–77.
27. Diallo A., Eklu-Gadegkeku K., Mobio T., Moukha S., Agbonon A., Aklikokou K., Creppy E.E., Gbeassor M., 2009. Protective effect of Moringa oleifera Lam. and Lannea kerstingii extracts against cadmium and ethanol-induced lipid peroxidation. J Pharmacol Toxicol.4(4), 160-166.
28. Sanchez C., Gupta M., Vasquez M., Montenegro G., 1993. Bioessay with Brine Artemia to Predict Antibacterial and Pharmacologic Activity. Rev Med Panama. 18(1), 62-69.
29. Gabr S.A., Alghadir A.H., 2015. Phytochemical analysis and in vitro antifungal activities of bioactive fractions from leaves of Rhus coriaria (SUMAC). J Pure Appl Microbiol. 9, 559-565.
