UV-Visible spectrophotometry of Repaglinide in bulk and in formulation by using methyl orange as reagents
الموضوعات : Journal of the Iranian Chemical ResearchSmita Sharma 1 , Mukesh Sharma 2
1 - Department of Chemistry Chodhary Dilip Singh Kanya Mahavidyalya Bhind (M.P) 477001, India
2 - School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore (M.P)-452001, India
الکلمات المفتاحية: Repaglinide, Derivative spectrophotometry, Area under curve,
ملخص المقالة :
Two sensitive spectrophotometric methods are presented for the assay of Repaglinide in bulkdrug and in formulations using methyl orange, as reagents. Oxidant by reacting with a fixedamount of either methyl orange and measuring the absorbance at 485.2 nm (method-I) andmeasuring the absorbance at 618.9 nm (method-II). Developed method is based on the formationof extractable colored complex of drug with coloring agent Methyl orange dye. A wavelengthmaximum was found to be 618.9 nm. The concentration range of 15-50 μg ml-1 with linearregression of 0.9995, while the percentage recovery, LOD and LOQ were 99.42-99.08 %, 2.17μg ml-1 and 1.08 μg ml-1 respectively. The result of analysis have been validated statistically andalso by recovery studies. From the percentage recovery and specificity studies it was concludedthat there was no interference of common additives during the estimation. This proves thesuitability of this method for the routine quality control analysis of the Repaglinide informulation.
[1] M.D.Rockville, United States Pharmacopoeia; 25th (Eds) United States Pharmacopoeial Convention
Inc, (2002) 2150.
[2] S.Budavari, the Merck Index. 13th (eds) Whitehouse Station (NJ, USA) Merck and Co Inc (2001) 790.
[3] J.E.F Reynolds, Martindale, 33 rd ed. (London), the Complete Drug Reference Pharmaceutical Press,
(2002) 334.
[4] M.D. Rockville, United State Pharmacopoeia, (Asian Eds) USP Convention Inc, (2003) 623.
[5] A.B. Ruzilawati, M.S. Wahab, A. Imran, Z. Ismail, S.H. Gan, J. Pharm. Biomed. Anal. 43 (2007)
1831-1835.
[6] M. Gandhimathi, T.K. Ravi, S.K. Renu, Anal. Sci. 19 (2002) 1675-1677.
[7] R.H. Khan, S. Talegaonkar, R.M. Singh, S.C. Mathur, R. Shiv, G.N. Singh, Indian Drugs 44 (2007)
428-433.
[8] A. Gumieniczek, A. Berecka, H. Hopkała, J. Planar Chromatogr. 18 (2005) 155-159.
Parameters Repaglinide
Method I Method II
Working λ (nm) 485.2 nm
618.9 nm
Beer’s law limit (μg mL-1) 5-35 5-35 5-35 0-45
Correlation coefficient * 0.9965 0.9994 0.9998 0.9999
Intercept * 0.0003 0.0014 0.0043 0.0021
Slope * 0.0143 0.0221 0.0651 0.0765
Limit of detection, μg mL-1 2.17 -0.74
Limit of quantification, μg mL-1 1.08 0.18
S. Sharma & M.Ch. Sharma / J. Iran. Chem. Res. 4 (2011) 69-76
76
[9] A. Goyal, I. Singhvi, Indian. J. Pharm. Sci. 68 (2006) 656-657.
[10] ICH, Stability Testing of New Drug Substances and Products, in: Proceeding of the International
Conference on Harmonisation, IFPMA, Geneva, (2003).
[11] M. Bakshi, S. Singh, J. Pharm. Biomed. Anal. 28 (2003) 1011-1040.
[12] A.B. Thomas, M.R. Patankar, K.R. Deshmukh, L.P. Kothapalli, S.J. Jangam, S.H. Bodkhe, A.D.
Deshpande, Indian Drugs 44 (2007) 745-750.
[13] S.B. Chepurwar, A.A. Shirkhedkar, S.B. Bari, S.J. Surana, S.J. Indian Drugs 43 (2006) 803-806
[14] A.G. Davison, A.H. Beckette, J.B. Stenlake, Practical Pharmaceutical Chemistry, CBS Publishers
and distributors, New Delhi.275 (1997).
