Effects of Seed Priming on Morph-physiological Traits of Three Ecotypes of Astragalus squarrosus Bunge Grown in Iran
الموضوعات :Mahshid Souri 1 , Saeedeh Nateghi 2 , Nadia Kamali 3 , Mina Bayat 4
1 - Assistant Professor, Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2 - Assistant Professor, Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
3 - Assistant Professor, Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
4 - Senior Research Expert, Rangeland Research Division, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
الکلمات المفتاحية: Vegetation cover, Seed, Plant height, Proline, Carbohydrate, Astragalus squarrosus,
ملخص المقالة :
Astragalus squarrosus Bunge is one of the native and compatible range species in the dry lands of Iran. It is of great importance in terms of tolerance to specific ecological conditions as well as high nutritional value and soil conservation. In order to study the effects of seed priming on some morph-physiological traits, an experiment was conducted in form of split plots based on a completely randomized block design with five replications in Torud Research Farm in Semnan Province, Iran in 2017. Seed samples were collected from their natural habitats in Yazd, Kashan and Semnan in Iran. Seeds of three A. squarrosus ecotypes were treated by hydropriming (distilled water), hormonal priming (gibberellic acid: with concentrations of 125 and 250 ppm), (Salicylic acid: 100 and 200 mg/l), (Ascorbic acid: 100 and 200 mM) osmopriming (potassium nitrate: 0.3 and 0.2%) and control (without priming). The primed seeds and controls were sown in the field. No seeds were germinated for control and gibberellic acid (250 ppm). The results showed that the effect of priming was significant on leaf carbohydrate content, vegetation cover, dry matter (DM), yield and plant height (p<0.01). According to the results, the highest DM yield and plant height with average values of 82 (g/m2) and 34 cm, respectively, were related to the ecotype of Yazd treated with salicylic acid 100 mg/l. The highest vegetation cover with an average value of 34.6% was observed in Kashan ecotype by applying salicylic acid 100 mg/l. For carbohydrate content, the highest value of 36 (g/100gFW) was obtained in Semnan ecotype using 100 mg/l salicylic acid. The highest rate of proline with a value of 3.39 (Mm/gFW) was obtained in Semnan ecotype using 125 ppm gibberellic acid. It was concluded that application of hormonal seed priming of A. squarrosus could be useful for direct seeding of this species for rehabilitation of degraded arid rangelands of Iran.
Abbasi Khalaki, M., Ghorbani, A. and Samadi Khanghah, S., 2017. The effect of osmopriming and hormo priyming on germination and early growth of Festuca ovina under drought stress (in vitro). Journal of Range and Watershed Management, 70(4):909-913. (In Persian).
Alamri, S. A., Siddiqui, M. H., Al-Khaishani, M.Y. and Ali, H. M., 2018. Response of salicylic acid on seed germination and physio-biochemical changes of wheat under salt stress. Journal of Acta Scientific Agriculture, 2(5): 36-42.
Alias, N.S.B., Billa, L., Muhammad, A. and Singh, A., 2018. Priming and temperature effects on germination and early seedling growth of some Brassica sp. Journal of Acta Hortic, 1225: 407-414.
Alizadeh, M. A. and Nasiri, M., 2012. Seed technology with emphasis on rangeland plant species. Seed and Plant Registration and Certification Institute Publication, Karaj, Iran, P. 197. (In Persian).
Alvani, F., Dianati Tilaki, G. H. and Sadati, E., 2017. The effects of priming with acid ascorbic on physiological traits of Taverniera cuneifolia seeds under drought stress. Journal of Rangeland, 11(3):294-305. (In Persian)
Araghi Shahri, S.M., Dianati Tilaki, G.A., Behtari, B. and Alizadeh, A., 2014. Comparative effects of hydropriming and halopriming on germination performance of Secale montanum Guss, under salinity stress. Journal of Rangeland Science, 4(3):216-223. (In Persian)
Batoli, H., 2008. Ecological study of Astragalus squarrosus Bunge in Kashan sand dunes, Journal of Research and Construction, 21(3):8 - 17.
Bradford, K. J., 1986. Manipulation of seed, water relations via osmotic priming to improve germination under stress conditions. Journal of HortScience, 21 (5): 1105-1111.
Chen, L., Wang, W. S., Wang, T., Meng, X. F., Chen, T. T. and Huang, X. X., 2019. Methyl salicylate glucosylation regulates plant defense signaling and systemic acquired resistance. Journal of plant physiology, 180: 2167–2181.
El-tayeb, M. A., 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Journal of Plant Growth Regulation, 45: 215-224.
Fakhireh, S. and Shahriari, A., 2018. Analysis of seed germination characteristics of Cynodon dactylon affected by treatments of salicylic acid, gibberellic acid and potassium nitrate treatments. Journal of Plant Research, 31(1):166-174. (In Persian).
Farooq, M., Hussain, M., Imran, M., Ahmad, I., Atif, M. and Alghamdi, S. S., 2019. Improving the productivity and profitability of late sown chickpea by seed priming. International Journal Plant Production, 13: 129–139.
Ghasriani, F., Bayat, M., Abolghasemi, M., Abarsaji, G.H., Akbarpour, H., Azami, A., bagheri, H., Bani Asadi, M., Hasan zadeh, M., khodagholi, M., Dehghani Tafti, M A., Rahmani, G.H., Zarekia, S., Zare,M T., zadbar, M., Sharifi Yazdi, M., sharifi, J., Farmahini Farahani, A. and Mohammadpour, M., 2017. Allowable use of the dominant species in steppe and desert rangelands of Iran. Research Institute of Forests and Rangelands publication, Tehran, Iran (In Persian).
Irigoyen, J.J., Emerich, D.W., Sanchez-Diaz, M., 1992. Water stress induces changes in concentration of proline and total soluble sugar in nodulated alfalfa (Medicgo sativa) plants. Journal of Physiologia Plantarum, 84:55-60.
Javadi, T., Arzani, K. and Ebrahimizadeh, H., 2004. The amount of soluble carbohydrates and proline in nine ecotypes of (Pyrus serotina) under drought stress. Journal of Biology, 17 (4): 369-387. (In Persian).
Jisha, K.C. and Puthur, J.T., 2018. Seed hydropriming enhances osmotic stress tolerance potential in Vigna radiata. Journal of Agricultural Research, 7:145–151.
Kang, H. M. and Saltveit, M. E., 2002. Chilling tolerance of maize, cucumber and rice seed ling’s leaves and roots are differently affected by salicylic acid. Journal of Plantarum, 115: 571-576.
Kaur, S., Gupta, A. K. and Kaur, N., 2002. Effect of osmo and hydropriming of chickpea seeds on the performance of crop in the field. Chickpea Pigeonpea News, 9: 15-17.
Kaur, S., Gupta, A. K. and Kaur, N., 2005. Seed priming increases crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy, Crop Science, 91:81-87.
Kavandi, A., Jafari, A A. and Jafarzadeh, M., 2018. Effect of seed priming on enhancement of seed germination and seedling growth of annual sainfoin (Onobrychis crista-galli (L.) Lam.) in medium and long-term collections of Gene Bank. Journal of Rangeland Science, 8(2): 117-128. (In Persian)
Langeroodi, A.R.S. and Noora, R., 2017. Seed priming improves the germination and field performance of soybean under drought stress. Journal of Animal and Plant Sciences, 27(5):1611–1620.
Lock, J.M. and Simpson, K., 1991. Legumes of west Asia, a checklist. Royal Botanic Gardens, Kew. Chicago Press book.
Maassoumi, A. A., 1998. Astragalus in the old world. Check list. Research Inistitute of Forests and Rangelands, Tehran.
Metwally, A., Finkmemeier, I., Georgi, M. and Dietz, K.J., 2003. Salicylicacid alleviates the cadmium Toxicity in barley seedlings. Journal of Plant physiology, 132: 272-281.
Michalska-Klimczak, B., Wyszy ´nski, Z., Paˇcuta, V., Rašovský, M. and Ró˙za ´nska, A., 2018. The effect of seed priming on field emergence and root yield of sugar beet. Journal of Plant, Soil Environment, 64:227–232.
Mir-Mahmoodi, T., Karbalaye Golizadeh, S., Khaliliqhdam, N. and Yazdanseta, S., 2014. The effect of salicylic acid on rate germination and seedling establishment on rapeseed (Brassica napus L.). International Journal of Agriculture Innovations and Research, 2(6): 2319-1473.
Moravcová, Š., Tůma, J., Dučaiová, Z.K, Waligórski, P., Kula, M., Saja, D., Słomka, A., Bąba, W. and Libik-Konieczny, M., 2018. Influence of salicylic acid pretreatment on seeds germination and some defence mechanisms of Zea mays plants under copper stress. Journal of Plant Physiology and Biochemistry, 122:19-30.
Moreno, C. and Seal, C. E., 2018. Papenbrock, J., Seed priming improves germination in saline conditions for Chenopodium quinoa and Amaranthus caudatus. Journal of Agronomy and Crop Science, 204: 40–48.
Musa, A. M., Johansen, J. and Kumar, J., 2001. Short duration chickpea to replace fallow after Aman Rice: the role of on-farm seed priming in the high Briand Tract of Bangladesh. Experimental Agriculture, 37: 509-521.
Muzaffar, W.; Ahmad, R.; Hussain, M.; Farooq, M. and Wakeel, A., 2019. Influence of seed priming and sowing methods on growth and productivity of wheat cultivars differing in seed size in rice-wheat cropping system of Punjab, Pakistan. International Journal of Agriculture and Biology, 21:803–809.
Nasiri, M., 1994. Factor affecting dormancy, germination and seed development. Research Institute of Forests and Rangelands press, pp.63. (In Persian)
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G. A., Sajid, M., Subtain, M. and Shabbir, I., 2013. Seed priming a technique. International Journal of Agriculture and Crop Sciences, 6(20): 1373-1381.
Nourzad, S., Ahmadian, A. and Moghadam, M., 2015. Evaluation of proline chlorophyll carbohydrate index and nutrient uptake in coriander under drought stress and fertilizer treatment. Journal of Crop Research, 13(1): 131-135.
Paquin, R. and Lechasseur, P., 1979. Observations sur une methode dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany, 57:1851-1854.
Podlech, D., 1998. Phylogeny and progression of characters in Old World Astragali (Leguminosae)., In: Zhang A.,Wu S. (Eds.) Floristic characteristics and Diversity of East Asian Plants., China higher Education Press., Beijing., Pp: 405-407.
Rashid, A., Harris, D., Hollington, P. A. and Rafiq, M., 2004. Improving the yield of mungbean (vigna radiata) in the North West frontier province of Pakistan using on-farm seed priming. Experimental Agriculture Journal, 40: 233-244.
Senaratna, T., 2003. Acetyl salicylicacid (Aspirin) and salicylic acid induced multiplestres tolerance in bean and tomato plant. Plant Growth Regulation, 30:157-161.
Shakirova, F. M. and Sahabutdinova, D. R., 2003. Changes in the hormonal status of wheat seed lings induced by salicylic acid and salinity. Journal of Plant science, 164: 317-322.
Shirini, R and Moameri, M., 2018. Effect of gibberellic acid treatments and wet cooling pretreatment on Trifolium pretense. 7th National Conference on Rangeland and Rangeland Management of Iran. Tehran, Iran.
Shoghian, S.H. and Roozbehani, A., 2017. Effect of salicylic acid foliar application on morphological and physiological traits of yield and yield components of phaseolus vulgaris under drought stress, Journal of Crop Physiology, 34(9): 131-146. (In Persian)
Singh, R., Dahiru, Musa, M. and Sani Haliru, B., 2014. Effect of osmopriming duration on germination, emergence, and early growth of Cowpea (Vigna unguiculata (L.) Walp.) in the Sudan Savanna of Nigeria. International Journal of Agronomy. P: 1-4. https://doi.org/10.1155/2014/841238.
Tasgin, E., Atic, O. and Nalbantoglu, B., 2003. Effect of salicylic acid on freezing tolerance in winter wheat leaves. Plant Growth Regul, 41: 231-236.
Torrens-Spence, M. P., Bobokalonova, A., Carballo, V., Glinkerman, C. M., Pluskal, T. and Shen, A., 2019. PBS3 and EPS1 complete salicylic acid biosynthesis from isochorismate in Arabidopsis. Mol. Plant 12:1577–1586.
