افزایش سطح بیان ژن RAGE سودوموناس آئروژینوسا در افراد مبتلا به عفونت های خونی باکتریایی
الموضوعات :
فاطمه سادات حسینی
1
(گروه میکروبیولوژی.دانشکده علوم،دانشگاه آزاد اسلامی واحد کرمان،)
اشرف کریمی نیک
2
(دانشگاه آزاد اسلامی واحد کرمان ، عضو هیات علمی)
الهام نصیری
3
(گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی واحد کرمان، کرمان، ایران)
الکلمات المفتاحية: سودوموناس آئروژینوسا, عفونت خونی, RAGE, CXCL11,
ملخص المقالة :
سابقه و هدف: شناسایی باکتری های عامل عفونت خونی و تعامل آن با سیستم ایمنی،اهمیت بسیار زیادی دارد.RAGE نوعی گیرنده سلولی است که مولکول های با منشا درونی و خارجی را شناسایی می کند و CXC11 نیز درالقای پاسخ های ایمنی مناسب علیه میکروب ها شرکت می کند. هدف از این تحقیق،تعیین میزان سطح بیان این مولکول ها در بیماران با عفونت خونی و افراد سالم می باشد.مواد و روش ها: دراین مطالعه مورد-شاهدی، سطح بیان ژن RAGE و CXCL11 در 40 بیمار مبتلا به عفونت خونی و 40 فرد سالم به عنوان کنترل با استفاده از تکنیک Real time-PCRمورد بررسی قرار گرفت. تشخیص عفونت خونی با انجام کشت خون و بکارگیری آزمون های بیوشیمیایی باکتریایی انجام شد.یافته ها: سطح mRNA ژن RAGE در بیماران آلوده به سودوموناس آئروژینوسا در مقایسه با اشریشیا کلی ، استافیلوکوکوس اورئوس و اسینتوباکتر بامانی، به طور قابل توجهی افزایش داشت.اما سطح بیان CXCL11 در بین بیماران و گروه کنترل سالم افزایش معنی داری نداشت. همچنین در مقایسه باکتری های عامل عفونت ارتباط معنی داری مشاهده نگردید.نتیجه گیری: نتایج نشان داد که درعفونت خونی RAGE گیرنده مهمی در برابر سودوموناس آئروژینوسا می باشد. از این رو روش های کاهش میزان بیان مولکول هایRAGE ، می تواند نقش کاربردی در پاک سازی خون از این باکتری داشته باشد.
Emerg Med. 2019; 2 (1):19.
2. Cirz RT, Chin JK, Andes DR, de Crécy-Lagard , Craig WA, Romesberg E. Inhibition of
mutation and combating the evolution of antibiotic resistance. PLoS Biol. 2005; 3(6): e1 6.
3. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated
quinolone resistance. Lancet Infect Dis. 2006; 6(10):629–640.
4. Berit EE,Santini MP,Gielen J,Meembor S,Kronke M,Krut o.identification and characterization
of bacterial pathogens causing blood stream infections by DNA microarray .J Clin
Microbiol.2006 ;44( ):2389-239 .
5. Theresa AR, McKoy JM, Sepsis in Older Adults. Infect Dis Clin N Am. 201 ; 31: 731–742.
6. Bagheri , Askari A, Arababadi MK, Kennedy D. Can Toll-Like Receptor (TLR) 2 be
considered as a new target for immunotherapy against hepatitis B infection? Hum. Immunol.
2014; 5(6):549-54.
7. Karimi-Googheri M, Arababadi MK. TLR3 plays significant roles against hepatitis B virus.
Mol Biol Rep. 2014; 41(5):32 9-86.
8. Kang JH, Hwang SM, Chung I . S100A8, S100A9 and S100A12 activate airway epithelial
cells to produce MUC 5 AC via extracellular signal‐regulated kinase and nuclear factor‐ B
pathways. Immunol. 2015; 144(1): 9-90.
9. ranklin TC, Wohleb ES, Zhang , ogaça M, Hare B, Duman RS. Persistent increase in
microglial RAGE contributes to chronic stress–induced priming of depressive-like behavior.
Biol Psychiatry. 2018; 83(1):50-60.
10. Panezai J, Ghaffar A, Altamash M, Sundqvist K-G, Engström P-E, Larsson A. Correlation of
serum cytokines, chemokines, growth factors and enzymes with periodontal disease
parameters. PloS one. 201 ; 12(11): e0188945.
11. Erdel M, Laich A, Utermann G ,Werner E, Werner- elmayer G. The human gene encoding
SC B9B, a putative novel CXC chemokine, maps to human chromosome 4q21 like the
closely related genes for MIG (SC B9) and INP10 (SC B10). Cytogenet Genome Res.
1998; 81(3-4):2 1-2.
12. Ball JA , lisidou I, Blunt MD, Wood W, Ward SG. Hydrogen peroxide triggers a dual
signaling axis to selectively suppress activated human T lymphocyte migration. J. Immunol.
201 ; 198(9):36 9-89.
13. Kalil AC, Opal SM. Sepsis in the severely immunocompromised patient. Curr. Infect. Dis.
Rep. 2015; 1 (6):32.
14. Asadpour-Behzadi A, Kariminik A. RIG-1 and MDA5 are the important intracellular sensors
against bacteria in septicemia suffering patients. J. Appl. Biomed. 2018; 16(4):58-61.
15. orbes BA, Sahm D , Weissfeld AS. Diagnostic microbiology: Mosby St. Louis; 1998.
16. Afshari A. aghobi R. Karimi MH. Darbooie M. Azarpira N. 2014. Interleukin- 1 gene
expression and serum levels in acute rejected and non-rejected liver transplant patients. Iran J
Immunol .11:29-39.
17. Kariminik A, aghobi R, Dabiri S. CXCL9 expression and polyomavirus BK infectivity in
renal transplant patients with nephropathy. Cell Mol Biol. 2016; 62(1):104-8.
18. Kariminik A, aghobi R, Dabiri S. Association of BK irus Infection with CXCL11 gene
expression and protein levels in kidney transplant patients. Exp Clin Transplant. 2018; 16
(1):50-4.
19. Kariminik A, Dabiri S, aghobi R. Polyomavirus BK induces inflammation via up-regulation
of CXCL10 at translation levels in renal transplant patients with nephropathy. Inflammation.
2016; 39(4):1514-9.
20. Rezaeian A, aghobi R, Geramizadeh Increase in the level of MMP-2 gene expression in liver cirrhotic patients without chronic viral hepatitis B and C infections. J. Microbial
World .2019; 12(1):6-14.
21. Mohammadi Z, Momtaz H. Molecular typing of the Acinetobacter baumannii strains isolated
from blood infections using Multi Locus Sequence Typing (MLST). J. Microbial World.201 ;
10(2):104-113.
22. Denstaedt SJ, Singer BH, Standiford TJ. Sepsis and nosocomial infection: patient
characteristics, mechanisms, and modulation. ront Immunol. 2018; 23(9):2446.
23. Obritsch MD, ish DN, MacLaren R, Jung R. Nosocomial infections due to
multidrug‐resistant Pseudomonas aeruginosa: epidemiology and treatment options.
Pharmacotherapy. 2005; 25(10):1353-64.
24. Kariminik A, Baseri-Salehi M, Kheirkhah B. Pseudomonas aeruginosa quorum sensing
modulates immune responses: an updated review article. Immunol. Lett. 201 ; 190:1-6.
25. Schnurr M, Duewell P. Induction of immunogenic cell death by targeting RIG-I-like helicases
in pancreatic cancer. Oncoimmunol. 2014; 3(9): e95568 .
26. Selvaraj , Manne ND, Arvapalli R, Rice KM, Nandyala G, ankenhanel E, et al. Effect of
cerium oxide nanoparticles on sepsis induced mortality and N - B signaling in cultured
macrophages. Nanomedicine. 2015; 10(8):12 5-88.
27. Gao , ang Z, eng X , an TT, Jiang L, Guo R, et al. Interleukin-2 is elevated in
sepsis-induced myocardial dysfunction and mediates inflammation. Cytokine. 2016; 88:1-11.
28. Matsukura S, Kokubu , Kurokawa M, Kawaguchi M, Ieki K, Kuga H, et al. Role of RIG-I,
MDA-5, and PKR on the expression of inflammatory chemokines induced by synthetic
dsRNA in airway epithelial cells. Int. Arch. Allergy Immunol. 200 ; 143(1):80-3.
_||_
Emerg Med. 2019; 2 (1):19.
2. Cirz RT, Chin JK, Andes DR, de Crécy-Lagard , Craig WA, Romesberg E. Inhibition of
mutation and combating the evolution of antibiotic resistance. PLoS Biol. 2005; 3(6): e1 6.
3. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated
quinolone resistance. Lancet Infect Dis. 2006; 6(10):629–640.
4. Berit EE,Santini MP,Gielen J,Meembor S,Kronke M,Krut o.identification and characterization
of bacterial pathogens causing blood stream infections by DNA microarray .J Clin
Microbiol.2006 ;44( ):2389-239 .
5. Theresa AR, McKoy JM, Sepsis in Older Adults. Infect Dis Clin N Am. 201 ; 31: 731–742.
6. Bagheri , Askari A, Arababadi MK, Kennedy D. Can Toll-Like Receptor (TLR) 2 be
considered as a new target for immunotherapy against hepatitis B infection? Hum. Immunol.
2014; 5(6):549-54.
7. Karimi-Googheri M, Arababadi MK. TLR3 plays significant roles against hepatitis B virus.
Mol Biol Rep. 2014; 41(5):32 9-86.
8. Kang JH, Hwang SM, Chung I . S100A8, S100A9 and S100A12 activate airway epithelial
cells to produce MUC 5 AC via extracellular signal‐regulated kinase and nuclear factor‐ B
pathways. Immunol. 2015; 144(1): 9-90.
9. ranklin TC, Wohleb ES, Zhang , ogaça M, Hare B, Duman RS. Persistent increase in
microglial RAGE contributes to chronic stress–induced priming of depressive-like behavior.
Biol Psychiatry. 2018; 83(1):50-60.
10. Panezai J, Ghaffar A, Altamash M, Sundqvist K-G, Engström P-E, Larsson A. Correlation of
serum cytokines, chemokines, growth factors and enzymes with periodontal disease
parameters. PloS one. 201 ; 12(11): e0188945.
11. Erdel M, Laich A, Utermann G ,Werner E, Werner- elmayer G. The human gene encoding
SC B9B, a putative novel CXC chemokine, maps to human chromosome 4q21 like the
closely related genes for MIG (SC B9) and INP10 (SC B10). Cytogenet Genome Res.
1998; 81(3-4):2 1-2.
12. Ball JA , lisidou I, Blunt MD, Wood W, Ward SG. Hydrogen peroxide triggers a dual
signaling axis to selectively suppress activated human T lymphocyte migration. J. Immunol.
201 ; 198(9):36 9-89.
13. Kalil AC, Opal SM. Sepsis in the severely immunocompromised patient. Curr. Infect. Dis.
Rep. 2015; 1 (6):32.
14. Asadpour-Behzadi A, Kariminik A. RIG-1 and MDA5 are the important intracellular sensors
against bacteria in septicemia suffering patients. J. Appl. Biomed. 2018; 16(4):58-61.
15. orbes BA, Sahm D , Weissfeld AS. Diagnostic microbiology: Mosby St. Louis; 1998.
16. Afshari A. aghobi R. Karimi MH. Darbooie M. Azarpira N. 2014. Interleukin- 1 gene
expression and serum levels in acute rejected and non-rejected liver transplant patients. Iran J
Immunol .11:29-39.
17. Kariminik A, aghobi R, Dabiri S. CXCL9 expression and polyomavirus BK infectivity in
renal transplant patients with nephropathy. Cell Mol Biol. 2016; 62(1):104-8.
18. Kariminik A, aghobi R, Dabiri S. Association of BK irus Infection with CXCL11 gene
expression and protein levels in kidney transplant patients. Exp Clin Transplant. 2018; 16
(1):50-4.
19. Kariminik A, Dabiri S, aghobi R. Polyomavirus BK induces inflammation via up-regulation
of CXCL10 at translation levels in renal transplant patients with nephropathy. Inflammation.
2016; 39(4):1514-9.
20. Rezaeian A, aghobi R, Geramizadeh Increase in the level of MMP-2 gene expression in liver cirrhotic patients without chronic viral hepatitis B and C infections. J. Microbial
World .2019; 12(1):6-14.
21. Mohammadi Z, Momtaz H. Molecular typing of the Acinetobacter baumannii strains isolated
from blood infections using Multi Locus Sequence Typing (MLST). J. Microbial World.201 ;
10(2):104-113.
22. Denstaedt SJ, Singer BH, Standiford TJ. Sepsis and nosocomial infection: patient
characteristics, mechanisms, and modulation. ront Immunol. 2018; 23(9):2446.
23. Obritsch MD, ish DN, MacLaren R, Jung R. Nosocomial infections due to
multidrug‐resistant Pseudomonas aeruginosa: epidemiology and treatment options.
Pharmacotherapy. 2005; 25(10):1353-64.
24. Kariminik A, Baseri-Salehi M, Kheirkhah B. Pseudomonas aeruginosa quorum sensing
modulates immune responses: an updated review article. Immunol. Lett. 201 ; 190:1-6.
25. Schnurr M, Duewell P. Induction of immunogenic cell death by targeting RIG-I-like helicases
in pancreatic cancer. Oncoimmunol. 2014; 3(9): e95568 .
26. Selvaraj , Manne ND, Arvapalli R, Rice KM, Nandyala G, ankenhanel E, et al. Effect of
cerium oxide nanoparticles on sepsis induced mortality and N - B signaling in cultured
macrophages. Nanomedicine. 2015; 10(8):12 5-88.
27. Gao , ang Z, eng X , an TT, Jiang L, Guo R, et al. Interleukin-2 is elevated in
sepsis-induced myocardial dysfunction and mediates inflammation. Cytokine. 2016; 88:1-11.
28. Matsukura S, Kokubu , Kurokawa M, Kawaguchi M, Ieki K, Kuga H, et al. Role of RIG-I,
MDA-5, and PKR on the expression of inflammatory chemokines induced by synthetic
dsRNA in airway epithelial cells. Int. Arch. Allergy Immunol. 200 ; 143(1):80-3.
