اثر هیپولیپیدمیک عصاره هیدرومتانلی دانه شنبلیله (Trigonella foenum-graecum L) بر سطح لیپیدهای سرم و بیان PPAR-gamma در بافت چربی موش‌های صحرایی نر هیپرلیپیدمیک
الموضوعات :
مریم عیدی
1
(دانشگاه آزاد اسلامی - واحد ورامین - پیشوا - گروه زیست شناسی)
مهدیه محسنی
2
(گروه ژنتیک، دانشکده علوم زیستی، واحد ورامین - پیشوا، دانشگاه آزاد اسلامی، ورامین - پیشوا، ایران)
تاريخ الإرسال : 03 الإثنين , محرم, 1444
تاريخ التأكيد : 28 الخميس , جمادى الأولى, 1444
تاريخ الإصدار : 05 الأحد , محرم, 1445
الکلمات المفتاحية:
موش صحرایی,
دانه شنبلیله,
هیپرلیپیدمیا,
رسپتور فعال کننده تکثیر پراکسیزومی – گاما,
ملخص المقالة :
هیپرلیپیدمیا به عنوان یکی از مهم‌ترین عوامل خطرزای بیماری‌های قلبی-عروقی مطرح می‌باشد. هدف از مطالعه حاضر، بررسی اثر عصاره هیدرومتانلی دانه شنبلیله بر سطح لیپیدهای سرم و بیان PPAR-gamma در بافت چربی موش‌های صحرایی هیپرلیپیدمیک است. در این مطالعه تجربی، 36 سر موش صحرایی نر نژاد ویستار به صورت کاملاً تصادفی به شش گروه مساوی تقسیم شدند که شامل گروه کنترل سالم، گروه کنترل هیپرلیپیدمیک (دریافت کننده رژیم غذایی با 10 درصد چربی)، گروه هیپرلیپیدمیک دریافت کننده آتورواستاتین (غلظت 10 میلی‌گرم بر کیلوگرم وزن بدن) و گروه‌های تجربی هیپرلیپیدمیک دریافت کننده عصاره هیدرومتانلی دانه شنبلیله (غلظت‌های 100، 200 و 400 میلی‌گرم بر کیلوگرم وزن بدن) بودند. پس از 8 هفته تیمار، حیوانات بمدت 12 ساعت ناشتایی داده شده و سپس توزین و توسط اتر بیهوش گردیدند و نمونه خون از قلب و بافت چربی از ناحیه شکمی حیوانات جمع آوری شدند. میزان افزایش وزن، ضریب کبدی، سطح کلسترول، تری گلیسرید، LDL، HDL، AST وALT سرم توسط کیت و تغییر بیان PPAR-gamma به روش real-time PCR اندازه‌گیری شدند. نتایج نشان داد تیمار خوراکی آتورواستاتین و عصاره دانه شنبلیله موجب کاهش معنی دار وزن بدن، سطح کلسترول، تری گلیسرید، LDL، ، ALT و AST سرم و بیان PPAR-gamma در بافت چربی و افزایش معنی دار سطح سرمی HDL در گروه های تجربی هیپرلیپیدمیک در مقایسه با گروه کنترل هیپرلیپیدمیک می گردد. بنابراین، عصاره هیدرومتانلی دانه شنبلیله دارای خاصیت کاهنده چربی سرم است و با بهبود پروفایل لیپیدی سرم بیان رسپتور فعال کننده تکثیر پراکسیزوم ها را کاهش می دهد.
المصادر:
Altmann SW, Davis HR Jr, Zhu LJ, Yao X, Hoos LM, Tetzloff G, Iyer SP, Maguire M, Golovko A, Zeng M, Wang L, Murgolo N, Graziano MP. Niemann-pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science, 2004; 303(5661): 1201-1204.
Brouwers MC, van Greevenbroek MM, Stehouwer CD, de Graaf J, Stalenhoef AF. The genetics of familial combined hyperlipidaemia. Nat Rev Endocrinol, 2012; 8(6): 352-362.
Ezeh KJ, Ezeudemba O. Hyperlipidemia: A review of the novel methods for the management of lipids. Cureus, 2021; 13(7): e16412.
Tien N, Wu TY, Lin CL, Wu CJ, Hsu CY, Fang YJ, Lim YP. Impact of inflammatory bowel disease (IBD) and IBD medications on risk of hyperlipidemia and in vitro hepatic lipogenic-related gene expression: A population-based cohort study. Front Med, 2022; 9: 910623.
Rogue A, Lambert C, Jossé R, Antherieu S, Spire C, Claude N, Guillouzo A. Comparative gene expression profiles induced by PPARγ and PPARα/γ agonists in human hepatocytes. PLoS One, 2011; 6(4): e18816-10.
Acharya S, Srichamroen A, Basu S, Ooraikul B, Basu T. Improvement in the nutraceutical properties of fenugreek (Trigonella foenum-graecum). Songklanakarin. J Sci Technol, 2006; 28(1-9): 1-9.
Kumar D, Singhal A, Bansal S, Gupta SK. Extraction, islolation and evaluation Trigonella foenum-graecum as mucoadhesive agent for nasal gel drug delivery. J NPA, 2014; 27(1): 1-7.
Verma S, Bansal J, Kumar N, Malviya R, Sharma PK. Isolation and characterization studies of mucilage obtained from Trigonella foenum-graecum seed and Tamarindus indica polysaccharide as a pharmaceutical excipient. J Drug Delivery & Therapy, 2014; 4(3): 106-109.
Wani SA, Kumar P. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J Saudi Soc Agric Sci, 2018; 17(2): 97-106.
Yadav R, Kaushik R, Gupta D. The health benefits of Trigonella foenum-graecum: a review. Int J Eng Res Appl, 2011; 1(1): 32-35.
Reddy GD, Reddy AG, Rao GS, Kumar MV. Pharmacokinetic interaction of garlic and atorvastatin in dyslipidemic rats. Indian J Pharmacol, 2012; 44(2): 246-252.
Roughani M, Baluchnejadmojarad T, Roghani Dehlordi F. Hypolipidemic effect of aqueous leaf extract of Trigonella foenum-graecum in diabetic rats. Iranian J Endocrinol Metab, 2005; 7(2): 167-171.
Roohbakhsh E, Barari AR, Abbaszadeh H. The effect of interval training and consuming fenugreek seed extract on Fgf21 and Vegf gene expression in patients with coronary artery diseases. Ofogh-E-Danesh, 2021; 27(2): 130-146.
Yousefi E, Zavoshy R, Noroozi M, Jahani Hashemi H, Zareiy S, Alizadeh K, Ziari K. Effect of oral administration of fenugreek seeds powdered on lipid profile. Ebnesina, 2015; 17(1): 33-38.
Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K. Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rats. Lipids Health Dis, 2011; 10: 240.
Kassaee SM, Goodarzi MT, Kassaee SN. Ameliorative effect of Trigonella foenum-graecum on lipid profile, liver histology and LDL-receptor gene expression in high cholesterol-fed hamsters. Acta Endocrinol (Buc), 2021; 17(1): 7-13.
Mohammad-Sadeghipour M, Mahmoodi M, Noroozi Karimabad M, Mirzaei MR, Hajizadeh MR. Diosgenin and 4-hydroxyisoleucine from fenugreek are regulators of genes involved in lipid metabolism in the human colorectal cancer cell line SW480. Cell J, 2021; 22(4): 514-522.
Vijayakumar MV, Pandey V, Mishra GC, Bhat Hypolipidemic effect of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity, 2010; 18(4): 667-674.
Ibarra A, He K, Bai N, Bily A, Roller M, Coussaert A, Provost N, Ripoll C. Fenugreek extract rich in 4-hydroxyisoleucine and trigonelline activates PPARα and inhibits LDL oxidation: Key mechanisms in controlling the metabolic syndrome. Nat Prod Commun, 2008; 3(9): 1509-1513.
Ilavenil S, Arasu MV, Lee JC, Kim DH, Roh SG, Park HS, Choi GJ, Mayakrishnan V, Choi Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells. Phytomedicine, 2014; 21(5): 758-765.
Zayed EA, Ainshoka AA, El-Shazly KA, El-Mosallamy AEMK, Zayed AA, El-Latif HAA. Fenugreek oil and metformin improve insulin resistance via increase of GLUT4 and PPARγ in metabolic syndrome‐induced rats. Asian J Res Reports Endocrinol, 2021; 4(1): 29-30.
Roberts KT. The potential of fenugreek (Trigonella foenum-graecum) as a functional food and nutraceutical and its effects on glycemia and lipidemia. J Med Food, 2011; 14(12): 1485e1489.
Abedinzade M, Nasri S, Omidi MJ, Porramezan B, Khanaki K. The effect of fenugreek (Trigonella foenum-graecum) seed and 17-bestradiol on serum apelin, glucose, lipids and insulin in ovariectomized rats. Biotech Health Sci, 2015; 2(3): e30402.
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Altmann SW, Davis HR Jr, Zhu LJ, Yao X, Hoos LM, Tetzloff G, Iyer SP, Maguire M, Golovko A, Zeng M, Wang L, Murgolo N, Graziano MP. Niemann-pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science, 2004; 303(5661): 1201-1204.
Brouwers MC, van Greevenbroek MM, Stehouwer CD, de Graaf J, Stalenhoef AF. The genetics of familial combined hyperlipidaemia. Nat Rev Endocrinol, 2012; 8(6): 352-362.
Ezeh KJ, Ezeudemba O. Hyperlipidemia: A review of the novel methods for the management of lipids. Cureus, 2021; 13(7): e16412.
Tien N, Wu TY, Lin CL, Wu CJ, Hsu CY, Fang YJ, Lim YP. Impact of inflammatory bowel disease (IBD) and IBD medications on risk of hyperlipidemia and in vitro hepatic lipogenic-related gene expression: A population-based cohort study. Front Med, 2022; 9: 910623.
Rogue A, Lambert C, Jossé R, Antherieu S, Spire C, Claude N, Guillouzo A. Comparative gene expression profiles induced by PPARγ and PPARα/γ agonists in human hepatocytes. PLoS One, 2011; 6(4): e18816-10.
Acharya S, Srichamroen A, Basu S, Ooraikul B, Basu T. Improvement in the nutraceutical properties of fenugreek (Trigonella foenum-graecum). Songklanakarin. J Sci Technol, 2006; 28(1-9): 1-9.
Kumar D, Singhal A, Bansal S, Gupta SK. Extraction, islolation and evaluation Trigonella foenum-graecum as mucoadhesive agent for nasal gel drug delivery. J NPA, 2014; 27(1): 1-7.
Verma S, Bansal J, Kumar N, Malviya R, Sharma PK. Isolation and characterization studies of mucilage obtained from Trigonella foenum-graecum seed and Tamarindus indica polysaccharide as a pharmaceutical excipient. J Drug Delivery & Therapy, 2014; 4(3): 106-109.
Wani SA, Kumar P. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J Saudi Soc Agric Sci, 2018; 17(2): 97-106.
Yadav R, Kaushik R, Gupta D. The health benefits of Trigonella foenum-graecum: a review. Int J Eng Res Appl, 2011; 1(1): 32-35.
Reddy GD, Reddy AG, Rao GS, Kumar MV. Pharmacokinetic interaction of garlic and atorvastatin in dyslipidemic rats. Indian J Pharmacol, 2012; 44(2): 246-252.
Roughani M, Baluchnejadmojarad T, Roghani Dehlordi F. Hypolipidemic effect of aqueous leaf extract of Trigonella foenum-graecum in diabetic rats. Iranian J Endocrinol Metab, 2005; 7(2): 167-171.
Roohbakhsh E, Barari AR, Abbaszadeh H. The effect of interval training and consuming fenugreek seed extract on Fgf21 and Vegf gene expression in patients with coronary artery diseases. Ofogh-E-Danesh, 2021; 27(2): 130-146.
Yousefi E, Zavoshy R, Noroozi M, Jahani Hashemi H, Zareiy S, Alizadeh K, Ziari K. Effect of oral administration of fenugreek seeds powdered on lipid profile. Ebnesina, 2015; 17(1): 33-38.
Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K. Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rats. Lipids Health Dis, 2011; 10: 240.
Kassaee SM, Goodarzi MT, Kassaee SN. Ameliorative effect of Trigonella foenum-graecum on lipid profile, liver histology and LDL-receptor gene expression in high cholesterol-fed hamsters. Acta Endocrinol (Buc), 2021; 17(1): 7-13.
Mohammad-Sadeghipour M, Mahmoodi M, Noroozi Karimabad M, Mirzaei MR, Hajizadeh MR. Diosgenin and 4-hydroxyisoleucine from fenugreek are regulators of genes involved in lipid metabolism in the human colorectal cancer cell line SW480. Cell J, 2021; 22(4): 514-522.
Vijayakumar MV, Pandey V, Mishra GC, Bhat Hypolipidemic effect of fenugreek seeds is mediated through inhibition of fat accumulation and upregulation of LDL receptor. Obesity, 2010; 18(4): 667-674.
Ibarra A, He K, Bai N, Bily A, Roller M, Coussaert A, Provost N, Ripoll C. Fenugreek extract rich in 4-hydroxyisoleucine and trigonelline activates PPARα and inhibits LDL oxidation: Key mechanisms in controlling the metabolic syndrome. Nat Prod Commun, 2008; 3(9): 1509-1513.
Ilavenil S, Arasu MV, Lee JC, Kim DH, Roh SG, Park HS, Choi GJ, Mayakrishnan V, Choi Trigonelline attenuates the adipocyte differentiation and lipid accumulation in 3T3-L1 cells. Phytomedicine, 2014; 21(5): 758-765.
Zayed EA, Ainshoka AA, El-Shazly KA, El-Mosallamy AEMK, Zayed AA, El-Latif HAA. Fenugreek oil and metformin improve insulin resistance via increase of GLUT4 and PPARγ in metabolic syndrome‐induced rats. Asian J Res Reports Endocrinol, 2021; 4(1): 29-30.
Roberts KT. The potential of fenugreek (Trigonella foenum-graecum) as a functional food and nutraceutical and its effects on glycemia and lipidemia. J Med Food, 2011; 14(12): 1485e1489.
Abedinzade M, Nasri S, Omidi MJ, Porramezan B, Khanaki K. The effect of fenugreek (Trigonella foenum-graecum) seed and 17-bestradiol on serum apelin, glucose, lipids and insulin in ovariectomized rats. Biotech Health Sci, 2015; 2(3): e30402.
