The effect of biosynthesized silver nanoparticles on FAE1 and FAD2 gene expression in Camelina sativa
الموضوعات :Tayebehalsadat Mirmoeini 1 , Leila Pishkar 2 , Danial Kahrizi 3 , Giti Barzin 4 , Naser Karimi 5
1 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
2 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
3 - Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
4 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.
5 - Plant Physiology Laboratory, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
الکلمات المفتاحية: nanoparticles, Real Time PCR, Gene expression, Biosynthesize, Camelina,
ملخص المقالة :
Nanotechnology is a field of research related to physics, chemistry, engineering sciences with the application of new techniques and production of nanoscale materials and an emerging field in interdisciplinary research especially biotechnology. Camelina is an oilseed and re-emerging plant that requires a lot of research on its oil production process. This study was conducted to investigate the effect of biosynthesized silver nanoparticles on the expression level of FAE1 and FAD2 in Soheil cultivar of Camelina oilseed plant based on a completely randomized design with four replications in 2018-2019. The aqueous extract of Camelina leaf and silver nitrate salt was used to prepare the nanoparticles. Experimental treatments included 0.5, 1, 2 and 3 mg / L of silver nanoparticles. After the preparation of foliar samples for all treatments, RNA extraction, cDNA synthesis and temperature gradient determination, the Real Time PCR reaction was used to study gene expression patterns. Data were then analyzed using GenEX and SAS software. The results showed that the effect of silver nanoparticles on FAE1 and FAD2 gene expression was significant (p<0.05) and the effect was increased with increasing silver nanoparticle concentration. The highest enhancement was observed at 3 mg / L silver nanoparticles.
Ahmed, S., M. Ahmad, B. L. Swami and S. Ikram. 2016. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Journal of advanced research, 7, (1) 17-28.
Ansari, F. and D. Kahrizi. 2018. Hydrothermal synthesis of highly fluorescent and non-toxic carbon dots using Stevia rebaudiana Bertoni. Cellular and Molecular Biology, 64, (12) 32-36.
Babaei, Z., M. Solouki and B. Fazeli-Nasab. 2019. Investigating The Effect of Biological and non-Biological Elicitor on Expression of Hyp-1 Gene in Hypericum perforatum. Modern Genetics,13, (4) 543-549.
Biswas, P. and C.-Y. Wu. 2005. Nanoparticles and the environment. Journal of the air & waste management association, 55, (6) 708-746.
Boxall, A., Q. Chaudhry, C. Sinclair, A. Jones, R. Aitken, B. Jefferson and C. Watts. 2007. Current and future predicted environmental exposure to engineered nanoparticles. Report by the Central Science Laboratory (CSL) York for the Department of the Environment and Rural Affairs (DEFRA), UK. wwTv de-fra gov uk/science/Project_Da-ta/DocumentLibrary/CB01098/CB01098_627 () _FRP pdf,
Cahoon, E. B., K. G. Ripp, S. E. Hall and A. J. Kinney. 2001. Formation of conjugated Δ8, Δ10-double bonds by Δ12-oleic-acid desaturase-related enzymes: biosynthetic origin of calendic acid. Journal of Biological Chemistry, 276, (4) 2637-2643.
Campbell, M. 2018. Camelina–an alternative oil crop. In Biokerosene:259-275: Springer. Number of 259-275 pp.
Campbell, M., A. Rossi and W. Erskine. 2013. Camelina (Camelina sativa (L.) Crantz): agronomic potential in Mediterranean environments and diversity for biofuel and food uses. Crop and Pasture Science, 64, (4) 388-398.
Childs, P. G., C. A. Boyle, G. D. Pemberton, H. Nikukar, A. S. Curtis, F. L. Henriquez, M. J. Dalby and S. Reid. 2016. Use of nanoscale mechanical stimulation for control and manipulation of cell behaviour. Acta Biomaterialia, 34, 159-168.
Fouda, M. M., N. R. Abdelsalam, M. E. El-Naggar, A. F. Zaitoun, B. M. Salim, M. Bin-Jumah, A. A. Allam, S. A. Abo-Marzoka and E. E. Kandil. 2020. Impact of high throughput green synthesized silver nanoparticles on agronomic traits of onion. International journal of biological macromolecules, 149, 1304-1317.
Ghamarnia, H., D. Kahrizi and H. Rostami-Ahmadvandi. 2020. Camelina; a low input and compatible plant. Razi University press Press (in Persian).
Ghamkhar, K., J. Croser, N. Aryamanesh, M. Campbell, N. Kon’kova and C. Francis. 2010. Camelina (Camelina sativa (L.) Crantz) as an alternative oilseed: molecular and ecogeographic analyses. Genome, 53, (7) 558-567.
Gishini, M. F. S., A. Zebarjadi, M. Abdoli-Nasab, M. J. Javaran, D. Kahrizi and D. Hildebrand. 2020. Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes. Functional & integrative genomics, 20, (3) 433-458.
Gorth, D. J., D. M. Rand and T. J. Webster. 2011. Silver nanoparticle toxicity in Drosophila: size does matter. International journal of nanomedicine, 6, 343.
Husen, A. and K. S. Siddiqi. 2014. Phytosynthesis of nanoparticles: concept, controversy and application. Nanoscale research letters, 9, (1) 1-24.
Hutcheon, C., R. F. Ditt, M. Beilstein, L. Comai, J. Schroeder, E. Goldstein, C. K. Shewmaker, T. Nguyen, J. De Rocher and J. Kiser. 2010. Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes. BMC plant biology, 10, (1) 1-15.
Jegadeeswaran, P., R. Shivaraj and R. Venckatesh. 2012. Green synthesis of silver nanoparticles from extract of Padina tetrastromatica leaf. Digest Journal of Nanomaterials and Biostructures, 7, (3) 991-998.
Khodayari, M., M. Omidi, A. Shahnejat Booshehri, D. Yazdani and M. Naghavi. 2015. Gene expression involved in sanguinarine biosynthesis is affected by nano elicitors in Papaver somniferum L. Journal of Medicinal Plants, 14, (54)
Khodayari M, M. Omidi, A. Shah-Najat-Bushehri and M. Shit .2015. 'Biological Elistor and Nano-Elistor Effect on Gene Expression of Some Populonic Alkaloids'. Iranian Horticultural Science, 45: 287-295.
Li, L., X. Wang, J. Gai and D. Yu. 2008. Isolation and characterization of a seed-specific isoform of microsomal omega-6 fatty acid desaturase gene (FAD2-1B) from soybean: Full Length Research Article. DNA Sequence, 19, (1) 28-36.
Livak, K. J. and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25, (4) 402-408.
Monica, R. C. and R. Cremonini. 2009. Nanoparticles and higher plants. Caryologia, 62, (2) 161-165.
Nelson, P., E. Hood and R. Powell. 2011. The bioeconomy: a new era of products derived from renewable plant-based feedstocks. Plant Biomass Convers, 3-20.
Nowack, B., H. F. Krug and M. Height. 2011. 120 years of nanosilver history: implications for policy makers. ACS Publications.
Obour, A., H. Sintim, E. Obeng and D. Jeliazkov. 2015. Oilseed camelina (Camelina sativa L Crantz): Production systems, prospects and challenges in the USA Great Plains. Adv Plants Agric Res, 2, (2) 00043.
Park, S.-U., M. Yu and P. J. Facchini. 2003. Modulation of berberine bridge enzyme levels in transgenic root cultures of California poppy alters the accumulation of benzophenanthridine alkaloids. Plant molecular biology, 51, (2) 153-164.
Peng, Q., Y. Hu, R. Wei, Y. Zhang, C. Guan, Y. Ruan and C. Liu. 2010. Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds. Plant cell reports, 29, (4) 317-325.
Rico, C. M., S. Majumdar, M. Duarte-Gardea, J. R. Peralta-Videa and J. L. Gardea-Torresdey. 2011. Interaction of nanoparticles with edible plants and their possible implications in the food chain. Journal of agricultural and food chemistry, 59, (8) 3485-3498.
Salata, O. V. 2004. Applications of nanoparticles in biology and medicine. Journal of nanobiotechnology, 2, (1) 1-6.
Schearer, W. 1984. Components of oil of tansy (Tanacetum vulgare) that repel Colorado potato beetles (Leptinotarsa decemlineata). Journal of Natural Products, 47, (6) 964-969.
Sharma, V. K., R. A. Yngard and Y. Lin. 2009. Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in colloid and interface science, 145, (1-2) 83-96.
Shi, J., C. Lang, F. Wang, X. Wu, R. Liu, T. Zheng, D. Zhang, J. Chen and G. Wu. 2017. Depressed expression of FAE1 and FAD2 genes modifies fatty acid profiles and storage compounds accumulation in Brassica napus seeds. Plant Science, 263, 177-182.
Tran, Q. H. and A.-T. Le. 2013. Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Advances in Natural Sciences: Nanoscience and Nanotechnology, 4, (3) 033001.
Vasconsuelo, A. and R. Boland. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant science, 172, (5) 861-875.
Waraich, E. A., Z. Ahmed, R. Ahmad, M. Y. Ashraf, M. S. Naeem and Z. Rengel. 2013. 'Camelina sativa', a climate proof crop, has high nutritive value and multiple-uses: A review. Australian Journal of Crop Science, 7, (10) 1551-1559.
Yang, H. G., C. H. Sun, S. Z. Qiao, J. Zou, G. Liu, S. C. Smith, H. M. Cheng and G. Q. Lu. 2008. Anatase TiO 2 single crystals with a large percentage of reactive facets. Nature, 453, (7195) 638-641.
Yousefi, K., A. Riahi and A. Baghizadeh. 2015. Investigation of the effects of Ag and Cu elicitors on flavone synthase 1 gene expression and some biochemical parameters on Cuminum cyminum L. endemic from Iran. Journal of Plant Research (Iranian Journal of Biology), 28, (1) 210-223.