AMELX and AMELY Structure and Application for Sex Determination of Iranian Maral deer (Cervus elaphus maral)
الموضوعات :ط. فرهوش 1 , ر. واعظ ترشیزی 2 , ع.ا. مسعودی 3 , ح.ر. رضایی 4 , م. تولایی 5
1 - Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 - Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
3 - Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
4 - Department of Environmental Science, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Science and Natural Recourses, Gorgan, Iran
5 - Human Genetic Research Center, Baqiyatallah University of Medical Science, Tehran, Iran
الکلمات المفتاحية: amelogenin, <i>Cervus elaphus maral</i>, sex determination, wilderness,
ملخص المقالة :
In order to have a good perspective of wild animals, it is necessary to determine their population and genetic structure. It provides an opportunity to decide on better conservationmanagements. Inthe wilderness, due to the escapable nature and sometimes not havingthe distinguishable bisexual appearance, sex identification could be difficult by observing animals. The X- and Y- chromosome linked amelogenin (AMELX and AMELY) due to its independent and different evolution on both chromosomes could play an important role in sex determining of wild animals. To determine the sex ratio and alsothe genetic structureof AMELX and AMELY in Maral deer (Cervus elaphus maral), 37 sampleswere collected from populations were located in north parts of Iran. Results showed that in female deer, the amelogenin gene had one banding patterns (231bp, for X chromosome) and the male deer had two banding pattern (231 bp and 180 bp for X and Y chromosomes, respectively). The AMELY of Maral had in/del mutation (54 bp). The genetic distance (D) of AMELX from Maral deer and Red deer was 0.12 ± 0.02, it was calculated zero for AMELY. The phylogenetic analysis of AMELX and AMELY of different deer species, showed no distance for AMELY and the D was 0.048 ± 0.009 for AMELX. It is recommended that sex determination of wild animals, especially mammalian populations using amelogenin gene would be a useful and simple method which could provide further information for genetic conservation strategies.
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