Survivability of Kedu Rooster Sperm at Different Storage Times and Diluent Supplemented with Vitamin E and Fertilizing Ability on Kampung Chicken
الموضوعات :
S. Lestari
1
(Department of Animal Science, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
D.M. Saleh
2
(Animal Physiology and Reproduction Laboratory, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
I. Ismoyowati
3
(Poultry Production Laboratory, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
M.Y. Sumaryadi
4
(Animal Physiology and Reproduction Laboratory, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
E. Tugiyanti
5
(Poultry Production Laboratory, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
N. Hidayat
6
(Poultry Production Laboratory, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia)
الکلمات المفتاحية: viability, Fertility, motility, fertile period, plasma membrane integrity,
ملخص المقالة :
In the present study, the effect and interaction of the addition of vitamin E in diluents during storage were investigated. Semen samples were collected from ten Kedu roosters. Analysis of spermatozoa quality used analysis of variance based on a randomized block factorial design. The first factor was lactate ringer (D1) and skim milk + 50 mM glucose (D2), the second factor was a dose of vitamin E (V0-3= 0, 1, 2, 3%) and the third factor was storage time at 4 ˚C (T0-3= 0, 2, 4, 6 hours). The analysis of variance showed that there was an interaction between type of diluents and vitamin E (P<0.01) on sperm viability and abnormalities, with the best combination at D1V2. The type of diluent had a significant effect (P<0.05) on sperm motility. The vitamin E dose had a significant effect on membrane integrity. Storage time had a high effect on sperm motility. There were eight combinations among the type of diluent, vitamin E dose, and storage time, which had a significant effect (P<0.01) on fertility and the fertile periods of kampung hen eggs. The combination of D2V2T0 showed the best fertility and the D2V0T0 showed the best fertile periods. In conclusion, the addition of vitamin E 2% in lactate ringer diluents was better for maintaining the quality of spermatozoa. Artificial insemination tests in kampung hens showed fresh semen in a combination diluent of skimmed milk + 50 mM glucose and vitamin E 2% resulting in the highest fertility and fertile period.
Abdillah L., Bintara S., Maharani D. and Budisatria I.G.S. (2021). Evaluasi penggunaan etanol dan surfaktan tween 80 dalam melarutkan vitamin e pada bahan pengencer sperma andromed. Bul. Pet. Trop. 2, 125-129.
Amaefule B.C., Uzochukwu I.E. and Ugwu S.O.C. (2020). Effect of dietary supplementation of vitamin c and e on the semen quality of local turkeys. Agro-Sci. 19, 25-30.
Ansgarius F.A., Uly K., Kihe J.N., Henderiana L.L., Wilmientje M. and Nalley N. (2022). Quality of Bali cow semen with the addition of antioxidants and minerals zn (zinc) in egg yolk citrate diluent. J. Nukleus Peternakan. 9, 176-186.
Apell R.A. and Evans P.R. (1997). The effect of temperature on sperm motility and viability. Fertil. Steril. 12, 1329-1332.
Arifiantini R.I. (2012). Semen Collection and Evaluation Techniques in Animals. IPB Press, Bogor, Indonesia.
Ax R.L., Dally M., Didion B.A., Lenz R.W., Love C.C., Varner D.D., Hafez B. and Bellin M.E. (2008). Semen evaluation. Pp.146-147 in Reproduction in Farm Animal. B. Hafez and E.S.E. Hafez, Eds., Lippincott Williams & Wilkins, Philadelphia, USA.
Azzam H.A., Raharjo D., Hernawati T., Madyawati S.P. and Utomo B. (2022). Combination of 5% dextrose ringer’s solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature. Ovozoa. 11, 109-113.
Bakst M.R. and Dyamond J.S. (2013). Artificial insemination in poultry. Artificial insemination in poultry. Pp. 176-195 in Success in Artificial Insemination: Quality of Semen and Diagnostics Employed. A. Lemma, Ed., Intech Open Access Publisher, Zagreb, Croatia.
Bansal A.K. and Bilaspuri G.S. (2008). Effect of manganese on bovine sperm motility, viability, and lipid peroxidation in vitro. J. Anim. Reprod. 5, 90-96.
Barth A.D. and Oko R.J. (1989). Abnormal morphology of bovine spermatozoa. Lowa State University Press, Ames, Lowa.
Bebas W. and Laksmi D.N.D.I. (2015). Green junggle fowl sperm viability in eggyolk phosfat diluents containing lactose storaged at 5 oC. J. Vet. 16, 62-67.
Brillard J.P. (2003). Practical aspects of fertility in poultry. World's Poult. Sci. J. 59, 441-446.
Chakraborty S. and Saha S. (2022). Understanding sperm motility mechanims and the implication of sperm surface molecules in promoting motility. Middle East Fertil. Soc. J. 27, 1-12.
Chauychu-noo N., Thananurak P., Boonkum W., Vongpralub T. and Chankitisatul V. (2021). Effect of organic selenium dietary suplementation on quality and fertility of cryopreserved chicken sperm. Cryobiology. 98, 57-62.
Clarke R.N., Sexton T.J. and Ottinger M.A. (1982). Effect of holding temperature and storage time on respiratory rate, motility, and fertility of chicken and turkey semen. Poult. Sci. 61, 1912-1917.
Danang D.R., Isnaini N. and Trisunuwati P. (2012). The effect of long storage of semen on the quality of native chicken spermatozoa in ringer diluent at 4°C. J. Trop. Anim. Prod. 13, 47-57.
Devi G.S., Prasad M.H., Saraswathi I., Raghu D., Rao D.N. and Reddy P.P. (2000). Free radical antioxidant enzymes and lipid peroxidation in different types of leukemias. Clin. Chim. Acta. 293, 53-62.
Dorota S. and Kurpirsz M. (2004). Reactive oxygen species and sperm cell. J. Reprod. Biol. Endocrin. 2, 1-7.
Dumpala P.R., Parker H.M. and McDaniel C.D. (2006). The effect of semen storage temperature and diluent type on the sperm quality index of broiler breeder semen. Int. J. Poult. Sci. 5, 838-845.
Farid M., Arif M., Prihantoko K.D., Kusumawati A., Wijayanti A.D. and Setyawan E.M.N. (2021). Supplement effects of vitamin C, vitamin E and the combinations in semen extenders of kub chicken quality. Adv. Anim. Vet. Sci. 9, 1034-1039.
Fitriyah N., Humaidah H. and Suryanto D. (2019). The effect of long storage of semen in ringers lactat diluent stored at 4ºC on the quality of spermatozoa of magon chickens. J. Ilm. Petern. 1, 28-35.
Garner D.L. and Hafez E.S.E. (2008). Spermatozoa and seminal plasma reproduction in farm animal. Pp. 92-95 in Reproduction in Farm Animal. B. Hafez and E.S.E. Hafez, Eds., Lippincott Williams & Wilkins, Philadelphia, USA.
Gibb Z. and Aitken R.J. (2016). The impact of sperm metabolism during in vitro storage the stallion as model. Bio. Res. Int. 2, 1-8.
Gordon M.H. (1990). The mechanism of antioxidant action in vitro. Pp 1-18 in Food Antioxidants. B.J.F. Hudson, Ed., Springer, Dordrecht, United Kingdom.
Haq I., Mazumder P. and Kalamdhad A.S. (2020). Recent advances in removal of lignin from paper industry wastewater and its industrial applications-a review. Bio. Technol. 312, 123-636.
Hariyatmi H. (2004). Kemampuan vitamin e sebagai antioksidan terhadap radikal bebas pada usia lanjut. J. MIPA UMS. 14, 52-60.
Hidayat N., Hidayah C.N. and Nugroho A.P. (2020). Reduction spermatozoa motility and viability on various local chickens during storage at 5℃. J. Anim. Prod. 22, 158-162.
Hidayat N., Ismoyowati I., Hidayah C.N. and Nugroho A.P. (2019). Fertility and fertile period of duck eggs after artificial insemination with muscovy duck semen supplemented with vitamin C and E. J. Ked. Hewan. 13, 88-92.
Hoesni F. (2016). The effect of using skimmed milk with egg yolk tris diluent on the survival of bovine spermatozoa. J. Ilm. Univ. Batanghari Jambi. 16, 46-56.
Ismoyowati I., Sukardi S. and Santoso A. (2012). Genetic diversity of kedu chicken based on phenotypyic characteritics and microsatellite loci. Int. J. Poult. Sci. 11, 605-610.
Junaedi J., Arifiantini R.I. and Sumantri C. (2017). The quality of kampung broiler (kb) chicken frozen semen with DMA concentrations on yolk lactate ringer diluent. Chalaza J. Anim. Husband. 2, 19-24.
Karja N.W.K., Nofa Y. and Arifiantini R.I. (2017). Status akrosom dan kualitas post-thawed spermatozoa pada beberapa rumpun sapi dari dua balai inseminasi buatan. Acta Vet. Indonesian. 5, 81-88.
Khaeruddin K., Nurlinda A., Ardi N., Fattah A.H. and Armayanti A.K. (2020). Penentuan konsentrasi susu skim terbaik dalam pengencer semen ayam kampung berbahan dasar ringer laktat. J. Vet. 21, 300-308.
Khan I.M., Xu D., Cao Z., Liu H., Khan A., Rahman S.U., Ahmed J.Z., Raheem M.A. and Zhang Y. (2021). Addition of L-Cysteine and vitamin E to semen diluent enhances freeze-thawed spermatozoa characteristics in crossbred cattle bulls under subtropical environment. Pakistan J. Zool. 53, 1309-1319.
Kusumawati E.D., Krisnaningsih A.T.N. and Walangara A.U.K. (2020). Quality of spermatozoa of free-range chickens and Arabic chickens with different shelf life at room temperature. J. Sains Peternakan. 8, 41-56.
Long J.A. and Kramer M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid stored turkey semen. J. Poult. Sci. 82, 1802-1807.
Loziene K., Venskutonis P.R.., Sipailien A. and Labokas J. (2007). Radical scavenging and antibacterial properties of the extracts from different Thymus pulegioides chemotypes. Food Chem. 103, 546-559.
Malik A., Haron A.W., Yusoff R., Nesa M., Bukar M. and Kasim A. (2013). Evaluation of the ejaculate quality of the red jungle fowl, domestic chicken, and bantam chicken in Malaysia. Turky J. Vet. Anim. 37, 564-568.
Mariani Y. and Kartika N.M.A. (2018). The effect of the type of diluent and the concentration of scavenger chickens on the fertile period of arab chicken eggs. Gara. 12, 81-85.
Masrifah M., Rahman N. and Abram P.H. (2017). Uji aktivitas antioksidan ekstrak daun dan kulit labu air (Lagenaria siceraria (Molina) Standl.). J. Akad. Kim. 6, 98-106.
Mayesta D.D.M., Trilaksana I.N.B. and Bebas W. (2014). Motility and viability of chicken spermatozoa in glucose diluent yolk phosphate at 3-5 oC storage. Indonesian Med. Vet. 3, 43-52.
Modupe O., Livinus A.C. and Ifeany N.B. (2013). Semen quality characteristic and effect of mating ratio on reproductive performance of hubbarrd broiler breeders. J. Agri. Sci. 5, 154-159.
Mohan J., Sharma K., Kolluri G. and Dhama K. (2018). History of artificial insemination in poultry, it’s components and significance. World’s Poult. Sci. J. 74, 1-14.
Moradpour F. (2019). A Review on animals semen characteristics: Fertility, reproduction and development. Asian J. Adv. Agric. Res. 10, 1-9.
Mussa N.J., Boonkum W. and Chankitisakul V. (2023). Semen quality traits of two Thai native chickens producing a high and a low of semen volumes. Vet. Sci. 10, 73-79.
Nahak P.L., Dethan A.A. and Kia K.W. (2022). Quality of landrace pig semen in citrate-yolk semen diluent supplemented with glucose of different concentrations. J. Anim. Sci. 7, 12-15.
Nugroho A.P. and Saleh D.M. (2016). Motility and abnormalities of native chicken spermatozoa with lactate-egg white ringer diluent and long store at 5 ℃ for 48 hours. Acta Vet. Indonesia. 4, 35-41.
Pandia Y.M., Bebas W. and Pemayun T.G.O. (2021). Motilitas dan daya hidup spermatozoa ayam cemani dalam pengencer ringer laktat kuning telur pada penyimpanan suhu 4 °C. IndonesianMed. Vet. 10, 105-115.
Partyka A., Lukaszewicz E. and Nizannki W. (2012). Effect of cryopreservation on sperm parameters lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology. 77, 1497-1504.
Peters S.O., Shoyebo O.D., Ilori B.M., Ozoje M.O., Ikeobi C.O.N. and Adebambo O.A. (2008). Semen quality traits of seven strain of chickens raised in humid tropics. Int. J. Poult. Sci. 7, 949-953.
Putra I.M., Bebas W. and Budiasa M.K. (2019). The effect of adding various concentrations of vitamin E to yolk phosphate diluents on the motility and viability of quail spermatozoa. Bul. Vet. Udayana. 11, 58-64.
Putranto H.D., Nurmeliasari N. and Harferry K.T. (2020). Study of the quality of bergo chickens. Bull. Trop. Anim. 1, 10-15.
Revell S.G. and Mrode R.A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36, 77-86.
Rochmi S.E. and Sofyan M.S. (2019). A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C. Vet. World. 12, 1116-1120.
Saleh D.M., Sumaryadi M.Y., Nugroho A.P. and Hidayah C.N. (2022). Motility and fertility of native chicken spermatozoa in skim milk-based semen thinner. Pp. 54-61 in Proc. IXth Livest. Technol. Agribusin. Semin. Jenderal Soedirman Univ., Indonesia.
Shanmugam M. and Mahapatra R.K. (2023). Effect of tempol in sasaki diluent on the quality and fertility of cryopreserved chicken semen. Iranian J. Appl. Anim. Sci. 13, 187-190.
Susilawati S. and Hernawati T. (1992). The use of diluent of fruit solution for storing sheep semen. Med. Kedokteran Hewan. 3, 34-40.
Tabatabaei S., Batavani R. and Ayen E. (2011). Effect of vitamin E addition to chicken semen on sperm quality during in vitro storage of semen. Vet. Res. Forum. 2, 103-111.
Telnoni S.P., Arifiantini R.I. and Darwati S. (2021). Sperm fertility of SK Kedu chicken in lactated ringer’s-egg yolk extender with 10% of DMSO. Int. J. App. Biol. 5, 116-163.
Toelihere M.R. (1993). Artificial Insemination in Cattle. Angkasa Publisher, Bandung, Indonesian.
Triardi T., Ervandi M., Fahrullah F., Repi T. and Indrianti M.A. (2022). Kualitas semen ayam kub menggunakan pengencer ringer dextrose dan ringer laktat pada suhu 5℃. J. Pet. Sriwijaya. 11, 42-51.
Vahedi V., Evrigh N.H., Behroozlak M. and Dirandeh E. (2018). Antioxidant effects of thyme (Thymus vulgaris) extract on ram sperm quality during cryopreservation. Iranian J. Appl. Anim. Sci. 8, 263-269.
Vasicek J., Kuzelova L., Kulikova B. and Chrenek P. (2015). Effect of diluent and storage time on sperm characteristics of rooster insemination doses. Avian Biol. Res. 8, 41-46.
Yaman M.A., Reza M.A., Abdullah A.N., Usman Y. and Koesmara H. (2021). Sperm quality of hybrid chicken affected by propolis, honey, or royal jelly as organic diluent material and storage periods during sperm preservation. Pp. 206-272 in Proc. Int. Conf. Improv. Trop. Anim. Prod. Food Secur. Amsterdam, Netherlands.
Yendraliza Y., Anwar P. and Rodiallah M. (2015). Bioteknologi Reproduksi. Aswaja Presindo,Yogyakarta, Indonesian.
