The Effect of in ovo Injection of Royal Jelly on the Hatchability, Quality of One Day Broiler Chickens, the Performance and some Immune Related Traits in Hatched Chickens
الموضوعات :م. گوهری 1 , ح.ر. خدائی 2 , م. طغیانی 3
1 - Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Animal Science, Golpayegan Branch, Islamic Azad University, Isfahan, Iran
3 - Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
الکلمات المفتاحية: royal jelly, broiler chickens, immune system, <i>in ovo</i> injection,
ملخص المقالة :
In order to investigate the effect of in ovo injection of different levels of royal jelly on the hatchability rate and the quality of one-day-old broiler chickens (Ross 308) and some traits of the immune system, we have conducted an experiment using 640 eggs in a completely randomized design with four levels of royal jelly injection including 1) 1 mL of physiological serum (control), 2) 0.5 mg of royal jelly, 3) 0.75 mg of royal jelly, 4) 1 mg of jelly. For each treatment, four replicates were considered and 40 eggs were used in each replicate. On the 14th day of incubation, a test solution was injected into an allantoic fluid using an insulin syringe with a 2 cm needle length. After 510 hours of incubation, all of the hatched chicks were assessed with good, moderate and poor scores. The parameters studied during the breeding of the hatched eggs included weight gain, feed intake, feed conversion ratio, as well as the immune system traits. The in ovo injection of royal jelly did not include any difference in the hatching rate, but it significantly increased the quality of the one-day-old broiler chickens (P<0.05). In addition, the weight gain, feed intake and feed conversion ratio developed significantly as compared to the control group. The antibody titers was not affected by the experimental treatments, but it increased as a result of royal jelly injection. Regarding the performance of the birds throughout the breeding season, it can be inferred that the best performance and response of the immune system in hatched chicks was achieved when 0.5 mL of royal jelly was injected.
Bhanja S.K., Mandal A.B., Agarwal S.K., Majumdar S. and Bhattacharyya A. (2007). Effect of in ovo injection of vitamins on the chick weight and post-hatch growth performancein broiler chickens. Pp. 143-146 in Proc.16th European Symp. Poult. Nutr., Strasbourg, France.
Brooke D. and Humphrey A. (2010). The role of protein and amino acids in immunity. Crit. Rev. Poult. Biol. 16, 107-111.
Calder P.C. (2001). Polyunsaturated fatty acids, inflammation and immunity. Lipids. 3(9), 1007-1024.
Cook M.E. (1991). Nutrition and the immune response of the domestic fowl. Crit. Rev. Poult. Biol. 3, 167-189.
Ebrahimi M.R., Jafari Ahangari Y., Zamiri M.J., Akhlaghi A. and Atashi H. (2012). Doespre-incubational in ovo injection of buffers or antioxidants improve the quality and hatchability of long-term stored eggs? Poult. Sci. 91(11), 2970-2976.
Gore A.B. and Qureshi M.A. (1997). Enhancement of humoral andcellular immunity by vitamin E after embryonic exposure. Poult. Sci. 76, 984-991.
Graham M. (1992). The Hive and the Honey Bee. Dadant and Sons Inc., Hamilton, USA.
Jafari Ahangari Y., Hashemi S.R., Akhlaghi A., Atashi H., Esmaili Z., Ghorbani M., Mehmandoyi A., Mastani R., Azadegan A. and Davoodi H. (2012). Effect of in ovo injection of royal jelly on post-hatch growth performance and immune response in broiler chickens challenged with Newcastle disease virus. Iranian J. Appl. Anim. Sci. 3(1), 201-206.
Keralapurath M.M., Corzo A., Pulikanti R., Zhai W. and Peebles E.D. (2010). Effects of in ovo injection of L-carnitine on hatchability and subsequent broiler performance and slaughter yield. Poult. Sci. 89, 497-1501.
Kidd M.T., McDaniel C.D., Peebles E.D., Barber S.J., Corzo A., Branton S.L. and Woodworth J.C. (2005). Breeder hen dietary L-carnitine affects progeny carcase traits. Br. Poult. Sci. 46, 91-103.
Kocamis H., Yeni Y.N., Kirkpatrick-keller D.C. and Killefer J. (1999). Postnatal growth of broilers in response to in ovo administration of chicken growth hormone. Poult. Sci. 78, 1219-1226.
Kreukniet M.B., Nieuwland M.G.B. and Vander-Zijpp A.J. (1994). Phagocytic activity of two lines of chicks divergently selected for antibody production. Vet. Immunol. Immunophathol. 44, 377-387.
Marsteller F.A., Gross W.B. and Siegel P.B. (1980). Antibody production and Escherichia coli resistance in socially stable flock of dwarf and non-dwarfchicks. Poult. Sci. 59, 1974-1948.
Mousavi N., Shivazad M., Chamani M., Lotfollahian H. and Sadeghi A.A. (2011). The effects of injection of amino acid, carbohydrate and butyric acid in incubation eggs on the intestinal morphology and performance of broiler chickens. Iranian J. Anim. Sci. 42(2), 153-160.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Ohta Y., Tsushima N., Koide K., Kidd M.T. and Ishibashi T. (1999). Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks. Poult. Sci. 78, 1493-1498.
Pourreza J. (1991). Scientific and Practical Principles of Poultry Production. Arkan Danesh, Isfahan, Iran.
SAS Institute. (2005). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Singh U., Devaraj S. and Jialal I. (2005). Vitamin E, oxidative stress, and inflammation. Annu. Rev. Nutr. 25, 151-174.
Tona K., Bamelis F., Ketelaere B., Bruggeman V., Moraes V.M.B., Buyse J., Onagbesan O. and Decuypere E. (2003). Effects of egg storage time on spread of hatch, chick quality and chick juvenilegrowth. Poult. Sci. 82, 736-741.
Zhai W., Neuman S.L., Latour M.A. and Hester P.Y. (2008). The effect of male and female supplementation of L-carnitine on reproductive traits of white leghorns. Poult. Sci. 87, 1171-1181.
