Calpastatin Gene Polymorphism in Raini and Tali Goat in the Kerman Province
الموضوعات :
1 - Department of Animal Science, Technical and Vocational University, Kerman, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
الکلمات المفتاحية: Tali goat, genotype, calpastatin gene, PCR RFLP, Raeini goat,
ملخص المقالة :
Restriction fragment length polymorphisms (RFLP) have been identified at the goat. The objective of the present study was to determine polymorphisms of calpastatin locus in Raini and Tali goats of the goats in Kerman Province, improving meat quality traits superior meat quality by selection. Calpastatin gene effect on quality and tenderness meat, to identify different genotype of this gene was randomly selected 150 Tali and 150 Raini goats, and blood samples were collected from total of animals using ethylenediaminetetraacetic acid (EDTA) tubes. DNA was extracted from blood according to DNA preparation kit to determine the genetic relationship among studied goat animals. Amplification was performed using polymerase chain reaction (PCR). Two alleles (A and B) and three genotypes, (AA, BB and AB) were observed. The frequencies of the observed genotypes for Tali and raini were 38.4, 9.6, 52 and 17, 71, 12 for AA, BB and AB, respectively. And allele frequencies were 0.47, 0.53 and 0.12, 0.88 for A and B, in Tali and Raini goats respectively.
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