Effect of Adding Coenzyme Q10 and Ellagic Acid during Cryopreservation on Post-Thaw Quality of Ram Semen
الموضوعات :ح. دقیقکیا 1 , ز. بلوکی 2 , ح. واثقی دودران 3 , م. مهدیپور 4
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
الکلمات المفتاحية: Q10, sperm, ram, ellagic acid,
ملخص المقالة :
The aim of this study was to determine the effects of co-Q10 and ellagic acid on sperm parameters after the freeze–thawing of Ghezel ram sperm. Twenty ejaculates from five Ghezel rams were collected in this study. Semen samples, which were diluted with a soybean lecithin (SL) based extender containing 0.25 mM ellagic acid, 0.5 μM co-Q10, 0.25 mM ellagic acid + 0.5 μM co-Q10 and no antioxidant (control), were cooled to 4˚C, frozen in 0.25 mL French straws and stored in liquid nitrogen. Sperm motility characteristics, membrane integrity, abnormal morphology, lipid peroxidation and antioxidant activities (glutathione peroxidase, superoxide dismutase and total antioxidant capacity) were evaluated following freeze-thawing. The results showed that 0.5 μM Co-Q10, improved viability, total motility parameters and decreased abnormal sperm and improved linearity (LIN), curvilinear velocity (VCL), straight-line velocity (VSL) and path velocity (VAP) parameters (P<0.05). Ellagic acid and the treatment with a combination (0.25 mM ellagic acid+0.5 μM co-Q10) improved viability and total motility parameters (P<0.05). The additives did not affect the maintenance of superoxide dismutase (SOD) and glutathione peroxidase (GPx), when compared to the control. It can be concluded that addition of 0.5 μM co-Q10 improved the post-thawing quality of ram semen.
Aitken R.J. and Sawyer D. (2003). The human spermatozoon-not waving but drowning. Adv. Exp. Med. Biol. 518, 85-98.
Alleva R., Scararmucci A., Mantero F., Bompadre S., Leoni L. and Littarru G. (1997). The protective role of ubiquinol-10 against formation of lipid hydroperoxides in human seminal fluid. Mol. Aspects. Med. 18, 221-228.
Almeida J. and Ball B.A. (2005). Effect of α-tocopherol and tocopherol succinate on lipid peroxidation in equine spermatozoa. Anim. Reprod. Sci. 87, 321-337.
Bailey J.L., Bilodeau J. and Cormier N. (2000). Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon. J. Androl. 21, 1-7.
Bondet V., Brand-Williams W. and Berset C. (1997). Kinetics and mechanisms of antioxidant activity using the DPPH. Free radical method. J. Food Sci. Technol. 30, 609-615.
Bucak M.N., Sarıözkan S., Tuncer P.B., Ulutaş P.A. and Akçadağ H.İ. (2009). Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation. Small Rumin. Res. 81, 90-95.
Çeribaşi A.O., Türk G., Sönmez M., Sakin F. and Ateşşahin A. (2010). Toxic effect of cyclophosphamide on sperm morphology, testicular histology and blood oxidant antioxidant balance and protective roles of lycopene and ellagic acid. Basic. Clin. Pharmacol. Toxicol. 107, 730-736.
Daghigh Kia H., Farhadi R., Ashrafi I. and Mehdipour M. (2016a). Anti oxidative effects of ethanol extract of Origanum vulgare on kinetics, microscopic and oxidative parameters of cryopreserved Holstein bull spermatozoa. Iranian J. Appl. Anim. Sci. 6, 901-907.
Daghigh Kia H., Razavian S., Dodran H.V. and Najafi A. (2016b). Effect of adding Rosa canina extract and ascorbic acid as natural and synthetic antioxidants on freeze-thawing process of ram semen. Anim. Rep. 13, 567-567.
Daghigh Kia H., Shahbaz Zadeh R. and Ashrafi I. (2015). Effect of antioxidant Satureja makranta the microscopic and biochemical parameters of bull sperm after freezing - thawing process. J. Anim. Sci. 108, 101-112.
Ernster L. (1993). Lipid peroxidation in biological membranes: Mechanisms and implications. Pp. 1-38 in Active Oxygens, Lipid Peroxides, and Antioxidants. K. Yagi, Ed. CRC Press, Boca Raton, Florida. USA.
Ernster L. and Dallner G. (1995). Biochemical, physiological and medical aspects of ubiquinone function. Biochim. Biophys. Acta (BBA)-Mol. Basis Dis. 1271, 195-204.
Eskenazi B., Kidd S., Marks A., Sloter E., Block G. and Wyrobek A. (2005). Antioxidant intake is associated with semen quality in healthy men. Hum. Reprod. 20, 1006-1012.
Esterbauer H., Dieber-Rotheneder M., Waeg G., Striegl G. and Juergens G. (1990). Biochemical structural and functional properties of oxidized low-density lipoprotein. Chem. Res. Toxicol. 3, 77-92.
Funahashi H. and Sano T. (2005). Select antioxidants improve the function of extended boar semen stored at 10 C. Theriogenology. 63, 1605-1616.
Hassoun E.A., Vodhanel J. and Abushaban A. (2004). The modulatory effects of ellagic acid and vitamin E succinate on TCDD induced oxidative stress in different brain regions of rats after subchronic exposure. J. Biochem. Mol. Toxicol. 18, 196-203.
Jeyendran R.S., Van der Ven H.H., Perez-Pelaez M., Crabo B.G. and Zaneveld L.J. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to the other sperm characteristics. J. Reprod. Fertil. 70, 219-28.
Lewin A. and Lavon H. (1997). The effect of coenzyme Q10 on sperm motility and function. Mol. Aspects. Med. 18, 213-219.
Lee W., Li K. and Huang Y. (2006). Biological function of CoQ10 and its effect on the quality of spermatozoa. Zhonghua Nan. Ke. Xue. 12, 1119-1122.
Mancini A., Conte G., Milardi D., De Marinis L. and Littarru G. (1998). Relationshipbetween sperm cell iquinone and seminal parameters in subjects with and without varicocele. Andrologia. 30, 1-4.
Mansour S.W., Sangi S., Harsha S., Khaleel M.A. and Ibrahim A. (2013). Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract. Asian Pac. J. Trop. Biomed. 3, 563-568.
Mata-Campuzano M., Alvarez-Rodriguez M., Alvarez M., Tamayo-Canul J., Anel L., de Paz P. and Martinez-Pastor F. (2015). Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology. 83, 520-528.
Mehdipour M., Daghigh K.I.A.H., Najafi A., Vaseghi-Dodaran H. and García-Álvarez O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 73, 297-303.
Michael A., Alexopoulos C., Pontiki E., Hadjipavlou-Litina D., Saratsis P. and Boscos C. (2007). Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology. 68, 204-212.
Miller N.J., Rice-Evans C., Davies M.J., Gopinathan V. and Milner A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. 84(4), 407-412.
Najafi A., Daghigh Kia H., Mohammadi H., Najafi M.H., Zanganeh Z., Sharafi M., Martinez-Pastor F. and Adeldust H. (2014a). Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology. 69, 68-73.
Najafi A., Najafi M.H., Zanganeh Z., Sharafi M., Martinez-Pastor F. and Adeldust H. (2014b). Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant. Reprod. Domest. Anim. 49, 934-940.
Najafi A., Zhandi M., Towhidi A., Sharafi M., Akbari Sharif A., Khodaei Motlagh M. and Martinez-Pastor F. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 66, 275-282.
Najafi A., Daghigh-Kiaa H., Vaseghi Dodarana H., Mehdipoor M. and Alvarez-Rodriguez A. (2016). Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation. Anim. Reprod. Sci. 177, 35-41.
Omur A. and Coyan K. (2016). Protective effects of the antioxidants curcumin, ellagic acid and methionine on motility, mitochondrial transmembrane potential, plasma membrane and acrosome integrity in freeze-thawed Merino ram sperm. Vet. Med. 61, 10-16.
Omur A., Coyan K., Ozturk C., Gungor S. and Bucak M. (2014). The effects of curcumin, ellagic acid and methionine on post-thawed Merino rams sperm parameters (759.1). FASEB J. 28, 751-759.
Placer Z.A., Cushman L.L. and Johnson B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal. Biochem. 16, 359-364.
Priyadarsini K.I., Khopde S.M., Kumar S.S. and Mohan H. (2002). Free radical studies of ellagic acid, a natural phenolic antioxidant. J. Agric. Food Chem. 50, 2200-2206.
Reda El-Sheshtawy I., Gamal El-Sisy A. and Walid El-Nattat S. (2016). Effects of pomegranate juice in Tris-based extender on cattle semen quality after chilling and cryopreservation. Asian Pac. J. Reprod. 4(1), 26-31.
Salamon S. and Maxwell W. (2000). Storage of ram semen. Anim. Reprod. Sci. 62, 77-111.
Sariozkan S., Tuncer P.B., Buyukleblebici S., Bucak M.N., Canturk F. and Eken A. (2015). Antioxidative effects of cystea-mine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull. Andrologia. 47, 138-147.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Seeram N.P., Adams L.S., Henning S.M., Niu Y., Zhang Y., Nair M.G. and Heber D. (2005). In vitro anti-proliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J. Nutr. Biochem. 16, 360-367.
Sheweita S.A., Tilmisany A.M. and Al-Sawaf H. (2005). Mechanisms of male infertility: role of antioxidants. Curr. Drug Metab. 6, 495-501.
Turunen M., Olsson J. and Dallner G. (2004). Metabolism and function of coenzyme Q. Biochim. Biophys. Acta. 1660(2), 171-199.
Türk G., Ateşşahin A., Sönmez M., Çeribaşi A.O. and Yüce A. (2008). Improvement of cisplatin-induced injuries to sperm quality, the oxidant-antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertil. Steril. 89, 1474-1481.
Vaseghi Dodaran H., Zhandi M., Sharafi M., Nejati-Amiri E., Nejati-Javaremi A., Mohammadi-Sangcheshme A., Shehab-El-Deen M.A.M. and Shakeri M. (2015). Effect of ethanol induced mild stress on post-thawed bull sperm quality. Cryobiology. 71, 12-17.
Yousefian I., Zare-Shahneh A. and Zhandi M. (2014). The effect of coenzyme Q10 and α-tocopherol in skim milk-based extender for preservation of Caspian stallion semen in cool condition. J. Equine Vet. Sci. 34, 949-954.
