Relationship of Mitochondrial Biogenesis and Phenotypic Expression of Residual Feed Intake in Fat-Tailed Lambs
الموضوعات :
H. Rajaei-Sharifabadi
1
(Department of Animal Science, Faculty of Agriculture, Malayer University, Malayer, Iran)
A.A. Naserian
2
(Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
M.R. Nassiry
3
(Recombinant Protein Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran)
W. Bottje
4
(Department of Poultry Science, Center of Excellence for Poultry Science, Division of Agriculture, University of Arkansas, Arkansas, USA)
الکلمات المفتاحية: feed efficiency, mitochondrial DNA, skeletal muscle, PGC-1α, SIRT1,
ملخص المقالة :
Residual feed intake (RFI) is a popular measure of feed efficiency in livestock animals. The functionality of mitochondrial metabolism has been shown to be associated with variation in feed efficiency. In this study, mitochondrial biogenesis was determined by measuring the mitochondrial DNA (mtDNA) copy number in skeletal muscle of two groups of fat-tailed lambs divergently ranked on phenotypic expression of RFI (high- and low-RFI). A quantitative real-time PCR was performed to determine the relative mRNA levels of peroxisome proliferator-activated receptor γ coactivator 1 α (PGC-1α) and NAD+-depended deacetylase sirtuin1 (SIRT1), as two major genes involved in the regulation of mitochondrial biogenesis. The level of mtDNA in skeletal muscle of low-RFI lambs was higher than those ranked as high-RFI. Higher mRNA level of PGC-1α was also detected in low-RFI lambs in compared with the high-RFI group. However, no significant difference was observed between the RFI groups for mRNA expression of SIRT1. The results showed a negative relationship between phenotypic expression of RFI with mitochondrial biogenesis and PGC-1α mRNA level in skeletal muscle of fat-tailed lambs. Overally, our results suggest that the increased mitochondrial dynamics has a potential to regulates bioenergetic efficiency and energy expenditure in low-RFI lambs.
Addabbo F., Ratliff B., Park H.C., Kuo M.C., Ungvari Z., Ciszar A., Krasnikof B., Sodhi K., Zhang F., Nasjletti A. and Goligorsky M.S. (2009). The krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: Proteomic approach. Am. J. Pathol. 174(1), 34-43.
Anderson S., Bankier A.T., Barrell B.G., de Bruijn M.H.L., Coulson A.R., Drouin J., Eperon I.C., Nierlich D.P., Roe B.A., Sanger F., Schreier P.H., Smith A.J.H., Staden R. and Young I.G. (1981). Sequence and organization of the human mitochondrial genome. Nature. 290, 457-465.
Archer J.A., Richardson E.C., Herd R.M. and Arthur P.F. (1999). Potential for selection to improve efficiency of feed use in beef cattle: A review. Australian J. Agric. Res. 50(2), 147-162.
Arthur P.F., Archer J.A., Johnston D.J., Herd R.M., Richardson E.C. and Parnell P.F. (2001). Genetic and phenotypic variance and covariance components for feed intake, feed efficiency, and other postweaning traits in angus cattle. J. Anim. Sci. 79(11), 2805-2811.
Austin S. and St-Pierre J. (2012). Pgc1α and mitochondrial metabolism – emerging concepts and relevance in ageing and neurodegenerative disorders. J. Cell Sci. 125(21), 4963-4971.
Bottje W. and Carstens G.E. (2009). Association of mitochondrial function and feed efficiency in poultry and livestock species. J. Anim. Sci. 87(14), 48-63.
Bottje W. and Kong B.W. (2013). Feed efficiency: Mitochondrial function to global gene expression. J. Anim. Sci. 91(4), 1582-1593.
Bottje W., Iqbal M., Tang Z.X., Cawthon D., Okimoto R., Wing T. and Cooper M. (2002). Association of mitochondrial function with feed efficiency within a single genetic line of male broilers. Poult. Sci. 81(4), 546-555.
Ceacero T.M., Mercadante M.E., Cyrillo J.N., Canesin R.C., Bonilha S.F. and de Albuquerque L.G. (2016). Phenotypic and genetic correlations of feed efficiency traits with growth and carcass traits in nellore cattle selected for postweaning weight. PLoS One. 11, e0161366.
Davinelli S., Sapere N., Visentin M., Zella D. and Scapagnini G. (2013). Enhancement of mitochondrial biogenesis with polyphenols: Combined effects of resveratrol and equol in human endothelial cells. Immun. Ageing. 10(1), 28-33.
De Haer L.C.M., Luiting P. and Aarts H.L.M. (1993). Relations among individual (residual) feed intake, growth performance and feed intake pattern of growing pigs in group housing. Livest. Prod. Sci. 36(3), 233-253.
Dervishi E., Serrano C., Joy M., Serrano M., Rodellar C. and Calvo J.H. (2010). Effect of the feeding system on the fatty acid composition, expression of the δ9-desaturase, peroxisome proliferator-activated receptor alpha, gamma, and sterol regulatory element binding protein 1 genes in the semitendinous muscle of light lambs of the rasa aragonesa breed. BMC Vet. Res. 6(1), 40-49.
Durunna O.N., Plastow G., Mujibi F.D., Grant J., Mah J., Basarab J.A., Okine E.K., Moore S.S. and Wang Z. (2011). Genetic parameters and genotype × environment interaction for feed efficiency traits in steers fed grower and finisher diets. J. Anim. Sci. 89(11), 3394-3400.
Fernandez-Marcos P.J. and Auwerx J. (2011). Regulation of pgc-1alpha, a nodal regulator of mitochondrial biogenesis. Am. J. Clin. Nutr. 93(4), 884-890.
Fonseca L.F., Gimenez D.F., Mercadante M.E., Bonilha S.F., Ferro J.A., Baldi F., de Souza F.R. and de Albuquerque L.G. (2015). Expression of genes related to mitochondrial function in nellore cattle divergently ranked on residual feed intake. Mol. Biol. Rep. 42(2), 559-565.
Herd R.M. and Arthur P.F. (2009). Physiological basis for residual feed intake. J. Anim. Sci. 87(14), 64-71.
Kelly A.K., Waters S.M., McGee M., Fonseca R.G., Carberry C. and Kenny D.A. (2011). Mrna expression of genes regulating oxidative phosphorylation in the muscle of beef cattle divergently ranked on residual feed intake. Physiol. Genom. 43(1), 12-23.
Kolath W.H., Kerley M.S., Golden J.W. and Keisler D.H. (2006). The relationship between mitochondrial function and residual feed intake in angus steers. J. Anim. Sci. 84(4), 861-865.
Kong B.W., Lassiter K., Piekarski-Welsher A., Dridi S., Reverter-Gomez A., Hudson N.J. and Bottje W.G. (2016a). Proteomics of breast muscle tissue associated with the phenotypic expression of feed efficiency within a pedigree male broiler line: I. Highlight on mitochondria. PLOS ONE 11(5), e0155679.
Kong R.S.G., Liang G., Chen Y., Stothard P. and Guan L.L. (2016b). Transcriptome profiling of the rumen epithelium of beef cattle differing in residual feed intake. BMC Genom. 17(1), 592-601.
Livak K.J. and Schmittgen T.D. (2001). Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method. Methods 25(4), 402-408.
Nisoli E., Clementi E., Paolucci C., Cozzi V., Tonello C., Sciorati C., Bracale R., Valerio A., Francolini M., Moncada S. and Carruba M.O. (2003). Mitochondrial biogenesis in mammals: The role of endogenous nitric oxide. Science. 299, 896-899.
Rajaei Sharifabadi H., Naserian A.A., Valizadeh R., Nassiry M.R., Bottje W.G. and Redden R.R. (2016). Growth performance, feed digestibility, body composition, and feeding behavior of high- and low-residual feed intake fat-tailed lambs under moderate feed restriction. J. Anim. Sci. 94(8), 3382-3388.
Rajaei Sharifabadi H., Zamiri M.J., Rowghani E. and Bottje W.G. (2012). Relationship between the activity of mitochondrial respiratory chain complexes and feed efficiency in fat-tailed ghezel lambs. J. Anim. Sci. 90(6), 1807-1815.
Rodgers J.T., Lerin C., Haas W., Gygi S.P., Spiegelman B.M. and Puigserver P. (2005). Nutrient control of glucose homeostasis through a complex of pgc-1alpha and sirt1. Nature 434, 113-118.
Sambrook J., Maniatis T. and Fritsch E.F. (1987). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
SAS Institute. (2004). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Tizioto P.C., Coutinho L.L., Decker J.E., Schnabel R.D., Rosa K.O., Oliveira P.S., Souza M.M., Mourão G.B., Tullio R.R., Chaves A.S., Lanna D.P., Zerlotini-Neto A., Mudadu M.A., Taylor J.F. and Regitano L.C. (2015). Global liver gene expression differences in nelore steers with divergent residual feed intake phenotypes. BMC Genom. 16(1), 242-250.
Ventura-Clapier R., Garnier A. and Veksler V. (2008). Transcriptional control of mitochondrial biogenesis: The central role of pgc-1alpha. Cardiovasc. Res. 79(2), 208-217.
Zamiri M.J., Mehrabi R., Kavoosi G.R. and Sharifabadi H.R. (2016). Relationships between the activity of respiratory-chain complexes in pre- (biopsy) or post-slaughter muscle samples and feed efficiency in random-bred ghezel lambs. Anim. Prod. Sci. 57(8), 1674-1681.