Comparison of in vitro Fermentation with in situ Degradation to Estimate Dry Matter Degradability and Energy Protein Synchronization of Roughage Based Diets
الموضوعات :م. آیونگ 1 , ی.ی. کیاوت 2 , م.ت. هتون 3 , ک.س. میو 4 , آ. آیونگ 5
1 - Department of Animal Nutrition, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar
2 - Department of Animal Nutrition, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar
3 - Department of Physiology and Biochemistry, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmara
4 - Department of Animal Nutrition, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmar
5 - Department of Physiology and Biochemistry, University of Veterinary Science, Yezin, Nay Pyi Taw, 15013, Myanmara
الکلمات المفتاحية: Correlation, degradability, synchronization, <i>in situ</i> degradation, <i>in vitro</i> fermentation,
ملخص المقالة :
This study was aimed to evaluate the correlation between in vitro fermentation and in situ degradation parameters and to predict dry matter degradability and energy protein synchronization of roughage based diets. Different inclusion of roughage in diets [roughage 50% diet (R50D), roughage 60% diet (R60D), roughage 65% diet (R65D) and roughage 70% diet (R70D)] were used to determine in vitro and in situ parameters. The relationships between in vitro and in situ parameters were analyzed by simple linear regression. The gas volumes and fermentation kinetics of R50D and R65D were greater (P<0.05) than those of other diets, however the lesser values of partitioning factor for microbial protein synthesis efficiency was found in R50D. Although the nutrient disappearances, degradation kinetics and effective degradability were greater (P<0.05) in R50D in compare with R60D and R65D, the lowest (P<0.05) energy protein synchronization was observed in R50D. The significant correlation (P<0.05) were observed in all regression equations of in vitro gas volumes and in situ nutrient disappearances. The in situ effective dry matter degradability and energy protein synchronization were correlated (P<0.05) with in vitro fermentation kinetics and some estimated parameters such as short chain fatty acid and partitioning factor for microbial protein synthesis efficiency. Among the correlations, the greater accuracy could be achieved by inclusion of two or more parameters in regression equation. The results showed that in vitro gas production technique has the potential to predict effective dry matter degradability and energy protein synchronization of roughage based diets.
Adesogan A.T. (2002). What are feeds worth? A critical evaluation of selected nutritive value methods. Pp. 33-47 in Proc. 13th Ann. Florida Rumin. Nutr. Symp., Gainesville, Florida.
Aiple K.P., Steingass H. and Drochner W. (1996). Prediction of the net energy content of raw materials and compound feeds for ruminants by different laboratory methods. Arch. Anim. Nutri. 49, 213-220.
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Blümmel M. and Ørskov E.R. (1993). Comparison of in vitro gas production and nylon bag degradability of roughages in predicting of food intake in cattle. Anim. Feed Sci. Technol. 40, 109-119.
Blümmel M., Mgomezulu R., Chen X.B., Makkar H.P.S., Becker K. and Ørskov E.R. (1999). The modification of in vitro gas production test to detect roughage related differences in in vivo microbial protein synthesis as estimated by the excretion of purine derivate. J. Agric. Sci. 133, 335-340.
Blümmel M., Ørskov E.R., Becker K. and Soller H. (1990). Application of the Hohenheim gas test for evaluating the kinetics of rumen fermentation. J. Anim. Physiol. Anim. Nutr. 64, 56-57.
Cole N.A. and Todd R.W. (2008). Opportunities to enhance performance and efficiency through nutrient synchrony in concentrate-fed ruminants. J. Anim. Sci. 86, 318-333.
Cone J.W., Van Gelder A.H., Bachmann H. and Hindle V. (2002). Comparison of organic matter degradation in several feedstuffs in the rumen as determined with the nylon bag and gas production techniques. Anim. Feed Sci. Technol. 96, 55-67.
Deaville E.R. and Givens D.I. (2001). Use of the automated gas production technique to determine the fermentation kinetics of carbohydrate fractions in maize silage. Anim. Feed Sci. Technol. 93, 205-215.
Elseed F.A.M.A. (2005). Effect of supplemental protein feeding frequency on ruminal characteristics and microbial N production in sheep fed treated rice straw. Small Rumin. Res. 57, 11-17.
Getachew G., Grovetto G.M., Fondivilla M., Krishnamoorthy B., Singh Sphaghero H., Steingass P.H., Robinson P.H. and Kailas M.M. (2002). Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy values of ruminant feeds. Anim. Feed Sci. Technol. 102, 169-180.
Getachew G., Makkar H.P.S. and Becker K. (1999). Stoichiometric relationship between short chain fatty acids and in vitro gas production in presence and polythyleneglycol for tannin containing browses. Pp. 55-61 in Proc. EAAP Satellite Symp. Gas Prod., Wageningen, The Netherlands.
Goering K.H. and van Soest P.J. (1970). Forage Fibre Analysis. Agricultural Hand Book, Washington, D.C., USA.
Kamalak A., Canbolat O., Gurbuz Y. and Ozay O. (2005). Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation. Czech J. Anim. Sci. 50(2), 60-67.
Khazaal K., Markantonatos X., Nastis A. and Ørskov E.R. (1993). Changes with maturity in fibre composition and levels of extractable polyphenols in Greek browse: effect in vitro gas production and in sacco dry matter degradation. J. Sci. Food Agric. 63, 237-244.
Kotarski S.F., Waniska R.D. and Thurn K.K. (1992). Starch hydrolysis by the ruminant micro flora. J. Nutr. 122, 178-190.
Krishna G. and Gunther K.D. (1987). The usability Hohenheim gas test for evaluation in vitro organic matter digestibility and protein degradability at rumen level of some agro industrial by-products and wastes used as livestock feeds. Landwirtsch. Forx. 40, 281-286.
Krishnamoorthy H., Steingass H. and Menke K.H. (1991). Preliminary observations on the relationships between gas production and microbial protein synthesis in vitro. Arch. Tierernahr. 41, 521-526.
Krishnamoorthy U., Soller H., Steingass H. and Menke K. (1995). Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analysis and rumen inoculums studies in vitro. Anim. Feed Sci. Technol. 52, 177-188.
Lardy G.P., Ulmer D.N., Anderson V.L. and Caton J.S. (2004). Effect of increasing level of supplemental barley on forage intake, digestibility, and ruminal fermentation in steers fed medium quality grass hay. J. Anim. Sci. 82, 3662-3668.
Maghsoud B., Akbar T., Hossein J. and Ali M.G. (2008). Evaluation of some by-products using in situ and in vitro gas production techniques. American J. Anim. Vet. Sci. 3(1), 7-12.
Makkar H.P.S. (2005). In vitro gas methods for evaluation of feeds containing phytochemicals. Anim. Feed Sci. Technol. 123, 291-302.
Makkar H.P.S. and Becker K. (1996). Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Anim. Feed Sci. Technol. 63, 211-228.
Makkar H.P.S., Aregheore E.M. and Becker K. (1999). Effect of saponins and plant extracts containing saponins on the recovery of ammonia during urea-ammoniation of wheat straw and fermentation kinetics of the treated straw. J. Agric. Sci. 132, 313-321.
Mehrez A.Z. and Ørskov E.R. (1977). A study of artificial fibre bag technique for determining the digestibility of feeds in the rumen. J. Agric. Sci. 88, 645-650.
Menke K.H. and Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7-55.
Menke K.H., Raab L., Salewski A., Steingass H., Fritz D. and Schneider W. (1979). The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor. J. Agric. Sci. 93, 217-222.
Ørskov E.R. (1989). Recent advances in evaluation of roughages as feeds for ruminants. Pp. 102-108 in Advances in Animal Nutrition. D.J. Farell, Ed. University of New England Printery, Armidale, Australia.
Ørskov E.R. and McDonald P. (1979). The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. J. Agric. Sci. 92, 499-503.
Ozkan C.O. and Sahin M. (2006). Comparison of in situ dry matter degradation with in vitro gas production of Oak leaves supplemented with or without polyethylene glycol (PEG). Asian-Australasian J. Anim. Sci. 19(8), 1120-1126.
Sallam S.M.A., Bueno I.C.S., Godoy P.B., Nozella E.F., Vitti D.M. and Al-Abdalla S.S. (2008). Nutritive value in the value assessment of the artichoke (Cynara scolyms) by products as an alternative feed resource for ruminant. Trop. Subtrop. Agroeco. 8, 181-189.
Sileshi Z., Owen E., Dhanona M.S. and Theodorou M.K. (1996). Prediction of in situ rumen dry matter disappearance of Ethiopian forages from an in vitro gas production technique using a pressure transducer, chemical analyses or in vitro digestibility. Anim. Feed Sci. Technol. 61, 73-77.
Sinclair L.A., Garnsworth P.C., Newbold J.R. and Buttery P.J. (1993). Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. J. Agric. Sci. 120, 251-263.
SPSS Inc. (2007). Statistical Package for Social Sciences Study. SPSS for Windows, Version 16. Chicago SPSS Inc., USA.
Steel R.G. and Torrie J.H. (1980). Principles and Procedures of Statistics. McGraw-Hill Book Company, New York.
Valentin S.F., Williams P.E.V., Forbes J.M. and Sauvant D. (1999). Comparison of the in vitro gas production technique and the nylon bag degradability technique to measure short and long term processes of degradation of maize silage in dairy cows. Anim. Feed Sci. Technol. 78, 81-99.