Using elicitors for enhanced production of secondary metabolites in plant cell and organ suspension cultures
الموضوعات : مجله گیاهان دارویی
مریم محمدی فارسانی
1
(دانشگاه پیام نور تهران شرق، ایران)
عبدالله قاسمی پیربلوطی
2
(مرکز پژوهش های گیاهان دارویی و دام پزشکی سنتی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران)
الکلمات المفتاحية: elicitor, Secondary metabolites, signaling pathway, Plant Cell Culture,
ملخص المقالة :
In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those found in whole plants. Various strategies have been employed to increase the production of secondary metabolites in cell cultures. These include manipulation of culture media (hormonal and nutrient stress) and environmental conditions (temperature, pH and osmotic stress), precursor addition, elicitation and combination of these strategies. Nowadays, genetic manipulation of biosynthetic pathways by metabolic engineering has also become a powerful technique for enhanced production of desired metabolites. Studies have shown that both biotic and abiotic elicitors synthesis of secondary metabolites increases in plant cell culture medium.
Bonfante, P. 2009. Symbiotic fungi: principles and practice. vol. 18. Springer Heidelberg Dordrecht London New York. pp 373
Bourgaud, F., Gravot, A., Milesi, S. and Gontier, E. 2001. Production of plant secondary metabolites: a historical perspective. Plant Science, 161: 839-851.
Box, G.E.P. 1945. The exploration and exploitation of response surfaces: some general considerations and examples. Biometrics, 10: 16-60.
Dixon, R.A. 2001. Natural products and plant disease resistance. Nature, 411(6839): 843-847.
Hadimani, S.B., Tanpure, R.P. and Bhat, S.V. 1996. Asymmetric total synthesis of (-) Podophyllotoxin. Tetrahedron Lett, 37: 4791–4794.
Imbert, F. 1998. Discovery of podophyllotoxins. Biochimie, 80: 207-222.
Li, J., Ou-Lee, T.M., Raba, R., Amundson, R.G. and Last, R.L. 1993. Arabidopsis mutants are hypersensitive to UV-B radiation. Plant Cell, 5: 171–179.
Oksman-Caldentey, K. M., and Inze, D., 2004. Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends Plant Sci, 9: 433-440.
Patel, H. and Krishnamurthy, R. 2013. Elicitors in Plant Tissue Culture. Pharmacognosy and Phytochemistry, 2: 60-65.
Pichersky, E. and Gang, D.R. 2000. Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends in plant science, 5: 439-445.
Rao, R.S. and Ravishankar, G.A. 2002. Plant tissue cultures; chemical factories of secondary metabolites. Biotechnol, 20: 101-153.
Stahelin, H.F. and Wartburg, A.V. 1991. The chemical and biological route from podophyllotoxin glucoside to etoposide. Cancer Res, 51: 5-15.
Vasconsuelo, A. and Boland, R. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci, 172: 861-875.
Wink, M., Alfermann, A.W., Franke, R., Wetterauer, B., Distl, M., Windhovel, J., Krohn, O., Fuss, E., Garden, H., Mohagheghzaden, A., Wildi, E. and Ripplinger, P. 2005. Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents. Plant Genetic Resour, 3: 90-100.
Zhao, J., Davis, L.C. and Verpoorte, R. 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv, 23: 283-333.
Zhoua, L.G. and Wu, J.U. 2006. Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China. Nat Prod Rep, 23: 789–810.
