Anti-proliferative effect of Trifolium pratense extract on Raji cell line in Burkitt's lymphoma
الموضوعات : مجله گیاهان دارویی
مرتضی داودی
1
,
شهریار سعیدیان
2
,
رضا صغیری
3
,
زهرا زمانی
4
,
غلام رضا بخشی خانیکی
5
1 - گروه بیوشیمی، دانشگاه پیام نور، تهران، ایران;
2 - گروه بیوشیمی، دانشگاه پیام نور، تهران، ایران;
3 - انستیتو پاستور ایران، تهران، ایران;
4 - انستیتو پاستور ایران، تهران، ایران;
5 - گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران;
الکلمات المفتاحية: ID3, Red Clover, p53, Burkitt's lymphoma, c-MYC, Raji cells,
ملخص المقالة :
Background & Aim: In folk medicine, red clover (Trifolium pratense) use to treat some diseases such as whooping cough, asthma, eczema, and some ophthalmic dysfunction. Due to its phytoestrogens and antioxidant-rich compounds, it is recommended to alleviate some cancers. This paper was conducted to study the anti-cancer and apoptosis effects of red clover extract (RCE) on the Raji cell line in Burkitt's lymphoma.Experimental: Red clover lyophilized powder (CAS = 85085-25-2) with a fixed ratio of Formononetin, Biochanin A, Daidzein, and Genistein was acquired from Sigma Aldrich German company. Raji cells were obtained from the Pasteur Institute of Iran-Tehran. Raji cells were treated with different concentrations of RCE, and viable cells were measured at an interval of 24, 48, and 72 hours incubation in three days by MTT assay. Agarose gel electrophoresis was used to determine the DNA degradation. Annexin V-FITC/PI kit and flow cytometry assay were used to determine the percent of apoptotic and necrotic treated Raji cells. Also, expression changes incMYC,ID3, andP53genes were measured by real-time PCR.Results: Red clover extract can prevent in vitro proliferation of the Raji cells in a time and dose-dependent manner. RCE, as well asc-MYCgene suppression and induction ofID3andP53genes, enters the Raji cells in the apoptosis stages with an acceptable percentage and has complication cure potential in Burkitt's lymphoma.Recommended applications/industries: The extract used in this studycombines four components. The individual evaluation effects ofFormononetin, Biochanin A, Daidzein, and Genistein on Raji cells are recommended.
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