ویژگی ضد باکتریایی نانو ذرات نقره/کلرید نقره بیوسنتزی با استفاده از عصاره برگ‌گیاه مرزه تحت تاثیر پرتو‌گاما
الموضوعات :
1 - استادیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی، قزوین، ایران.
الکلمات المفتاحية: پرتو گاما, E. coli, فعالیت ضد باکتری, B. subtilis, عصاره گیاه مرزه,
ملخص المقالة :
یکی از مهمترین مشکلات بهداشتی در سراسر جهان افزایش میکروارگانیسم‌های بیماری زا‌ی مقاوم به انواع مواد ضدمیکروبی است. در این پژوهش ویژگی ضدباکتریایی نانو ذرات نقره/کلرید نقره بیوسنتزی توسط عصاره برگ گیاه دارویی مرزه تحت تاثیر دوزهای مختلف پرتو گاما مقایسه شده است. بیوسنتز نانو ذرات نقره/کلرید نقره توسط عصاره برگ گیاهان روئیده از بذرهایی که تحت تاثیر دوزهای مختلف از اشعه گاما (0، 15، 30، 60 و 90 گری) قرار گرفته بودند، انجام شد. تشکیل نانو ذرات بیوسنتزی توسط دستگاه‌های اسپکتروفوتومتری UV و XRD تایید شده و ویژگی‌های آن‌ها مانند میانگین اندازه توسط DLS، شکل و اندازه توسط FESEM، آنالیز عنصری توسط EDAX، تعیین گروه‌های عاملی عصاره گیاه موثر در فرایند توسط FTIR و ویژگی ضدباکتریایی آن‌ها به روش انتشار دیسک (Disc Diffusion) انجام شد. بیشترین خاصیت ضدباکتریایی نانو ذرات نقره/کلرید نقره بیوسنتزی توسط دوز 15 گری از گاما با میانگین اندازه نانوذرات nm 02/40 و قطر هاله بازدارندگی رشد mm13 بر ضد باکتری Escherichia coli و کمترین آن در دوز 90 گری با میانگین اندازه نانوذرات nm 62/170 و قطر هاله بازدارندگی رشد mm10 بر ضد همین باکتری مشاهده شد. نانوذرات نقره بیوسنتزی توسط عصاره گیاه مرزه تیمار شده با دوز 15 گری از پرتو گاما، بیشترین ویژگی ضد باکتریایی را بر علیه باکتری Bacillus subtilis و به ویژه E. coli نشان داد. زیست سازگاری بالای این نانو ذرات بیوسنتزی، آن‌ها را جایگزین مناسبی به جای مواد مشابه سنتز شده از روش‌های غیر زیستی نشان می‌دهد.
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