پاسخ کاسپاز 3 و ظرفیت تام آنتی اکسیدانی به مصرف دارچین و تمرین اینتروال در موشهای صحرایی نر
محورهای موضوعی : زیست شناسی جانوری
1 - گروه فیزیولوژی ورزشی، واحد ورامین -پیشوا، دانشگاه آزاد اسلامی، ورامین ، ایران
2 - گروه فیزیولوژی ورزشی، واحد ورامین -پیشوا، دانشگاه آزاد اسلامی، ورامین ، ایران
کلید واژه: دارچین , تمرین اینتروال , کاسپاز 3 , TAC , آنتی اکسیدان,
چکیده مقاله :
مقدمه: استرس اکسیداتیو و آپوپتوز سلولی از مهم‌ترین مکانیسم‌های تخریب بافتی به‌شمار می‌روند. مداخلات تغذیه‌ای و فعالیت‌های ورزشی ممکن است اثراتی بر این شاخص‌ها داشته باشند. هدف از این مطالعه بررسی اثر یک دوره مصرف دارچین و تمرین اینتروال شنا با شدت متوسط بر سطوح کاسپاز-3 و ظرفیت تام آنتی اکسیدانی در رت‌های نر بود.
مواد و روش ها: در این پژوهش تجربی، 40 موش صحرایی نر ویستار به‌طور تصادفی به چهار گروه: کنترل، عصاره دارچین، تمرین اینتروال شنا، و ترکیبی (عصاره دارچین+تمرین) تقسیم شدند. پروتکل تمرین شامل شنای اینتروال با شدت متوسط ( 5 جلسه در هفته به‌مدت 6 هفته) و مصرف دارچین ( دوز 200 میلی‌گرم بر کیلوگرم وزن بدن و غلظت 100 mg/mL) به صورت گاواژ روزانه بود. در پایان دوره، نمونه‌های سرمی از قلب اخذ و برای اندازه‌گیری سطوح کاسپاز-3 و ظرفیت تام آنتی اکسیدانی، با استفاده از روش ELISA مورد تحلیل قرار گرفتند. داده‌ها با آزمون‌های میانگین، انحراف استاندارد، تحلیل واریانس دو طرفه و آزمون تعقیبی توکی بررسی شدند (P<0/05).
یافته‌ها: نتایج نشان داد که در گروه ترکیبی، سطح کاسپاز-3 نسبت به گروه کنترل و سایر گروه‌ها کاهش معنادارداشته است و ظرفیت تام آنتی اکسیدانی در گروه های مداخله افزایش معنادار داشته اند (P<0/05). اندازه اثر نشان داد در گروه تعاملی هم افزایی اثرات مشاهده می شود.
نتیجه‌گیری: یافته‌های پژوهش نشان داد ترکیب مصرف عصاره دارچین و تمرین اینتروال با شدت متوسط شنا می‌تواند هم‌زمان با کاهش فرآیندهای آپوپتوتیک از طریق مهار کاسپاز-3، موجب ارتقای ظرفیت دفاع آنتی‌اکسیدانی بدن گردد. این نتایج می‌تواند مبنایی برای طراحی مداخلات پیشگیرانه یا درمانی مبتنی بر ترکیب فعالیت بدنی و مکمل‌های گیاهی در شرایط استرس اکسیداتیو باشد.
Introduction: Oxidative stress and cell apoptosis are considered to be the most important mechanisms of tissue damage. Nutritional interventions and exercise activities may have effects on these indicators. This study aimed to investigate the effect of a period of cinnamon consumption and moderate-intensity interval swimming training on caspase-3 and TAC levels in male rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were randomly divided into four groups: control, cinnamon, interval swimming training, and combination (cinnamon + interval swimming training). The training protocol included moderate-intensity interval swimming (5 sessions per week for 6 weeks) and cinnamon consumption (dose 200 mg/kg body weight) as daily gavage. At the end of the period, serum samples were collected from the heart and analyzed using ELISA to measure caspase-3 levels and total antioxidant capacity. Data were analyzed using mean, standard deviation, two-way analysis of variance, and Tukey's post hoc test (P<0.05).
Results: The results showed that in the combined group, caspase-3 levels decreased significantly compared to the control and other groups, and TAC increased significantly in the intervention groups (P<0.05). The effect size showed that synergistic effects were observed in the interaction group.
Conclusion: The findings of the study showed that the combination of cinnamon consumption and moderate-intensity interval swimming training can simultaneously reduce apoptotic processes through caspase-3 inhibition and improve the body's antioxidant defense capacity. These results can be a basis for designing preventive or therapeutic interventions based on the combination of physical activity and herbal supplements in conditions of oxidative stress.
Keywords: Cinnamon, Interval Training, Caspase 3, TAC, Antioxidant
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