بررسی مقایسه ای سرم آمیلوئید A در گاوهای مبتلا به ورم پستان تحت بالینی ناشی از عفونت ایشیریشیا کلی و استافیلوکوکوس اورئوس
محورهای موضوعی : پژوهش های بالینی و آزمایشگاهی دامپزشکی
ام البنین قاسمیان
1
*
,
سیده زینب پیغمبرزاده
2
,
مهراد بیرگانی
3
1 - 2- گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران * .
2 - 1- گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران .
3 - 1- گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران .
کلید واژه: : اشریشیا کلی, سرم آمیلوئید A , ورم پستان, استافیلوکوکوس اورئوس.,
چکیده مقاله :
این مطالعه با هدف بررسی ارزش تشخیصی سرم آمیلوئید A (SAA) در تشخیص زودهنگام ورم پستان تحت بالینی در گاوهای آلوده به اشریشیا کلی (E.coli) و عفونت های استافیلوکوکوس انجام شد. این مطالعه تحلیلی مقطعی در سال 1403 در آزمایشگاه اصفهان انجام شد و سرم آمیلوئید A را در 79 راس گاو شیری مبتلا به ورم پستان بالینی و تحت بالینی مورد ارزیابی قرار داد. گاوها به سه گروه گاوهای سالم، گاوهای مبتلا به ورم پستان تحت بالینی و گاوهای مبتلا به ورم پستان بالینی تقسیم شدند. سپس این گروه ها از نظر سرم آمیلوئید A بررسی شدند. ارزش تشخیصی سرم آمیلوئید A با محاسبه مناطق زیر منحنی (AUCs) منحنی‌های ROC تعیین شد. به طور کلی، در بین بیماران با آزمایش کشت مثبت (57٪)، 19٪ به E. coli، 22.8٪ به استرپتوکوکوس اوبریس و 15.2٪ (12 مورد) به استافیلوکوکوس اورئوس آلوده بودند. همبستگی قوی بین میانگین SCC و مقادیر SAA (P<0.005) مشاهده شد. حساسیت و ویژگی سلول های سوماتیک در شیر ((98/0) و سرم آمیلوئید A (90/0) برای تشخیص ورم پستان در گاوها بالا بود. مطالعه حاضر نشان داد که ورم پستان در گاوهای شیری با افزایش سرم آمیلوئید A است. این مطالعه نشان می دهد که تغییرات در این نشانگر زیستی می تواند برای تشخیص بیماری مورد استفاده قرار گیرد.
This study aimed to evaluate the diagnostic value of Serum Amyloid A (SAA), in the early detection of subclinical mastitis in cows infected by Escherichia coli (E.coli) and s taphylococcus aureus infections. This cross-sectional analytical study, conducted in 2024 in laboratory, Isfahan, Iran, evaluated inflammatory markers in 79 dairy cows with clinical and subclinical mastitis. The cows divided into three groups: healthy cows, cows with subclinical mastitis, and cows with clinical mastitis. These groups were then evaluated for Serum Amyloid A (SAA). The diagnostic value of the inflammatory markers was determined by calculating the areas under the curves (AUCs) of the ROC curves. Generally, among the patients with a positive culture test (57%), 19% were found to be infected with E. coli, 22.8% with Streptococcus uberis, and 15.2% (12 cases) with Staphylococcus aureus. A strong correlation was observed between the mean SCC and the values of SAA (P<0.005). The sensitivity and specificity of SCC (0.98), SAA (0.90) were high for diagnosing mastitis in cows. The present study revealed that mastitis in dairy cows is associated with an increase in inflammatory cytokines such as amyloid A. The study suggests that variations in this biomarker could be utilized for disease diagnosis
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