HPLC profile, antioxidant, antibacterial, and anti-proliferative activity of aerial parts of Ferula ovina (Boiss.) Boiss.

Subject Areas : Medicinal and Herbal Plants

Mohamad Hassan  Mohamadi ¹ (Department of Chemistry, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran)
Behnam  Mahdavi ² (Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran)
Esmaeil  Rezaei-Seresht ³ (Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran)
Sahar  Riahi-Farsani ⁴ (Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran)
Nazanin  Entezari ⁵ (Department of Anesthesiology, Faculty of Paramedical, Sabzevar University of Medical Sciences, Sabzevar, Iran)

Received: 2021-05-12 Accepted : 2021-08-28 Published : 2021-09-01

Keywords: Radical scavenging activity, Antibacterial activity, antioxidant activity, Ferrous ion chelating, Ferula ovina,

Abstract :

The aerial parts of Ferula ovina (Boiss.) Boiss. were extracted by methanol and then fractionated with solvents with different polarity. The evaluation of the bioactivity of the plant extracts including antioxidant, antimicrobial and antiproliferative activity was carried out using various assays. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. The leaves showed the highest total phenolic content and radical scavenging activity; the flowers had the maximum amount of total flavonoid content, and the stems exhibited the maximum ferrous ion chelating ability. Among ten microorganisms, the plant extract prevented the growth of three strains, namely Pseudomonas aeruginosa, Acinetobacter baumannii, and Streptococcus pneumonia. The flower of F. ovina (Boiss.) Boiss shows the best anti-human cervical carcinoma property against HeLa cell lines. Gallic acid was found as the major phenolic compound in the plant extract according to the HPLC analysis.

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