Phytoconstituents profiling of Cissus rotundifolia (Forssk.) Vahl. by HPLC-MS/MS, and evaluation of its free radical scavenging activity (DPPH) and cytotoxicity
Subject Areas : Phytochemistry: Isolation, Purification, Characterization
Ataa
Said
1
(Pharmacognosy Department, National Research Centre, Dokki, 12622 Giza, Egypt)
Elsayed Ali
Aboutabl
2
(Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-el-Aini Street, 11562 Cairo, Egypt)
Farouk Rasmy
Melek
3
(Chemistry of Natural Compounds Department, National Research Centre, Dokki, 12622 Giza, Egypt)
Gehad
Abdel Jaleel Raheem Abdel Jaleel
4
(Pharmacology Department, National Research Centre, Dokki, 12622 Giza, Egypt)
Mona
Raslan
5
(Pharmacognosy Department, National Research Centre, Dokki, 12622 Giza, Egypt)
Keywords: Cytotoxicity, HPLC-ESI-MS, Tandem mass, Free radical scavenging activity, <i>Cissus rotundifolia</i> (Forssk.) Vahl,
Abstract :
Characterization of phytoconstituents in the 70% methanolic extract of Cissus rotundifolia (Forssk.) Vahl. was performed using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Furthermore, tandem mass spectrometry (MS/MS) was performed to assist in the structural elucidation of compounds. Twenty-seven compounds were identified. The identified compounds were four acids, sixteen phenolics, two steroidal saponins, two coumarins, two stilbenoids and one triterpene. Astragalin 1, β-amyrin 2 and 1β-hydroxy-kryptogenin-1-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside 3 were isolated by column chromatography and their structures were identified by spectral analysis including 1D and 2D NMR. The plant extract and the isolated compounds 1-3 were evaluated for their radical scavenging activity. Compounds 1 and 2 showed significant activity relative to that of ascorbic acid as a standard. The cytotoxicity of the plant extract, the isolated compounds and the standard doxorubicin was evaluated using the three human tumor cell lines HT116, MCF-7 and PC-3. The plant extract exhibited significant cytotoxicity against MCF-7 while showing no activity against the other cell lines. The isolated compounds 1-3 showed no activity against the used cell lines.
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