تأثیر رزوراترول بر توانایی مهاجرت و تکثیر سلول‌های بنیادی مشتق از خون قاعدگی زنان مبتلا به اندومتریوز
محورهای موضوعی : سلولی ملکولی
هدی فضائلی
1
,
نسیم حیاتی رودباری
2
,
احسان احسانی
3
,
آذر شیخ الاسلامی
4
1 - دانشجوی دکتری، گروه زیست‌شناسی، دانشکده علوم و فناوری‌های همگرا، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - دانشیار، گروه زیست‌شناسی، دانشکده علوم و فناوری‌های همگرا، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - استادیار، گروه زیست‌شناسی، دانشکده علوم، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران
4 - استادیار، گروه سلول‌های بنیادی مزانشیمی، جهاد دانشگاهی، واحد قم، قم، ایران.
کلید واژه: اندومتریوز, رزوراترول, سلول‌های بنیادی, خون قاعدگی, زنان.,
چکیده مقاله :
هدف: 10 درصد از زنان در سنین باروری اندومتریوز را به عنوان یک بیماری التهابی و وابسته به هورمون تجربه می‌‌کنند. به دلیل نارسایی رویکردهای درمانی فعلی، استفاده از داروهای جدید و جایگزین برای بهبود درمان‌های اندومتریوز ضروری است. رزوراترول به دلیل اثرات بیولوژیکی متعددی مانند خواص آنتی نئوپلاستیک، آنتی اکسیدانی، ضد رگ‌زایی، ضد تکثیری، ضد التهابی و پیش آپوپتوزی، مورد توجه بسیاری قرار گرفته است. پروفایل بیان ژن سلول‌های بنیادی مشتق از خون قاعدگی (MenSCs) زنان اندومتریوزی و غیراندومتریوزی متفاوت است. بنابراین، در پژوهش حاضر تلاش شد که اثرات رزوراترول بر مهاجرت سلولی و میزان بیان ژنهای VEGF،KRAS و- IDO1 به عنوان عوامل مرتبط با تکثیر سلولی- درMenSCs بیماران مبتلا به اندومتریوز ارزیابی شوند.
مواد و روش‌‌ها: با استفاده از محلول فایکول، MenSCs زنان سالم (NE-MenSCs) و مبتلا به اندومتریوز (E-MenSCs) جدا شده و در آزمایشگاه کشت داده شدند. E-MenSCs با 100 µM رزوراترول به مدت 72 ساعت تیمار شدند، در حالی‌که در گروه‌های NE-MenSCs و E-MenSCs، سلول‌ها طی این مدت هیچ درمانی دریافت نکردند. بررسی توانایی مهاجرت و سطح بیان ژن‌‌ها، با استفاده از آزمون خراش وReal-time PCR انجام شد.
یافته‌ها: پس از 36 ساعت، در هر دو گروه NE-MenSCs و Resveratrol درصد بسته شدن خراش به طور معنی‌داری کمتر از گروه E-MenSCs بود. همچنین بیان ژنهای KRAS و VEGF در گروه Resveratrol به‌طور معنی‌داری کمتر از گروه E-MenSCs بود.
نتیجه‌‌گیری: رزوراترول می‌تواند پیشرفت اندومتریوز را از طریق کاهش توانایی مهاجرت سلول‌ها و کاهش بیان VEGF و KRAS کاهش دهد.
Objective: 10% of women of reproductive age experience endometriosis as an inflammatory and hormone-dependent disease. Due to the failure of current treatment approaches, it is necessary to use new and alternative drugs to improve endometriosis treatments. Resveratrol has received much attention due to its many biological effects such as antineoplastic, antioxidant, anti-angiogenic, anti-proliferative, anti-inflammatory and pro-apoptotic properties. The gene expression profile of menstrual blood-derived stem cells (MenSCs) of endometriotic and non-endometriotic women is different. Therefore, in the present study, an attempt was made to evaluate the effects of resveratrol on cell migration and the expression levels of VEGF, KRAS, and IDO1 genes as factors related to cell proliferation in MenSCs of patients with endometriosis.
Materials and methods: MenSCs from healthy women (NE-MenSCs) and endometriosis (E-MenSCs) were isolated and cultured in the laboratory using Faycol solution. E-MenSCs were treated with 100 µM resveratrol for 72 hours, while in
NE-MenSCs and E-MenSCs groups, the cells did not receive any treatment during this period. Investigating the ability of migration and expression level of genes was done using scratch test and real-time PCR.
Findings: After 36 hours, in both NE-MenSCs and Resveratrol groups, the scratch closure percentage was significantly lower than the E-MenSCs group. Also, the expression of KRAS and VEGF genes in the Resveratrol group was significantly lower than the E-MenSCs group.
Conclusion: Resveratrol can reduce the progression of endometriosis by reducing the ability of cells to migrate and reducing the expression of VEGF and KRAS.
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