اثرپیش تیمار با غلظت‌های مختلف اوره بروی خصوصیات فیزیولوژیکی و بیوشیمایی ذرت (Zea mays L.) تحت تنش شوری
محورهای موضوعی : تکنولوژی بذر
هما زارعی
1
,
محمد صدقی
2
,
سلیم فرزانه
3
,
هانیه سعادت
4
1 - دانشجوی کارشناسی ارشد علوم و تکنولوژی بذر، دانشگاه محقق اردبیلی دانشکده کشاورزی و منابع طبیعی، اردبیل، ایران،
2 - استاد گروه علوم و مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.
3 - استادیار گروه زراعت و اصلاح نباتات دانشگاه محقق اردبیلی
4 - دکتری اکولوژی، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه زراعت و اصلاح نباتات
کلید واژه: آنزیم‌های آنتی‌اکسیدانت, اوره, پرایمینگ, کلرید سدیم,
چکیده مقاله :
به منظور بررسی اثر پیش‌تیمار با غلظت‌های مختلف اوره روی خصوصیات فیزیولوژیکی و بیوشیمیایی ذرت تحت تنش شوری، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملا تصادفی در سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا گردید. فاکتورهای مورد بررسی سطوح مختلف شوری (صفر، 100 و ۲۰۰ میلی‌مولار) و سطوح مختلف محلول اوره (صفر، 5/1 و ۳ درصد) بود. نتایج نشان داد شوری درصدجوانه‌زنی، سرعت جوانه‌زنی، یکنواختی جوانه‌زنی، طول ریشه‌چه و ساقه‌چه، وزن تر و خشک ریشه‌چه و ساقه‌چه را کاهش داد. ولی پیش‌تیمار با اوره این صفات را بهبود بخشید. بیش‌ترین میانگین مدت جوانه‌زنی در شوری200 میلی‌مولار و شاهد (آب مقطر) مشاهده شد. فعالیت آنزیم‌های کاتالاز و پراکسیداز با تشدید شوری افزایش یافتند و بیش‌ترین مقدار آن‌ها در شوری 200 میلی‌مولار مشاهده شد. پیش‌تیمار با محلول اوره 3 درصد این آنزیم‌ها را بهبود بخشید. فعالیت آنزیم سوپراکسید دیسمیوتاز در پرایمینگ با اوره 3 درصد و شوری200 میلی‌مولار نسبت به شاهد حدود 61 درصد افزایش نشان داد. آنزیم آمیلاز و پروتئین در پیش‌تیمار با اوره 3 درصد و بدون شوری نسبت به شاهد به ترتیب حدود 73 و 70 درصد افزایش نشان دادند. با توجه به نتایج مشاهده شده بذرهای پرایم شده با محلول اوره 3 درصد بیش‌ترین تاثیر را در شرایط تنش شوری روی ذرت داشتند.
In order to investigate Effect of different concentrations of urea on physiological and biochemical properties of maize (Zea mays L.) under Salinity Stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2021. The investigated factors were different levels of salinity (zero, 100 and 200 mM) and different levels of urea solution (zero, 1.5 and 3%). The results showed that salinity stress decreased Germination Percentage (GP), Germination Rate (GR), Germination uniformity (GU), Radicle and Pedicel Length (RL and PL) and Radicle Fresh and Dry Weight (RFW and RDW), But priming with urea improved these traits. The highest Medium Germination Time (MGT) was related at 200 mM salinity and control (distilled water). The activity of catalase and peroxidase enzymes increased with salinity intensification and the highest amount was observed at 200 mM salinity. Priming with 3% urea solution improved these enzymes. The superoxide dismutase enzyme activity in priming with 3% urea and 200 mM salinity compared to the control showed an increase about 61%. Amylase and protein in pretreatment with urea 3% and without salinity compared to the control showed an increase respectively about 73% and 70%. According to the observed results, seeds primed with 3% urea solution had the greatest effect on salinity stress in maize. According to the observed results, seeds primed with 3% urea solution had the greatest effect on salinity stress in maize.
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