مطالعهی تاثیر برخی متابولیتهای تولید شده توسط Pseudomonas tolaasii و باکتریهای همراه روی قارچ خوراکی (Agaricus bisporus)
محورهای موضوعی :
دو فصلنامه تحقیقات بیماریهای گیاهی
شادی تجلی پور
1
,
نادر حسن زاده
2
,
اصغر حیدری
3
,
حسین خباز جلفایی
4
1 - دانش آموخته کارشناسی ارشد، گروه بیماری شناسی گیاهی، دانشگاه آزاد اسلامی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات تهران، تهران، ایران.
2 - دانشیار، گروه بیماری شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران.
3 - دانشیار، گروه بیماری شناسی گیاهی، موسسه تحقیقات گیاهپزشکی کشور، تهران، ایران.
4 - مربی، گروه بیماری شناسی گیاهی، موسسه تحقیقات گیاهپزشکی کشور، تهران، ایران.
تاریخ دریافت : 1394/11/12
تاریخ پذیرش : 1394/11/12
تاریخ انتشار : 1395/03/01
کلید واژه:
قارچ دکمهای سفید,
لکه قهوهای,
Agaricus bisporus,
Pseudomonas reactans,
Pseudomonas tolaasii,
چکیده مقاله :
دراین تحقیق به بررسی برخی متابولیتهای Pseudomonas tolaasiiعامل لکه قهوهای قارچ خوراکی دکمهای و نیز Pseudomonas reactansباکتری همراه قارچ خوراکی دکمهای، پرداخته شد. خصوصیات شاخصP. Tolaasiiشامل ایجاد لکه قهوهای و نقاط فرو رفته روی کلاهک قارچ خوراکی دکمهای A. bisporus، تولید تولاسین، همولیزاریتروسیتها، تولید رسوب سفید رنگ در برابر باکتری P. reactans است. در مطالعهی حاضر تأثیرP. Tolaasiiو متابولیت حاصل از آن بر میزان رشد میسلیوم Agaricus bisporus مورد بررسی قرار گرفت. پس از استخراج ترکیبات ناخالص متابولیتی یاWhite Line Inducing Principle (WLIP)، تولید شده توسط P. reactansو نیز توکسین تولاسین، آزمونهای متعددی جهت بررسی تأثیر متقابل و غیرمستقیم این ترکیبات انجام گرفت. همچنین ژن rpoBمتعلق به جدایه بیماریزای Pseudomonas sp. p2 که از قارچ مذکور جداسازی شده بود با استفاده از جفت پرایمر LAPS27 و LAPS تکثیر، تعیین توالی و در بانک ژن NCBI ثبت گردید. تأثیر ترکیب WLIP ناخالص حاصل از P. reactans Pr 5 و جدایه استاندارد P. reactans NCPPB1311 بر بلوکهای A. bisporus وسیبزمینی بطور جداگانه مورد بررسی قرار گرفت. در زیست سنجیهای مختلف مشاهده شد بلوکهای قارچ مایهزنی شده با توکسین بیماریزاترین استرین بومی P. tolaasii Pt 6 تولید علایم لکه قهوهای تیره و نقاط کمی فرو رفته نمودند. برعکس، در برابرترکیب WLIP استرین P. reactans Pr 5 تغییر رنگ جزئی و خفیف بود. از یافتههای مهم دیگر، بکارگیری همزمان تیمار P. reactans A6 و ترکیب تولاسین هر دو جدایۀ بومی و استاندارد بر بلوک قارچ بود که منجر به توقف علایم آشکار قهوهای شدن ناشی از تولاسین شد و با خاصیت بازدارندگی خود، موجب مهار فعالیت تولاسین گردید. این نتایج نشاندهنده اهمیت باکتریهای همراه در بازداری رشد و توقف بیماریزائی باکتری P. tolaasiiاست
.
چکیده انگلیسی:
In this research some metabolites of Pseudomonas tolaasii the causal agent of mushroom bacterial blotch disease and Pseudomonas reactans associated with it were investigated. Characteristic features of P. tolaasii included brown pit formation on button mushroom caps, tolaasin production, lysis of erythrocytes, and white line induction in agar plate in presence of Pseudomonas reactans. Further, the metabolites of P. tolaasii and mushroom-associated bacteria were extracted and their effects were evaluated against the blocks of the mushroom A. bisporus. It was noticed that the strains of P. reactans also produced a lipodepsipeptide, WLIP. Certain complementary tests were also conducted to confirm the presence of these compounds in products extracted. The rpoB gene of a pathogenic strain of Pseudomonas sp. p2 isolated from the same mushroom was amplified using the primer pair of LAPS and LAPS27 and sequenced and deposited in Genebank at NCBI. Effect of impure WLIP of the strain P. reactans Pr 5 and standard strain of P. reactans NCPPB1311 were examined on blocks of A. bisporus and potato tubers. In bioassays, the tolaasin of strain P. tolaasii Pt 6 created both brown blotch and slight pitting symptoms. Whereas, WLIP of the P. reactans Pr 5 could only induce slight discoloration. Another important finding was that simultaneous application of P. reactans A6 in combination with tolaasins of P. tolaasii Pt 6 or P. tolaasii NCPPB2192 resulted in suppression of brown blotch symptoms on treated blocks with P. tolaasii. Results indicate the importance of associated bacteria in suppression of disease by P. tolaasii.
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