بررسی فاکتورهای بیماریزایی سفیدک پودری جو در استان فارس
محورهای موضوعی :
دو فصلنامه تحقیقات بیماریهای گیاهی
محمد جواد مرمت
1
,
سیدمحمدرضا موسوی
2
,
منصوره کشاورزی
3
,
محمد تائب
4
1 - کارشناس ارشد دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران.
2 - دانشگاه آزاد اسلامی واحد مرودشت، گروه بیماری شناسی گیاهی، مرودشت، ایران.
3 - موسسه تحقیقات اصلاح نهال و بذر، کرج، ایران.
4 - دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، ایران.
تاریخ دریافت : 1392/08/13
تاریخ پذیرش : 1392/08/13
تاریخ انتشار : 1392/07/01
کلید واژه:
جو,
سفیدک پودری,
لاین های تقریباً ایزوژنیک,
فاکتورهای بیمار یزایی,
چکیده مقاله :
اولین گام در جهت تشخیص و تهیه ارقام مقاوم به بیماری سفیدک پودری جو، شناسایی فاکتورهای بیماریزایی جمعیت عامل آن است. بدین منظور در تحقیقی که در سال 1387 در استان فارس صورت گرفت تعداد ده جدایه از مناطق مختلف استان جمع آوری و روی یک رقم حساس جو تکثیر شدند. سپس جداگانه روی گیاهچه تعداد 19 رقم تقریباً ایزوژنیک که رقم پایه آنها پالاس و دارای ژنهای مقاومت متفاوتی بودند و یک رقم حساس مایه زنی شدند. واکنش لاین ها از نظر دوره کمون، تیپ و شدت آلودگی یادداشت برداری شد. نتایج نشان داد که فاکتورهای بیماریزایی در استان فارس فراوانی متغیری دارند . بیماریزایی برای ژن های مقاومتMl-a 3 و Ml-a23 دارای بیشترین فراوانی در مناطق مورد اجرای آزمایش بوده وپس از آن بیشترین فراوانی بیماری زایی به ترتیب برای ژ ن های، Ml-K ،Ml-a7+Ml(No3)،Ml-hوMl-a12+Ml(Em2) ثبت گردید. کمترین فراوانی بیماری زایی برای ژن های مقاومتMl-a 22 وMl-a6+Ml- a14 دیده شد برای ژنهای Ml-(La)،Ml-a13+Ml-(Ru3) ،Ml-a10+Ml-(DU2) ،Ml-a9 ،Ml-al+Ml(a12) ،Ml-a8 ،Ml-05 ،Ml--Ml-(cp) در هیچ یک از مناطق بیماری زایی دیده نشد Ml-at و Ml-p ،Ml- K(1). دوره کمون تمام جدایه ها تقریباً از یک روند زمانی خاص پیروی می کرد.
چکیده انگلیسی:
Determining the pathogenicity factors of barely powdery mildew casual agent is the first step in identifying and producing resistant cultivars. During growing season 2008, ten isolates of barely powdery mildew were collected from different parts of Fars province (Iran). Mass propagated on a susceptible barely cultivar. Seedlings of 19 near-isogenic barley lines, obtained from Palas base type and containing various resistance genes, were inoculated with the collected isolates. A susceptible cultivar (cv. Afzal) was used as positive control. Later on,reactions of lines were recorded according to latent period, type and severity of disease. The detected pathogenicity factors in Fars province seemed to have variable frequencies.Virulence factors against Ml-a3 and Ml-a23 resistance genes were the most abundant in the tested area, while for Ml-K, Ml-a7+Ml-(NO3), Ml-h and Ml-a12+Ml (Em2) genes were the second most abundant. For the Ml-a22 and Ml-a6+Ml-a14 the least frequency of pathogenic potential was observed. There are no pathogenicity factors against Ml-05, Ml-g-Ml-(Cp), Ml-(La),Ml-a8, (Ml-a1, Ml(A12)), Ml-a9, Ml-a10+Ml-(DU2), Ml-a13+Ml-(Ru3), Ml-K(1), Ml-P and Ml-at resistance genes in Fars province. Almost all barley lines followed a similar pattern in their latent period.
منابع و مأخذ:
Agrios, G.N. 2005. Plant Pathology. 5th edition. Academic Press, San Diego, USA.
Alexopoulos, C.J., Mims, C.W. and Blackwell, M. 1996. Introductory Mycology. 4th edition. John Wiley & Sons Inc., New York, USA.
Behdad, E. 2006. Phytopathology and Important Plant Diseases in Iran. Atre-Etrat Pub., Ghom, Iran (in Persian).
Behnya, M.R. 1994. Cold Region's Cereals. University of Tehran Pub. Tehran. 473 pp.
Browning, J.A. and Frey, K.J. 1969. Multiline cultivars as a means of disease control. Annual Review of Phytopathology 7: 355–382.
Chaube, H.S. and Pundhir, V.S. 2005. Crop Disease and Their management. Prentice Hell, New Delhi, India.
Czembor, J.H., and Czembor, H.J. 1998. Powdery mildew resistance in cultivars of spring barley from polish register. Plant Breeding and Seed Science 42: 87–99.
Czembor, J.H., and Czembor, H.J. 1999.Powdery mildew resistance in cultivars of winter barley form polish register. Plant Breeding and Seed Science 43: 65–75.
Czembor, J.H., and Czembor, H.J. 2000. Powdery mildew resistance in selection from Moroccan barley landraces. Phytoparasitica 28: 65–78.
Hossain, M.A. and Rahman, M.S. 1993. Pathogenic variability of Erysiphe graminis f.sp. hordei in south Australia, 1981-1985 . Australian Journal of Agricultural Research 44: 1931–1945.
Hovmoller, M.S., Caffier, V., Jalli, M., Anderson, O., Besenhofer, G., Czembor, J.H., Dreiseitl, A., Flath, K., Fleck, A., Heinrics, F., Jonsson, R., Limpert, E., Mercer, P., Plesnik, S., Rashal, I., Skinnes, H., Slater, S. and Vronska, O. 2000. The European barley powdery mildew virulence survey and disease nursery 1993-1999. Agronomia 20: 729–744.
Hovmoller, M.S., Munk, L. and Ostergard, H. 1993. Observed and predicted changes in virulence gene frequencies at 11 loci in a local barley powdery mildew population. Phytopathology 83: 253–260.
Jorgensen, J.H. 1994. Genetics of powdery mildew resistance in barley. Critical Review in Plant Science 13: 97–119.
Khodabandeh, N. 1998. Cereals. University of Tehran Publishing, Tehran, Iran (in Persian).
Limpert, E. 1987. Spread of barley mildew by wind, its significance for phytopathology. Advancements in Aerology 33: 331–336.
Limpert, E., Andrivon, D. and Fischbeck, G. 1990. Virulence pattern in populations of Erysiphe graminis f.sp. hordei in 1986. Plant Pathology 39: 402-415.
Limpert, E., Godet, F. and Muller, K. 1999. Dispersal of cereal mildews across Europe. Agricultural and Forest Meteorology 97: 293–308.
Limpert, E., Muller, K., Duan, X., Koller, B., Mcdermott, J. and Wolfe, M.S. 1991. Barley mildew in Europe-Towards an integrated analysis of the pathogen, including virulence, fungicide sensitivity and RFLPs. pp. 213-221. In: Jorgenson, J.H. (ed). Integrated Control of Cereal Mildews, Virulence Patterns and Their Changes. Riso National Laboratory, Roskilde, Denmark.
Mains, E.S. and Dietz, S.M. 1930. Physiological forms of barley mildew, Erysiphe graminis f.sp. hordei Marchal. Phytopathology 20: 229–239.
Marshall, D.R. 1977. The advantages and hazards of genetic homogenicity. Annals of New York Academic Science 278: 1–20.
Munck, L. 1981. Barley for food, feed and industry. pp. 427–459. In Pomeranz Y and Munck L. (eds). Cereals: A Renewable Resource. The American Association of Cereal Chemists, St. Paul, MN, USA.
Nour Mohamadi, GH., Siadat, A. and Kashani, A. 2001. Agronomy (Cereals). University of shahid-Chamran Publishing, Ahwaz, Iran.
Patpour, M., Torabi, M., Aghnom, R., Dehghan, M.A., Dad-Rezaei, T., Afshari, F. and Ahmadian-Moghadam, M.S. 2005. Virulence factors of barley powdery mildew pathogen and their variation in some parts of Iran during 2000-2002. Seed and Plant 2: 303–313 (in Persian).
Poehlman, J.M. 1983. Breeding Field Crop. 2nd edition.the AVI Publishing, Company Inc., Westport, CT, USA.
Pourmansouri, T. 1998. Virulence factors of Erisiphe graminis f.sp. hordei in samples collected from different part of Iran. 13th Iranian Plant Protection Congress. Karaj, Iran.
Schonfeld, M., Fischbeck, G. and Jahoor, A. 1994. Identifizierung und lokalisierung der mehltauresistenz gene aus der wildgerste und deren moglicher einsatz in der resistenzzuchtung. Vortrage Fur PflLanzenzuchtung 28: 184–186.
Spencer, D.M. 1978. The Powdery Mildews. Academic Press, London, UK.
Stoelen, O., Hermansen, J.E. and Lohde, J. 1980. Varietal mixtures of barley and their ability to reduce powdery mildew and yellow rust disease. Kongelige Veterinaer- og Landbohoejskole Aarsskrift 1980: 109–116.
Taherei, F., Keshavarzi, M., Patpoor, M., Valad-Abadi S.A.R. and Soltanloo, H. 2007. Genetic analysis of resistance to powdery mildew in barley. Seed and Plant 23: 311–323 (In Persian).
Tinker, N.A. and Mather, D.E. 1994. Main effects of quantitative trait loci in Harrington /TR306 two-row barely. Barley Genetic Newsletter 23: 72–78.
Wei, F., Gobelman-Werner, Morroll, S.M., Kurth, J., Mao, L., Wing, R., Leister, D., Schulze-Lefert, P. and Wise, R.P. 1999. The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and supported recombination within a 240-kb DNA internal on chromosome 55 (1HS) of barley. Genetics 153: 1929–1948.
Wolfe, M.S. and Limpert, E. 1987. Integrated Control of Cereal Mildews: Monitoring the Pathogen. Martinus Nijhoff Publishers, Dordrecht, the Netherlands.
Yahyaoui A.H., Reinhold, M. and Scharen, A.L. 1997. Virulence spectrum in populations of barley powdery mildew pathogen, Erysiphe graminis f. sp. hordei in Tunisia and Morocco in 1992. Plant Pathol. 46: 139–146.
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