اثر تمرین تناوبی بر بیان عامل رونویسی میتوکندری (TFAM) در بافت عضله دوقلوی موش-های نر ویستار تحت عصاره دود سیگار
محورهای موضوعی : نشانگر های زیستی پلاسما
مهدی بخشی
1
,
عبدالعلی بنائی فر
2
*
,
سجاد ارشدی
3
,
بهزاد بازگیر
4
1 - دانشجوی دکتری فیزیولوژی ورزش دانشکده تربیت بدنی دانشگاه آزاد اسلامی واحد تهران جنوب، تهران، ایران.
2 - دانشگاه ازاد اسلامی واحدتهران جنوب
3 - Assistant Professor of Exercise Physiology , South Tehran Branch , Islamic Azad university, Tehran, Iran.
4 - baqiyatallah university of medical sciences
کلید واژه: تمرین تناوبی , بافت عضله دو قلو, عصاره دود سیگار, بیان ژن, TFAM,
چکیده مقاله :
زمینه و هدف: مطالعات نشان می دهد قرار گرفتن در معرض دود سیگار می تواند با افزایش پروتئولیز بافت عضلانی سنتز پروتئین را مهار نموده و باعث اختلال در عوامل موثر بر عملکرد میتوکندری گردد. هدف از پژوهش حاضر مطالعه تاثیر تمرینات تناوبی بر بیان TFAM در موش‌های صحرایی نر ویستار تحت عصاره دود سیگار میباشد.
مواد و روشها :
در این پژوهش، 28 سر موش صحرایی نر نژاد ویستار (با سن 8 هفته) با میانگین وزنی 80/10 ± 12/220 گرم انتخاب و به چهار گروه کنترل ، عصاره دود سیگار، تمرین تمرین + عصاره دود سیگار تقسیم شدند. عصاره دود سیگار(CSE) حاوی 12 میلی گرم قطران و 0.9میلی گرم نیکوتین بود. به گروه کنترل سالین و گروه CSE عصاره دود سیگار یک روز در هفته برای 6 هفته تزریق گردید. پروتکل تمرینات تناوبی هر جلسه 49 دقیقه، بصورت 5 بار در هفته و به مدت 6 هفته انجام شد. پس از آخرین جلسه تمرین، موشها بیهوش و بافت عضلانی آنها جدا و به آزمایشگاه منتقل گردید. سپس بیان ژن مورد نظر اندازه گیری شد. تجزیه و تحلیل یافته‌های با آزمون ANOVA و از آزمون تعقیبی LSD استفاده شد (05/0≥p).
نتایج:
نتایج نشان داد بیان TFAM درگروه عصاره دود سیگار کاهش معنی داری نسبت به گروه کنترل سالم نشان داد (046/0P=). اما در گروه تمرین و گروه تمرین + عصاره دود سیگار نسبت به دیگر گروهها تفاوت معنی داری مشاهده نشد (05/0P≥).
نتیجه گیری : نتایج نشان داد، عصاره دود سیگار سبب کاهش معنی دار بیان TFAM در عضله دو قلوگردید. همچنین به نظر می رسد تمرین تناوبی در کنار دود سیگار، با افزایش عملکرد TFAM می تواند اثرات استرس اکسیداتیو ناشی از عصاره دود سیگار را کاهش دهد و از mtDNA در برابر تخریب ROS محافظت نموده و به بهبود بیوژنز میتوکندری کمک نماید.
Background and aim: Studies show that exposure to cigarette smoke can inhibit protein synthesis by increasing proteolysis of muscle tissue and disrupt factors affecting mitochondrial function. The aim of the present study was to study the effect of interval training on TFAM expression in male Wistar rats exposed to cigarette smoke extract.
Materials and Methods:
In this study, 28 male Wistar rats (8 weeks old) with an average weight of 220.12 ± 10.80 g were selected and divided into four groups: control, cigarette smoke extract, exercise, and exercise + cigarette smoke extract. Cigarette smoke extract (CSE) contained 12 mg of tar and 0.9 mg of nicotine. The saline control group and the CSE group were injected with cigarette smoke extract one day a week for 6 weeks. The interval training protocol was performed for 49 minutes per session, 5 times a week for 6 weeks. After the last training session, the rats were anesthetized and their muscle tissue was isolated and transported to the laboratory. Then, the expression of the target gene was measured. The findings were analyzed with ANOVA and the LSD post hoc test was used (p≥0.05).
Results:
The results showed that TFAM expression in the cigarette smoke extract group showed a significant decrease compared to the healthy control group (P=0.046). However, there was no significant difference in the exercise group and the exercise + cigarette smoke extract group compared to the other groups (P≥0.05).
Conclusion: The results showed that cigarette smoke extract caused a significant decrease in TFAM expression in the gastrocnemius muscle. It also seems that intermittent training with cigarette smoke, by increasing TFAM function, can reduce the effects of oxidative stress caused by cigarette smoke extract and protect mtDNA against ROS damage and help improve mitochondrial biogenesis.
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