تأثیر تمرینات تداومی و تناوبی بر بیوژنز میتوکندری بافت هیپوکامپ رت های دیابتی القاء شده با STZ
محورهای موضوعی : فعالیت بدنی و تندرستی
نیما نجمی
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پروین فرزانگی
2
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لیدا مرادی
3
1 - گروه تربیت بدنی و علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.
3 - گروه تربیت بدنی و علوم ورزشی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران.
کلید واژه: شدت تمرین, بیوژنز میتوکندریایی, بیماری متابولیک, هیپوکامپ.,
چکیده مقاله :
هدف: هدف از تحقیق حاضر بررسی تاثیر تمرین ورزشی تداومی و تناوبی بر بیان ژن PGC-1α و TFAM بافت هیپوکمپ در رتهای دیابتی القاء شده با STZ بود.
روش : در این مطالعه تجربی، تعداد 20 موش نر نژاد ویستار به طور تصادفی به دو گروه کنترل (5=n) و دیابت (15=n) تقسیم شدند. القاء دیابت در موش های گروه بیمار توسط تجویز Streptozotocin با غلظت mg/kg 50 صورت گرفت. موش های دیابتی به 3 گروه (1) کنترل دیابتی (2) تمرین تداومی- دیابت (3) تمرین تناوبی- دیابت تقسیم شدند.گروه‌های تمرین تداومی و تناوبی به مدت هشت هفته و هفته ای 5 جلسه تمرینات موردنظر را انجام دادند. سطوح بیان ژن I PGC1-α و TFAM با استفاده از روش Real Time PCR انحام شد. تجزیه و تحلیل داده ها با استفاده از آزمون آنالیز واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنی‌داری p<0.05 انجام شد.
یافته ها: یافته های تحقیق نشان داد که سطوح بیان ژن PGC1-α و TFAM در موش های دیابتی در مقایسه با گروه کنترل سالم کاهش معناداری داشت. تمرینات تداومی و تناوبی موجب افزایش در سطوح بیان ژن PGC1-α و TFAM در گروه های تمرین در مقایسه با گروه کنترل بیمار شدند که این روند تغییرات در گروه تمرین تناوبی به سطح معناداری رسید.
نتیجه‌گیری: نتایج حاکی از آن است که تمرینات ورزشی، به‌ویژه تمرینات تناوبی شدید، می‌توانند راهکاری مؤثر برای تحریک بیوژنز میتوکندریایی و بهبود شاخص‌های متابولیکی در شرایط پاتولوژیک باشند.
Aim:
This study aimed to investigate the impact of continuous and interval exercise training on the expression of PGC-1α and TFAM genes in the hippocampal tissue of rats with streptozotocin (STZ)-induced diabetes.
Method:
In this experimental study, twenty male Wistar rats were obtained from the Pasteur Institute's animal facility. After a one-week acclimation period, the rats were randomly assigned into two initial groups: healthy controls and diabetic. Diabetes was induced via administration of STZ at a dosage of 50 mg/kg. Diabetic rats were subsequently allocated into three subgroups: (1) diabetic control, (2) diabetic with continuous exercise, and (3) diabetic with interval exercise. Exercise protocols were conducted over eight weeks, five sessions per week, incorporating aerobic and resistance modalities. Gene expression levels of PGC-1α and TFAM in hippocampal tissue were measured using Real-Time PCR. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test at a significance level of p<0.05.
Results:
Gene expression levels of PGC-1α and TFAM were significantly reduced in diabetic rats compared to healthy controls. Both continuous and interval exercise training led to increased expression of these genes in the trained diabetic groups relative to the diabetic control group, with interval training resulting in a statistically significant improvement.
Conclusion:
These results indicate that structured physical activity, particularly high-intensity interval training, may serve as an effective strategy to stimulate mitochondrial biogenesis and improve metabolic parameters under pathological conditions such as diabetes
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