The Effect of Continuous and Interval Training on Mitochondrial Biogenesis in the Hippocampal Tissue of Streptozotocin-Induced Diabetic Rats
Subject Areas : Physical Activity and Health
nima najmi
1
,
پروین فرزانگی
2
*
,
lida moradi
3
1 - Department of physical Education and Sports Science, NT.C., Islamic Azad University, tehran, Iran.
2 - Department of Physical Education and Sports Science, Sa.C., Islamic Azad University, Sari, Iran.
3 - Department of physical Education and Sports Science, NT.C., Islamic Azad University, tehran, Iran.
Keywords: Training Intensity, Mitochondrial Biogenesis, Metabolic Disease, Hippocampus.,
Abstract :
Aim:
This study aimed to investigate the impact of continuous and interval exercise training on the expression of PGC-1α and TFAM genes in the hippocampal tissue of rats with streptozotocin (STZ)-induced diabetes.
Method:
In this experimental study, twenty male Wistar rats were obtained from the Pasteur Institute's animal facility. After a one-week acclimation period, the rats were randomly assigned into two initial groups: healthy controls and diabetic. Diabetes was induced via administration of STZ at a dosage of 50 mg/kg. Diabetic rats were subsequently allocated into three subgroups: (1) diabetic control, (2) diabetic with continuous exercise, and (3) diabetic with interval exercise. Exercise protocols were conducted over eight weeks, five sessions per week, incorporating aerobic and resistance modalities. Gene expression levels of PGC-1α and TFAM in hippocampal tissue were measured using Real-Time PCR. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test at a significance level of p<0.05.
Results:
Gene expression levels of PGC-1α and TFAM were significantly reduced in diabetic rats compared to healthy controls. Both continuous and interval exercise training led to increased expression of these genes in the trained diabetic groups relative to the diabetic control group, with interval training resulting in a statistically significant improvement.
Conclusion:
These results indicate that structured physical activity, particularly high-intensity interval training, may serve as an effective strategy to stimulate mitochondrial biogenesis and improve metabolic parameters under pathological conditions such as diabetes
1. Tao LC, Wang TT, Zheng L, Hua F, Li JJ. The Role of Mitochondrial Biogenesis Dysfunction in Diabetic Cardiomyopathy. Biomol Ther (Seoul). 2022;30(5):399-408. doi:10.4062/biomolther.2021.192
2. Xue H, Li P, Luo Y, et al. Salidroside stimulates the Sirt1/PGC-1α axis and ameliorates diabetic nephropathy in mice. Phytomedicine. 2019;54:240-247. doi:10.1016/j.phymed.2018.10.031
3. Choi J, Chandrasekaran K, Inoue T, Muragundla A, Russell JW. PGC-1α regulation of mitochondrial degeneration in experimental diabetic neuropathy. Neurobiol Dis. 2014;64:118-130. doi:10.1016/j.nbd.2014.01.001
4. She Y, Yu M, Wang L, Wang Y, Fang P, Zhang Z. Emerging Protective Actions of PGC-1α in Diabetic Nephropathy. Oxid Med Cell Longev. 2022 Oct 10;2022:6580195. doi: 10.1155/2022/6580195.
5. Yuan CX, Wang X, Yu Z, Lian XY, Xu B. Electroacupuncture improves peripheral neuropathy by up-regulating Sirt1/PGC-1α/TFAM pathway in type 2 diabetes rats with peripheral neuropathy. Zhen ci yan jiu= Acupuncture research. 2024 Apr 1;49(4):349-57. doi: 10.13702/j.1000-0607.20221318.
6. Zhang Q, Song W, Zhao B, Xie J, Sun Q, Shi X, Yan B, Tian G, Liang X. Quercetin attenuates diabetic peripheral neuropathy by correcting mitochondrial abnormality via activation of AMPK/PGC-1α pathway in vivo and in vitro. Frontiers in Neuroscience. 2021 Mar 3;15:636172.. doi: 10.3389/fnins.2021.636172.
7. Yerra VG, Kumar A. Adenosine monophosphate-activated protein kinase abates hyperglycaemia-induced neuronal injury in experimental models of diabetic neuropathy: effects on mitochondrial biogenesis, autophagy and neuroinflammation. Molecular neurobiology. 2017 Apr;54(3):2301-12. doi: 10.1007/s12035-016-9824-3
8. Sagarkar S, Balasubramanian N, Mishra S, Choudhary AG, Kokare DM, Sakharkar AJ. Repeated mild traumatic brain injury causes persistent changes in histone deacetylase function in hippocampus: Implications in learning and memory deficits in rats. Brain research. 2019 May 15;1711:183-92. doi: 10.1016/j.brainres.2019.01.022. Epub 2019 Jan 18. PMID: 30664848.
9. Balasubramanian N, Jadhav G, Sakharkar AJ. Repeated mild traumatic brain injuries perturb the mitochondrial biogenesis via DNA methylation in the hippocampus of rat. Mitochondrion. 2021 Nov 1;61:11-24. doi: 10.1016/j.mito.2021.09.001.
10. Zhang Q, Liang XC. Effects of mitochondrial dysfunction via AMPK/PGC-1 α signal pathway on pathogenic mechanism of diabetic peripheral neuropathy and the protective effects of Chinese medicine. Chinese Journal of Integrative Medicine. 2019 May;25(5):386-94. doi.org/10.1007/s11655-018-2579-0
11. Gureev AP, Shaforostova EA, Popov VN. Regulation of mitochondrial biogenesis as a way for active longevity: interaction between the Nrf2 and PGC-1α signaling pathways. Frontiers in genetics. 2019 May 14;10:435.. doi: 10.3389/fgene.2019.00435.
12. Shirvani H, Arabzadeh E. Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α. Eating and Weight Disorders-Studies on Anorexia, Bulimia and Obesity. 2020 Feb;25(1):17-24. doi: 10.1007/s40519-018-0491-4.
13. Shirvani H, Arabzadeh E. Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α. Eating and Weight Disorders-Studies on Anorexia, Bulimia and Obesity. 2020 Feb;25(1):17-24. doi: 10.1007/s40519-018-0491-4.
14. Peng C yan, Xie Q yuan, Xie X, Tang L yi, Ma T xin, Ke D wei, et al. Extraction, phytochemicals characterization, in vivo and in vitro anti-diabetic ability of non-extractable polyphenols from Undaria pinnatifida. Food Research International [Internet]. 2024 Nov;196:115021. Available from: http://dx.doi.org/10.1016/j.foodres.2024.115021
15. Burke JA, Chen AR, Chansky J. Long-term storage, cryopreservation, and culture of isolated human islets: a systematic review. Frontiers in Transplantation.;4:1614849.
16. Mohsenzadeh M, Aghaei F, Nameni F. The effect of high intensity interval training on SIRT1 and PGC1-α gene expression in soleus muscle of type 2 diabetes in male rat. Medical Journal of Tabriz University of Medical Sciences. 2020 Oct 28;42(4):447-55. [In Persian]
17. Ebrahimnezhad N, Nayebifar S, Soltani Z, Khoramipour K. High-intensity interval training reduced oxidative stress and apoptosis in the hippocampus of male rats with type 2 diabetes: The role of the PGC1α-Keap1-Nrf2 signaling pathway. Iranian Journal of Basic Medical Sciences. 2023;26(11):1313. [In Persian]
18. Ahmadi F, Siahkouhian M, Mirdar S, Tapak L. The Effect of a Detraining After Resistance Training on the Histochemical Expression of Potassium Channels and Mitochondrial Biogenesis of Heart Tissue in Male Rats. Internal Medicine Today. 2021 Mar 10;27(2):230-45. [In Persian]
19. Baghadam M, Mohammadzadeh Salamat K, Azizbeigi K, Baesi K. The effect of resistance training on IRISIN and gene expression of PGC1α in the cardiac muscle in STZ-Induced diabetic rats. Community Health Journal. 2018 Sep 23;12(3):58-64. [In Persian]
20. Bakhtiyari A, GAEINI A, Choobineh S, KORDI M, Hedayati M. The comparison of the influence of 12-week high-intensity interval training and continuous moderate intensity training on PGC-1α and Tfam mitochondrial proteins expressions in gastrocnemius muscle of elderly rats. [In Persian]
21. Khodabandeh M, Peeri M, Azarbayjani MA, Matinhomaee H. Effect of resistance exercise and liposomal vitamin C on some factors of mitochondrial dynamics and biogenesis. Complementary Medicine Journal. 2021 Jun 10;11(1):82-97. [In Persian]
22. Shabani M, Choobineh S, Kordi MR, Afghan M. The effect of 8 weeks of high intensity interval training on the expression of PGC-1α and VEGF genes in myocardial muscle of male healthy rats. Journal of sport biosciences. 2016 Aug 22;8(2):169-76. [In Persian]