اثر همزمان تمرین هوازی و عصاره اتانولی پوست نارنج ایرانی بر بیان ژن PGC-1α بافت کبد رت‌های ماده چاق نژاد ویستار
محورهای موضوعی : فعالیت بدنی و تندرستی
امیر حسام سلماسی فرد
1
,
محمد علی آذربایجانی
2
*
,
فرهاد ریاضی راد
3
,
مقصود پیری
4
,
حسن متین همایی
5
1 - گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
2 - استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران، ایران
3 - گروه ایمونولوژی، انستیتو پاستور ایران، تهران، ایران
4 - گروه فیزیولوژی، واحد تهران مرکزی، دانشگاه آزاد اسلامی،تهران،ایران
5 - گروه فیزیولوژی ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: تمرین هوازی, پوست نارنج, PGC-1α, چاقی,
چکیده مقاله :
هدف: پژوهش حاضر به بررسی اثر همزمان تمرین هوازی و عصاره اتانولی پوست نارنج بر روی PGC-1α در بافت کبد رت‌های ماده ویستار چاق پرداخته است. روش: برای این پژوهش رت‌های نژاد ویستار به طور تصادفی در 5 گروه 6 تایی، کنترل سالم، چاق، چاق+تمرین هوازی، چاق+عصاره و چاق+تمرین هوازی+عصاره تقسیم شدند و پروتکل چاقی اعمال شد. تمرین هوازی بصورت 5 روز در هفته به مدت 30 دقیقه با شدت متوسط انجام شد. عصاره اتانولی پوست نارنج با دوز 60 میلی‌گرم به ازای هر کیلوگرم از وزن رت‌ها بصورت گاواژ به مدت 6 هفته صورت گرفت. 48 ساعت بعد اتمام پروتکل‌ها رت‌ها قربانی شدند و بافت کبد خارج و بلافاصله در دمای 80- منجمد شد. برای بیان ژن PGC-1α در بافت کبد از روش Real-Time PCR استفاده شد. برای تحلیل داده از آزمون t مستقل و تحلیل دو راهه واریانس ANOVA با نرم افزار GraphPad Prism نسخه 10 با سطح معناداری 0.05 =p انجام شد. یافته‌ها: نتایج نشان داد چاقی باعث کاهش معناداری در بیان ژن PGC-1α نسبت به گروه کنترل سالم شد(0.001 =p). تفاوت معناداری بین گروه تمرین هوازی، تمرین هوازی+عصاره اتانولی پوست نارنج و عصاره اتانولی پوست نارنج با گروه چاق و کنترل سالم در بیان PGC-1α وجود داشت(0.001 =p)، بیشترین بیان مربوط به گروه تمرین هوازی + عصاره اتانولی پوست نارنج بود. نتیجه‌گیری: بر اساس نتایج به دست آمده از این پژوهش، تمرین هوازی و عصاره اتانولی پوست نارنج به واسطه افزایش بیان ژن PGC-1α می‌تواند آسیب‌های هپاتوسیتی ناشی از تغذیه با غذای پرچرب و چاقی را کاهش دهد.
Objective: The present study investigated the simultaneous effect of aerobic training and bitter orange peel on PGC-1α in the liver tissue of obese female Wistar rats. Methods: For this study, Wistar rats were randomly divided into 5 groups of 6: healthy control, obese, obese+AT, obese+extract, and obese+AT+extract, and obesity protocol was applied. Aerobic training (AT) was done 5 days a week for 30 minutes with moderate intensity. Ethanol extract of bitter orange peel with a dose of 60 mg per kg of the rat's weight was administered by gavage for 6 weeks. The rats were sacrificed 48 hours after completing the protocols, and the liver tissue was removed and immediately frozen at -80. The real-time PCR method expressed the PGC-1α gene in liver tissue. For data analysis, independent t-tests and two-way analyses of variance ANOVA were performed using GraphPad Prism version 10 software with a significance level of p=0.05. Findings: The results showed that obesity caused a significant decrease in PGC-1α gene expression compared to the healthy control group (p=0.001). There was a significant difference in the expression of PGC-1α between the AT group, AT+bitter orange peel, and ethanol extract of orange peel with the obese group and healthy control (p=0.001); the highest expression was related to the group of AT+bitter orange peel ethanol extract. Conclusion: According to the research findings, aerobic training and the consumption of bitter orange can help reduce hepatocyte damage caused by a high-fat diet and obesity by enhancing the expression of the PGC-1α gene.
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