اثر محافظتی عصاره اتانولی خاکشیر (Descurainia sophia) بر میزان آنزیمهای آنتیاکسیدان در مسمومیت کبدی القاءشده توسط تتراکلریدکربن در موشهای صحرایی
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
محبوبه محلوجی
1
,
اکرم عیدی
2
,
سید پژمان مرتضوی
3
,
شهربانو عریان
4
1 - کارشناس ارشد فیزیولوژی جانوری، گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - کارشناس ارشد فیزیولوژی جانوری، گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - دانشیار گروه پاتولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
4 - استاد گروه زیست شناسی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران.
تاریخ دریافت : 1401/07/20
تاریخ پذیرش : 1401/12/01
تاریخ انتشار : 1402/02/01
کلید واژه:
مسمومیت کبدی,
تتراکلریدکربن,
خاکشیر,
حفاظت کبدی,
موش صحرایی.,
چکیده مقاله :
در مطالعات گوناگون اثرات ضدالتهابی و آنتی اکسیدانی گیاه خاکشیر اثبات شده است. هدف از مطالعه حاضر، ارزیابی اثرات محافظتی عصاره خاکشیر در آسیب کبدی ناشی از تتراکلریدکربن در موش های صحرایی بود. در این مطالعه تجربی، تعداد 60 سر موش صحرایی نر بالغ نژاد ویستار به طور تصادفی به 10 گروه 6تایی تقسیم شدند که شامل گروه های: کنترل سالم، کنترل مسموم (تزریق داخل صفاقی 5/0 میلی لیتر بر کیلوگرم وزن بدن تتراکلریدکربن)، تجربی سالم (گاواژ عصاره اتانولی خاکشیر با دوزهای 10، 50، 100 و 200 میلی گرم بر کیلوگرم وزن بدن)، تجربی مسموم (تزریق داخل صفاقی تتراکلریدکربن 5/0 میلی لیتر بر کیلوگرم وزن بدن و گاواژ عصاره اتانولی خاکشیر 10، 50، 100 و 200 میلی گرم بر کیلوگرم وزن بدن) بودند. در پایان 28 روز (مدت زمان آزمایش)، سطح آنزیم های آنتی اکسیدانی سوپراکسید دیسموتاز، کاتالاز و گلوتاتیون پراکسیداز و میزان مالوندیآلدئید در هموزنات کبدی مورد ارزیابی قرار گرفت. داده ها با استفاده از آزمون آماری تحلیل واریانس یک طرفه و آزمون تعقیبی توکی بررسی و ملاک اختلاف آماری معنی دار، 05/0 p< بود. تتراکلریدکربن باعث کاهش معنی دار میزان آنزیم های آنتی اکسیدانی و افزایش معنی دار میزان مالون دی آلدئید در بافت کبد گروه کنترل مسموم نسبت به گروه کنترل سالم شد (001/0p<). همچنین تیمار با عصاره خاکشیر بهصورت وابسته به دوز، باعث افزایش معنی دار سطح آنزیم های آنتی اکسیدانی و کاهش معنی دار میزان مالون دی آلدئید در بافت کبد گروه تجربی مسموم نسبت به گروه کنترل مسموم گردید (05/0p<). نتایج مطالعه نشان داد گیاه خاکشیر احتمالاً به واسطه ترکیبات فلاونوئیدی خود باعث حذف رادیکال های آزاد و کاهش استرس اکسیداتیو ناشی از تتراکلریدکربن در بافت کبد موش صحرایی میشود.
چکیده انگلیسی:
Various studies have shown the anti-inflammatory and anti-oxidant effects of Descorainia sophia. The purpose of this study was to evaluate the hepatoprotective ability of Descorainia sophia ethanolic extract in liver damage induced by carbon tetrachloride in male Wistar rats. In this experimental study, 60 male Wistar rats were randomly divided into 10 groups of 6 consisting of normal control, intoxified control (intraperitoneal injection of 0.5 ml/kg of carbon tetrachloride), normal experimental (Descorainia sophia ethanolic extract at doses of 10, 50, 100 and 200 mg/kg via intragastric gavage) and intoxified experimental (intraperitoneal injection of 0.5 ml/kg of carbon tetrachloride and Descorainia sophia ethanolic extract at doses of 10, 50, 100 and 200 mg/kg via intragastric gavage). After 28 days, the levels of antioxidant enzymes viz. superoxide dismutase, catalase, glutathione peroxidase and level of malondialdehyde in liver homogenate were evaluated. Data were analyzed using one-way ANOVA and the Tukey post hoc test with statistical significance defined as p<0.05. Our results showed that administration of carbon tetrachloride significantly decreased the levels of antioxidant enzymes and increased the level of malondialdehyde in the intoxified control group in comparison to normal control group (p<0.001). Also, the administration of Descorainia sophia ethanolic extract significantly increased the levels of antioxidant enzymes and decreased the level of malondialdehyde in the liver of intoxified experimental groups in comparison with the intoxified control group (p<0.05) in a dose dependent manner. The results of the study indicated that Descorainia sophia removes free radicals and reduces oxidative stress caused by carbon tetrachloride in hepatic tissue of rats probably due to its flavonoid compounds.
منابع و مأخذ:
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• Ahmad, A., Pillai, K.K., Najmi, A.K., Ahmad, S.J., Pal, S.N. and Balani, D.K. (2002). Evaluation of hepatoprotective potential of jigrine posttreatment against thioacetamide induced hepatic damage. Journal of Ethnopharmacology, 79(1):35-41.
• Ahmed, B., Alam, T., Varshney, M. and Khan, S.A. (2002). Hepatoprotective of two plants belonging to the Apiaceae and the Euphorbiaceae family. Journal of Ethnopharmacology, 79(3): 313-316.
• Akbartabar Toori, M., Joodi, B., Sadeghi, H., Sadeghi, H., Jafari, M., Talebianpoor, M.S., et al. (2015). Hepatoprotective activity of aerial parts of Otostegia persica against carbon tetrachloride-induced liver damage in rats. Avicenna Journal of Phytomedicine, 5(3): 238-246.
• Amat, N., Upur, H. and Blazeković, B. (2010). In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. Journal of Ethnopharmacology, 131(2): 478-484.
• Androli, T., Carpenter, C., Griggs, R. and Benjamin, I. (2007). Diseases of the Liver and Biliary System. In: Cecil's Essentials of Medicine. 7th ed., USA: WB Saunders Company, pp:23.
• Aquino, R., Morelli, S., Lauro, M.R., Abdo, S., Saija, A. and Tomaino, A. (2001). Phenolic constituents and antioxidant activity of an extract of Anthurium versicolor leaves. Journal of Natural Products, 64(8): 1019-1023.
• Barnes, J., Anderson, L.A. and Phillipson, J.D. (2007). Herbal Medicines. 3th ed., Pharmaceutical Press, pp: 176-213.
• Chen, J.W., Zhu, Z.Q., Hu, T.X. and Zhu, D.Y. (2002). Structure activity relationship of natural flavonoids in hydroxyl radical scavenging effects. Acta Pharmacologica Sinica, 23(7): 667-672.
• Cho, B.O., Ryu, H.W., So, Y., Jin, C.H., Baek, J.Y., Park, K.H., et al. (2013). Hepatoprotective effect of 2,3-dehydrosilybin on carbon tetrachloride induced liver injury in rats. Food Chemistry, 138(1): 107-115.
• Debnath, S., Ghosh, S. and Hazra, B. (2013). Inhibitory effect of Nymphaea pubescens Wild. Flower extract on carrageenan induced inflammation and CCl4 induced hepatotoxicity in rats. Food and Chemical Toxicology, 59(1): 485-491.
• Duke, J.A. (1985). Medicinal plants. Science, 229(4718): 1036-1038.
• Ginès, P., Cárdenas, A., Arroyo, V. and Rodés, J. (2004). Management of cirrhosis and ascites. New England Journal of Medicine, 350(16): 1646-1654.
• Guyton, A. and Hall, J.E. (2016). Textbook of Medical Physiology. 13th ed., Sepehri, H., Ghasemi, K., Rastgarfarajzadeh, A., editors. translators. Iran: Tehran Andisheh Rafee, pp: 1094-1095. [In Persian]
• Ha, B.J. and Lee, J.Y. (2003). The effect of chonderitin sulfate against CCl4 induced hepatotoxicity. Biological and Pharmaceutical Bulletin, 26(5): 622-626.
• Halliwell, B. (2012). Free radicals and antioxidants: updating a personal view. Nutrition Reviews, 70(5): 257-65.
• Hollman, P.C.H. (2001). Evidence for health benefits of plant phenols: local or systemic effects? Journal of the Science of Food and Agriculture, 81(9): 842-852.
• Jan, L. (1999). Pharmaceutical Botanic. 4th ed. Qoqnus Publication. pp: 235. [In Persian]
• Kepekçi, R.A., Polat, S., Çelik, A., Bayat, N. and Saygideger, S.D. (2013). Protective effect of Spirulina platensis enriched in phenolic compounds against hepatotoxicity induced by CCl4. Food Chemistry, 141(3): 1972-1979.
• Ledda-Columbano, G.M., Coni, P., Curto, M., Giacomini, L., Faa, G., Oliverio, S., et al. (1991). Induction of two different models of cell death, apoptosis and necrosis in rat liver after a single dose of thioacetamide. The American Journal of Pathology, 139(5): 1099-1109.
• Li, G.Y., Gao, H.Y., Huang, J., Lu, J., Gu, J.K. and Wang, J.H. (2014). Hepatoprotective effect of Cichorium intybus L., a traditional Uighur medicine, against carbon tetrachloride-induced hepatic fibrosis in rats. World Journal of Gastroenterology, 20(16): 4753-4760.
• Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4): 523-530.
• Mohamed, N.H. and Mahrous, A.E. (2009).Chemical constituents of Descurainia sophia L. and its biological activity. Records of Natural Products, 3(1): 58-67.
• Mokhtari, M., Shariati, M. and Khodaparast, L. (2008). Hepatoprotective effect of Mentha pulegium aquaethanolic leaf extract in rats. Journal of Sabzevar University of Medical of Sciences, 15(2): 73-78. [In Persian]
• Moshaie-Nezhad P., Iman M., Faed Maleki F. and Khamesipour A. (2018). Hepatoprotective effect of Descurainia sophia seed extract against paracetamol-induced oxidative stress and hepatic damage in mice. Journal of Herb Medical Pharmacology, 7(4): 267-272.
• Pradeepa, K., Krishna, V., Venkatesh, R., Santosh Kumar, S.R. and Rajesh Kashi, P.G. (2014). Antioxidant and prophylactic effects of Delonix elata L., stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats. BioMed Research International, 14.
• Rafiee, F., Heidari, R., Ashraf, H. and Rafiee, P. (2013). Protective effect of Berberis integerrima fruit extract on carbon-tetrachloride induced hepatotoxicity in rats. Journal of Fasa University of Medical Sciences, 3(3):179-187. [In Persian]
• Rajesh, K.P., Manjunatha, H., Krishna, V. and Swamy, K.B.E. (2013). Potential in vitro antioxidant and protective effects of Mesua ferrea Linn. bark extracts on induced oxidative damage. Industrial Crops and Products, 47: 186-198.
• Ranjbar, A., Ghaseminejhad, S., Takalu, H., Rahimi, F. and Abdollahi, M. (2007). Antioxidative stress potential of Cinnamon (Cinnamomun zelanicum) in operating room personnel: a before/after cross sectional clinical trial. International Journal of Pharmacology, 3(6):482-486.
• Shah, M.D., Gnanaraj, C., Emdadul Haque, A.T.M. and Iqbal, M. (2015). Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. Pharmaceutical Biology, 53(1): 31-39,
• Soni, B., Visavadiya, N.P. and Madamwar, D. (2008). Ameliorative action of cyanobacterial phycoerythrin on CCl4-induced toxicity in rats. Journal of Toxicology, 248(1): 59-65.
• Suzek, H., Celik, I., Dogan, A. and Yildirim, S. (2016). Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Journal of Pharmaceutical Biology,54(3): 451-457.
• Williams, G.M., Iatropoulos, M.J. and Whysner, J. (1999). Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food and Chemical Toxicology, 37(9-10): 1027-1038.
• Wojtaszek, P. (1997). Oxidative burst: an early plant response to pathogen infection. Biochemical Journal, 322(3): 681-692.
• Yacout, G.A., Elguindy, N.M. and El Azab, E.F. (2012). Hepatoprotective effect of basil (Ocimum basilicum L.) on CCl4-induced liver fibrosis in rats. African Journal of Biotechnology, 11(90): 15702-15711.
• Yao, L.H., Jiang, Y.M., Shi, J., Tomas-Barberan, F.A., Datta, N., Singanusong, R., et al. (2004). Flavonoids in food and their health benefits. Plant Foods for Human Nutrition, 59(3): 113-122.
• Zakim, D. and Boyer, T.D. (1990). Hepatology: A Textbook of Liver Disease. 2th ed., The University of Michigan: Saunders, Inc., pp: 750.
• Zargari, A. (1990). Medicinal Plants. 4th ed., Iran: Tehran University Press, pp: 28-42. [In Persian]
• Zhongfeng, X. (2007). Determination of trace elements in Descurainia sophia by FAAS. Journal of Anhui Agricultural Sciences, 35(33): 10575.
_||_Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S. and Rezaiee, A. (2004). Pesticides and oxidative stress: a review. Medical Science Monitor, 10(6): 141-147.
• Ahmad, A., Pillai, K.K., Najmi, A.K., Ahmad, S.J., Pal, S.N. and Balani, D.K. (2002). Evaluation of hepatoprotective potential of jigrine posttreatment against thioacetamide induced hepatic damage. Journal of Ethnopharmacology, 79(1):35-41.
• Ahmed, B., Alam, T., Varshney, M. and Khan, S.A. (2002). Hepatoprotective of two plants belonging to the Apiaceae and the Euphorbiaceae family. Journal of Ethnopharmacology, 79(3): 313-316.
• Akbartabar Toori, M., Joodi, B., Sadeghi, H., Sadeghi, H., Jafari, M., Talebianpoor, M.S., et al. (2015). Hepatoprotective activity of aerial parts of Otostegia persica against carbon tetrachloride-induced liver damage in rats. Avicenna Journal of Phytomedicine, 5(3): 238-246.
• Amat, N., Upur, H. and Blazeković, B. (2010). In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. Journal of Ethnopharmacology, 131(2): 478-484.
• Androli, T., Carpenter, C., Griggs, R. and Benjamin, I. (2007). Diseases of the Liver and Biliary System. In: Cecil's Essentials of Medicine. 7th ed., USA: WB Saunders Company, pp:23.
• Aquino, R., Morelli, S., Lauro, M.R., Abdo, S., Saija, A. and Tomaino, A. (2001). Phenolic constituents and antioxidant activity of an extract of Anthurium versicolor leaves. Journal of Natural Products, 64(8): 1019-1023.
• Barnes, J., Anderson, L.A. and Phillipson, J.D. (2007). Herbal Medicines. 3th ed., Pharmaceutical Press, pp: 176-213.
• Chen, J.W., Zhu, Z.Q., Hu, T.X. and Zhu, D.Y. (2002). Structure activity relationship of natural flavonoids in hydroxyl radical scavenging effects. Acta Pharmacologica Sinica, 23(7): 667-672.
• Cho, B.O., Ryu, H.W., So, Y., Jin, C.H., Baek, J.Y., Park, K.H., et al. (2013). Hepatoprotective effect of 2,3-dehydrosilybin on carbon tetrachloride induced liver injury in rats. Food Chemistry, 138(1): 107-115.
• Debnath, S., Ghosh, S. and Hazra, B. (2013). Inhibitory effect of Nymphaea pubescens Wild. Flower extract on carrageenan induced inflammation and CCl4 induced hepatotoxicity in rats. Food and Chemical Toxicology, 59(1): 485-491.
• Duke, J.A. (1985). Medicinal plants. Science, 229(4718): 1036-1038.
• Ginès, P., Cárdenas, A., Arroyo, V. and Rodés, J. (2004). Management of cirrhosis and ascites. New England Journal of Medicine, 350(16): 1646-1654.
• Guyton, A. and Hall, J.E. (2016). Textbook of Medical Physiology. 13th ed., Sepehri, H., Ghasemi, K., Rastgarfarajzadeh, A., editors. translators. Iran: Tehran Andisheh Rafee, pp: 1094-1095. [In Persian]
• Ha, B.J. and Lee, J.Y. (2003). The effect of chonderitin sulfate against CCl4 induced hepatotoxicity. Biological and Pharmaceutical Bulletin, 26(5): 622-626.
• Halliwell, B. (2012). Free radicals and antioxidants: updating a personal view. Nutrition Reviews, 70(5): 257-65.
• Hollman, P.C.H. (2001). Evidence for health benefits of plant phenols: local or systemic effects? Journal of the Science of Food and Agriculture, 81(9): 842-852.
• Jan, L. (1999). Pharmaceutical Botanic. 4th ed. Qoqnus Publication. pp: 235. [In Persian]
• Kepekçi, R.A., Polat, S., Çelik, A., Bayat, N. and Saygideger, S.D. (2013). Protective effect of Spirulina platensis enriched in phenolic compounds against hepatotoxicity induced by CCl4. Food Chemistry, 141(3): 1972-1979.
• Ledda-Columbano, G.M., Coni, P., Curto, M., Giacomini, L., Faa, G., Oliverio, S., et al. (1991). Induction of two different models of cell death, apoptosis and necrosis in rat liver after a single dose of thioacetamide. The American Journal of Pathology, 139(5): 1099-1109.
• Li, G.Y., Gao, H.Y., Huang, J., Lu, J., Gu, J.K. and Wang, J.H. (2014). Hepatoprotective effect of Cichorium intybus L., a traditional Uighur medicine, against carbon tetrachloride-induced hepatic fibrosis in rats. World Journal of Gastroenterology, 20(16): 4753-4760.
• Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4): 523-530.
• Mohamed, N.H. and Mahrous, A.E. (2009).Chemical constituents of Descurainia sophia L. and its biological activity. Records of Natural Products, 3(1): 58-67.
• Mokhtari, M., Shariati, M. and Khodaparast, L. (2008). Hepatoprotective effect of Mentha pulegium aquaethanolic leaf extract in rats. Journal of Sabzevar University of Medical of Sciences, 15(2): 73-78. [In Persian]
• Moshaie-Nezhad P., Iman M., Faed Maleki F. and Khamesipour A. (2018). Hepatoprotective effect of Descurainia sophia seed extract against paracetamol-induced oxidative stress and hepatic damage in mice. Journal of Herb Medical Pharmacology, 7(4): 267-272.
• Pradeepa, K., Krishna, V., Venkatesh, R., Santosh Kumar, S.R. and Rajesh Kashi, P.G. (2014). Antioxidant and prophylactic effects of Delonix elata L., stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats. BioMed Research International, 14.
• Rafiee, F., Heidari, R., Ashraf, H. and Rafiee, P. (2013). Protective effect of Berberis integerrima fruit extract on carbon-tetrachloride induced hepatotoxicity in rats. Journal of Fasa University of Medical Sciences, 3(3):179-187. [In Persian]
• Rajesh, K.P., Manjunatha, H., Krishna, V. and Swamy, K.B.E. (2013). Potential in vitro antioxidant and protective effects of Mesua ferrea Linn. bark extracts on induced oxidative damage. Industrial Crops and Products, 47: 186-198.
• Ranjbar, A., Ghaseminejhad, S., Takalu, H., Rahimi, F. and Abdollahi, M. (2007). Antioxidative stress potential of Cinnamon (Cinnamomun zelanicum) in operating room personnel: a before/after cross sectional clinical trial. International Journal of Pharmacology, 3(6):482-486.
• Shah, M.D., Gnanaraj, C., Emdadul Haque, A.T.M. and Iqbal, M. (2015). Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. Pharmaceutical Biology, 53(1): 31-39,
• Soni, B., Visavadiya, N.P. and Madamwar, D. (2008). Ameliorative action of cyanobacterial phycoerythrin on CCl4-induced toxicity in rats. Journal of Toxicology, 248(1): 59-65.
• Suzek, H., Celik, I., Dogan, A. and Yildirim, S. (2016). Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Journal of Pharmaceutical Biology,54(3): 451-457.
• Williams, G.M., Iatropoulos, M.J. and Whysner, J. (1999). Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food and Chemical Toxicology, 37(9-10): 1027-1038.
• Wojtaszek, P. (1997). Oxidative burst: an early plant response to pathogen infection. Biochemical Journal, 322(3): 681-692.
• Yacout, G.A., Elguindy, N.M. and El Azab, E.F. (2012). Hepatoprotective effect of basil (Ocimum basilicum L.) on CCl4-induced liver fibrosis in rats. African Journal of Biotechnology, 11(90): 15702-15711.
• Yao, L.H., Jiang, Y.M., Shi, J., Tomas-Barberan, F.A., Datta, N., Singanusong, R., et al. (2004). Flavonoids in food and their health benefits. Plant Foods for Human Nutrition, 59(3): 113-122.
• Zakim, D. and Boyer, T.D. (1990). Hepatology: A Textbook of Liver Disease. 2th ed., The University of Michigan: Saunders, Inc., pp: 750.
• Zargari, A. (1990). Medicinal Plants. 4th ed., Iran: Tehran University Press, pp: 28-42. [In Persian]
• Zhongfeng, X. (2007). Determination of trace elements in Descurainia sophia by FAAS. Journal of Anhui Agricultural Sciences, 35(33): 10575.