تاثیر تابش لیزر کم توان فراجمجمه ای بر اختلالات حافظه اجتنابی و برهم کنش اجتماعی ناشی از بی وزنی شبیه سازی شده در موش نر نژاد Balb/C
سید زانیار اطهری
1
(
گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.
)
جواد محمودی
2
(
مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی تبریز، تبریز، ایران
)
فرشته فرج دخت
3
(
گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.
)
کلید واژه: لیزرتراپی کم توان, بر هم کنش اجتماعی, بی¬وزنی, فضانوردی, موش,
چکیده مقاله :
تعاملات اجتماعی و اختلالات حافظه اجتنابی ازعوارض اصلی و مهم مغزی به دنبال بی‌وزنی در شرایط فضانوردی میباشد.لیزرکم‌توان فراجمجمه‌ای یک روش غیرتهاجمی است که دارای اثرات محافظت کننده مغزی و بهبوددهنده عملکرد شناختی با تاثیر بر شکل پذیری نورونی در مغز است. هدف از این مطالعه، ارزیابی اثر تابش لیزر کم‌توان فراجمجمه‌ای بر اختلالات حافظه اجتنابی و تعاملات اجتماعی ناشی از بی‌وزنی شبیه‌سازی‌شده در موش‌های نر نژادBalb/Cمیباشد. در این مطالعه تجربی، 24 سر موش نر نژادBalb/Cبه سه گروه مساوی شامل کنترل، بی‌وزنی القایی(HU)و همچنینHUبه همراه تیمار با لیزر(HU+PBM)تقسیم شدند. مدل بی‌وزنی با استفاده از تکنیک معلق سازی اندام‌های خلفی(Hindlimb Unloading)به مدت14روز اعمال گردید و در گروهHU+PBMهمزمان با القاء مدل، حیوانات با لیزر با طول موج810نانومتر تیمار شدند. سپس ارزیابی رفتاری با آزمون‌های حافظه اجتنابی و تعامل اجتماعی انجام شد. همچنین، میزان عامل نوروتروفیک مشتق از مغز(BDNF)به‌عنوان شاخصی از نوروپلاستیسیتی در بخش پره فرونتال مغز با روشELISAاندازه‌گیری شد. دادههای حاصله با آزمون آماری آنوا یکطرفه و تست تعقیبی توکی آنالیز شدند. یافته‌ها نشان داد که بی‌وزنی شبیه‌سازی‌شده موجب کاهش معنیدار در شاخص‌های برهم کنش اجتماعی، اختلال در حافظه اجتنابی و افت سطحBDNFشد(05/0p<). در مقابل، تیمار با لیزر کم‌توان در گروه HU+PBM به‌طور معنادار این شاخص‌ها را بهبود بخشید(05/0p<). نتایج این مطالعه نشان داد که لیزر کم‌توان احتمالا از طریق افزایشBDNF، می‌تواند به‌عنوان یک مداخله غیرتهاجمی مؤثردر مقابله با اختلالات تعاملات اجتماعی و حافظه اجتنابی ناشی از شرایط بی وزنی فضایی مطرح شود.
چکیده انگلیسی :
Social interaction impairments and avoidance memory deficits are among the key neurological complications associated with microgravity during spaceflight.Transcranial low-level laser therapy(tLLLT),as a non-invasive intervention,has been proposed as a potential strategy to counteract these deficits by modulating brain plasticity.This study aimed to evaluate the effects of tLLLT on social interaction and avoidance memory disorders induced by simulated microgravity in male Balb/C mice.In this experimental study,24male Balb/C mice were randomly divided into three equal groups:Control,Hindlimb Unloading(HU),and HU with laser treatment(HU+PBM).Simulated microgravity was induced using the hindlimb unloading technique for a duration of 14-days and in the HU+PBM group,animals were treated with a laser at a wavelength of 810 nm concurrently with the induction of the HU model.Behavioral assessments were performed using the Inhibitory Avoidance test and the Social Interaction test.Additionally, prefrontal levels of brain-derived neurotrophic factor(BDNF) were measured using the ELISA method as a biomarker of neuroplasticity.Data were analyzed using one-way ANOVA followed by Tukey's post hoc test.The results demonstrated that simulated microgravity significantly reduced sociability indices,impaired avoidance memory,and decreased BDNF levels(p<0.05).In contrast,laser treatment in the HU+PBM group significantly improved all of these parameters compared to the HU group(p<0.05).These findings suggest that tLLLT,likely through upregulation of BDNF,may serve as an effective non-invasive approach for mitigating social and cognitive deficits induced by spaceflight-like conditions.
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