تاثیر مکمل سازی رسوراترول بر برخی شاخص های اکسایشی، التهابی و آسیب سلولی ناشی از تمرین مقاومتی شدید در زنان غیرورزشکار
محورهای موضوعی : علوم ورزشی و سلامت
1 - گروه تربیت بدنی و علوم ورزشی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
کلید واژه: التهاب, فعالیت ورزشی مقاومتی, استرس اکسایشی, رسوراترول,
چکیده مقاله :
رژیم غذایی حاوی پلی فنول بالا می تواند شرایط آنتی اکسیدانی بدن را بهبود داده و آسیب اکسیداتیو ناشی از ورزش و ترشح سیتوکین های پیش التهابی را کاهش دهد. با توجه به این موضوع، هدف از تحقیق حاضر بررسی تأثیر مکمل رسوراترول بر برخی شاخص های اکسایشی، التهابی و آسیب سلولی ناشی از فعالیت مقاومتی شدید در زنان غیر ورزشکار بود. 24 زن غیر ورزشکار سالم (شاخص توده بدن20-25 کیلوگرم بر متر مربع، سن 20-30 سال) به صورت داوطلبانه در تحقیق حاضر شرکت کردند. این افراد به‌طور تصادفی به دو گروه دارونما و مکمل رسوراترول (12 نفر در هر گروه) تقسیم شدند. آزمودنی‌های دو گروه یک هفته مکمل یا دارونما (گروه مکمل روزانه 400 میلی گرم رسوراترول و گروه دارونما روزانه به همان مقدار به صورت کپسول اسانس) مصرف کردند و بعد از ان در یک جلسه فعالیت مقاومتی با 85 درصد یک تکرار بیشینه شرکت کردند. در 3 مرحله (قبل از مکمل یاری، قبل از تمرین مقاومتی و بلافاصله پس از تمرین مقاومتی) خون‌گیری به عمل آمد و سطوح مالون دی آلدئید، ظرفیت آنتی اکسیدانی تام، کراتین کیناز، پروتین واکنش c و اسید اوریک مورد آنالیز قرار گرفت. نتایج تحقیق حاضر نشان داد که 7روز مصرف رسوراترول، در گروه مکمل توانست باعث افزایش ظرفیت آنتی اکسیدانی تام و کاهش مالون دی آلدئید نسبت به قبل از دوره مکمل سازی شود (01/0 ≥ p). همچنین مصرف مکمل رسوارترول توانست مانع افزایش معنادار کراتین کیناز و کاهش معنادار ظرفیت آنتی اکسیدانی تام بعد از فعالیت مقاومتی گردد (05/0 > p). یافته‌های تحقیق حاضر نشان داد که مکمل رسوراترول از طریق کاهش مالون دی آلدئید و کراتین کیناز و افزایش ظرفیت آنتی اکسیدانی تام، اثرات محافظتی در برابر آسیب اکسیداتیو ناشی از ورزش مقاومتی شدید در زنان جوان دارد. این اثرات مفید احتمالاً به فعالیت‌های آنتی‌اکسیدانی ذاتی رسوراترول نسبت داده می‌شود. در تحقیق حاضر، محدودیت هایی مانند کم بودن تعداد آزمودنی ها و استفاده از دامنه سنی و جنسیت خاص، باعث کاهش توانایی تعمیم دادن نتایج می شود. از طرف دیگر عدم امکان کنترل دقیق رژیم غذایی آزمودنی ها یکی دیگر از محدودیت های این تحقیق است. پیشنهاد می شود که در تحقیقات آینده از گروه های بزرگ تر و یا در سنین متفاوت استفاده شود تا تاثیر مکمل رسوراترول در سایر افراد بررسی شود. همچنین از محدودیت های تحقیق عدم امکان بررسی سایر فاکتورهای استرس اکسیداتیو بود که پیشنهاد می شود در تحقیقات آتی به تاثیر این مکمل بر سایر متغیرهای فیزیولوژیکی پرداخته شود تا درک عمیق تری و کامل تری از این مکمل بر فاکتورهای استرس اکسیداتیو ناشی از ورزش به دست آید.
A diet high in polyphenols can improve the antioxidant status of the body and reduce the oxidative damage caused by exercise and the release of pro-inflammatory cytokines so the aim of this study was to investigate the effect of resveratrol supplementation on certain oxidative, inflammatory, and cellular damage markers resulting from high-intensity resistance exercise in non-athletic women. 24 healthy non-athletic women (body mass index 20-25 kg/m2, age 20-30 years) voluntarily participated in the present study. These people were randomly divided into two groups: placebo and resveratrol supplement (12 people in each group). The subjects of two groups took a supplement or a placebo for one week (supplement group 400 mg resveratrol daily and placebo group the same amount daily in the form of essential oil capsules) and then participated in a resistance activity session with 85% of a maximum repetition. In 3 stages (before Yari supplementation, before resistance training and immediately after resistance training) blood was taken and the levels of malondialdehyde, total antioxidant capacity, creatine kinase, c-reactive protein and uric acid were analyzed. The results of the present study showed that seven days of resveratrol consumption in the supplement group led to an increase in total antioxidant capacity and a decrease in malondialdehyde levels compared to pre-supplementation (p ≤ 0.01). Additionally, resveratrol supplementation was able to prevent a significant increase in creatine kinase and a significant decrease in total antioxidant capacity after the resistance exercise (p < 0.05). The findings of the present research showed that resveratrol supplementation has protective effects against oxidative damage caused by intense resistance exercise in young women by reducing malondialdehyde and creatine kinase and increasing total antioxidant capacity. These beneficial effects are probably attributed to the inherent antioxidant activities of resveratrol. In the present study, limitations such as the small number of subjects and the use of a specific age and gender range reduce the ability to generalize the results. On the other hand, the inability to precisely control the subjects' diet is another limitation of this study. It is suggested that future studies use larger groups or groups of different ages to examine the effects of resveratrol supplementation in other individuals. Another limitation of the study was the inability to examine other oxidative stress factors, and it is suggested that future studies examine the effects of this supplement on other physiological variables to gain a deeper and more complete understanding of this supplement on exercise-induced oxidative stress factors.
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