تاثیر تمرین تداومی با و بدون محدویت کالری در بیان ژن‌های آمیلوئید بتا و پروتئین‌های Tau در بافت مغز رت های نر پیر
محورهای موضوعی : نقش بیان ژن در سلامتی
حوریه کریمی
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مهتاب معظمی
2
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علی یعقوبی
3
1 - دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه ازاد اسلامی واحد بجنورد، بجنورد، ایران.
2 - گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران
3 - گروه تربیت بدنی، دانشگاه آزاد اسلامی واحد بجنورد، بجنورد، ایران.
کلید واژه: سالمندی, ژن تائو, ژن آمیلوئید بتا, تمرین تداومی, محدودیت کالری,
چکیده مقاله :
زمینه و هدف: بیماری آلزایمر به عنوان شایعترین بیماری تحلیل برندۀ عصبی وابسته به سن منجر به تشکیل پلاک‌های آمیلوئیدی خارج سلولی متشکل از رسوب پروتئین آمیلوئیدی بتا در خارج سلول و رشته‌های درهم تنیدهی داخل نرونی متشکل از پروتئین‌های تائو می باشد. مواد و روشها: تعداد 36 سر رت نر پیر با توجه به معیارهای ورود (غذای یکسان، همگن بودن از نظر سن و وزن و شرایط نگهداری یکسان) به طور تصادفی به چهار گروه کنترل، تمرین تداومی، گروه تمرین تداومی با محدودیت وگروه بدون محدودیت کالری تقسیم شدند. تمامی رت ها هشت هفته به¬صورت 5 جلسه (6 هفته، در مجموع 30 جلسه) با دو هفته آماده سازی 60 دقیقه ای شرکت داده شدند. محدودیت کالری بعد از بررسی تغذیه رت‌ها به مدت دو هفته، ابتدا کاهش 30 درصد و سپس 20 درصد در غذای مصرفی اعمال شد. اندازه¬گیری قد و وزن و بافت¬برداری از مغز 24 ساعت پس از آخرین تمرین و با 12 ساعت ناشتایی انجام گرفت. نتایج: نتایج نشان داد که تفاوت معنی داری بین 4 گروه مورد بررسی در بیان و ژن آمیلوئید بتا در بافت مغز رت‌های نر پیر وجود ندارد(05/0<p). نتیجه گیری: با توجه به نتایج حاصل از مطالعه به نظر می رسد، تمرین تداومی با و بدون محدودیت کالری نتوانسته است موجب تغییر معنی دار در هیچ یک از گروههای تمرینی بر بیان ژن تائو و ژن آمیلوئید بتا در بافت مغز رت‌های نر سالمند با نگاه پیشگیری از آلزایمرشود.
Background and Purpose: Alzheimer's disease, as the most common age-related neurodegenerative disease that Amyloid plaques and interneuron filamentous coils are the two main symptoms of Alzheimer's disease, which are usually associated with amyloid angiopathy in the brain. Materials and methods: 36 old male mice were randomly divided into four control groups, continuous training group and continuous training group with restriction and calorie restriction group according to the entry criteria (same food, homogeneity in terms of age and weight, and same maintenance conditions). Mice participated in 5 sessions (6 weeks, a total of 30 sessions) or 4 sessions per week (8 weeks, a total of 28 sessions) of 60 minutes for eight weeks. Calorie restriction after examining the nutrition of mice for two weeks, between 25 and 30% of the daily calorie consumption of mice is reduced, and calorie restriction is applied in this way. The measurement of blood indices and body composition was done before and after the implementation of the training protocol. Data analysis was performed using the U-Man-Whitney test at a significant level. Results: Our results showed that there is no significant difference between the 4 investigated groups in the expression of tau gene and amyloid beta gene in old male mice with Alzheimer's disease. Conclusion: According to the factors obtained from the study, it seems that continuous training with and without caloric restriction has not been able to change the expression of tau gene and amyloid beta gene in old male rats.
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