تکثیر درون‌شیشه‌ای موثر ارکید فالانوپسیس رقم Karen Rockwell
محورهای موضوعی : مجله گیاهان زینتی
ابوالفضل ولی زاده
1
(گروه فضای سبز، واحد نور، دانشگاه آزاد اسلامی، نور، ایران)
جلال محمودی
2
(گروه فضای سبز، واحد نور، دانشگاه آزاد اسلامی، نور، ایران)
محسن محمدی
3
(گروه باغبانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران)
بهزاد کاویانی
4
(گروه باغبانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران)
کلید واژه: زغال فعال, ارکیداسه, تنظیم‌کننده‌های رشد گیاهی, اجسام شبه‌پروتوکورم, کشت بافت,
چکیده مقاله :
فالانوپسیس یک جنس ارکید با ارزش اقتصادی بالا در گلکاری جهان است که به عنوان یک گیاه گلدانی و گل شاخه‌بریده استفاده می‌شود. تنوع ژنتیکی بالا و عدم یکنواختی رویشی و تکثیر تولیدمثلی، تولید این ارکید را از نظر اقتصادی غیر جالب می‌کند. تکثیر درون‌شیشه‌ای تنها روش امکان‌پذیر در مقیاس بزرگ برای تکثیر فالانوپسیس است. هدف از مطالعه حاضر، بررسی اثر انواع و غلظت‌های -ɑنفتالن استیک اسید (NAA) و 6-بنزیل‌آدنین (BA) (هر دو در غلظت‌های ۰، 0/5، ۱، 1/5 و ۳ میلی‌گرم در لیتر، به‌صورت جداگانه یا ترکیبی)، در قالب طرح کاملا تصادفی در تکثیر درون‌شیشه‌ای فالانوپسیس بود. زغال فعال (AC) (۰، 0/5 و ۱ میلی‌گرم در لیتر) برای جلوگیری از قهوه‌ای‌شدن محیط و بافت‌ها به محیط اضافه شد. موراشیگ و اسکوگ (MS)و پروتوکورم به‌ترتیب به‌عنوان محیط کشت و ریزنمونه استفاده شدند. نتایج نشان داد که بیشترین تعداد برگ در محیط غنی‌شده با ۱ میلی‌گرم در لیتر NAA همراه با 5/1 میلی‌گرم در لیتر BA و ۱ میلی‌گرم در لیتر AC به‌دست آمد. تیمار حاوی ۵/۱ میلی‌گرم در لیتر NAA همراه با 0/5 میلی‌گرم در لیتر BA و ۱ میلی‌گرم در لیتر AC بیشترین تعداد ریشه را القا کرد. گیاهچه‌های کامل تولیدشده در شرایط درون‌شیشه‌ای به گلدان‌های حاوی مخلوطی از LECA (دانه‌های خاک رس منبسط‌شده سبک)، پیت ماس، کوکوپیت، خاک زغال چوب، کوکو چیپس و پرلیت منتقل شدند و در شرایط گلخانه‌ای با درصد بقای ۱۰۰ درصد سازگار شدند.
Phalaenopsis is an orchid genus of high economic value in world floriculture used as a pot plant and cut flowers. High genetic variation and the lack of uniformity in vegetative and reproductive propagation make the production of this orchid economically uninteresting. In vitro proliferation is the only large-scale feasible method for Phalaenopsis propagation. The purpose of the present study was to evaluate the effect of types and concentrations of ɑ-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) (both at the concentrations of 0.0, 0.5, 1.0, 1.5 and 3.0 mg l−1, individually or in combination), as a completely randomized design, on the in vitro propagation of Phalaenopsis schilleriana ‘Karen Rockwell’. Activated charcoal (AC; 0.0, 0.5 and 1.0 mg l−1) was added to the media for prevention of the browning of the media and tissues. Murashige and Skoog (MS), and protocorm were used as culture medium and explant, respectively. The results showed that the highest leaf number was obtained in medium enriched with 1.0 mg l−1 NAA together with 1.5 mg l−1 BA along with 1.0 mg l−1 AC. The treatment containing 1.5 mg l−1 NAA together with 0.5 mg l−1 BA along with 1.0 mg l−1 AC induced the highest number of roots. Fully in vitro-produced plantlets were transferred to pots containing a mixture of LECA (Light Expanded Clay Aggregate), peat moss, coco peat, charcoal soil, coco chips and perlite, and acclimatized in greenhouse conditions with 100% survival rate.
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