In Vitro Propagation is an Optimal Method for the Production of Orchid Phalaenopsis schilleriana ‘Karen Rockwell’

Subject Areas : Journal of Ornamental Plants

Abolfazl Valizadeh ¹ (1Department of Green Space, Nour Branch, Islamic Azad University, Nour, Iran)
Jalal Mahmoudi ² (Department of Green Space, Nour Branch, Islamic Azad University, Nour, Iran)
Mohsen Mohammadi ³ (Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)
Behzad Kaviani ⁴ (Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran)

Received: 2024-05-22 Accepted : 2024-07-17 Published : 2024-09-03

Keywords: Activated charcoal, Orchidaceae, Plant growth regulators, Protocorm-like bodies, Tissue culture,

Abstract :

Phalaenopsis is an orchid genus of high economic value in world floriculture used as a pot plant and cut flowers. High genetic variation and the lack of uniformity in vegetative and reproductive propagation make the production of this orchid economically uninteresting. In vitro proliferation is the only large-scale feasible method for Phalaenopsis propagation. The purpose of the present study was to evaluate the effect of types and concentrations of ɑ-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) (both at the concentrations of 0.0, 0.5, 1.0, 1.5 and 3.0 mg l⁻¹, individually or in combination), as a completely randomized design, on the in vitro propagation of Phalaenopsis schilleriana ‘Karen Rockwell’. Activated charcoal (AC; 0.0, 0.5 and 1.0 mg l⁻¹) was added to the media for prevention of the browning of the media and tissues. Murashige and Skoog (MS), and protocorm were used as culture medium and explant, respectively. The results showed that the highest leaf number was obtained in medium enriched with 1.0 mg l⁻¹ NAA together with 1.5 mg l⁻¹ BA along with 1.0 mg l⁻¹ AC. The treatment containing 1.5 mg l⁻¹ NAA together with 0.5 mg l⁻¹ BA along with 1.0 mg l⁻¹ AC induced the highest number of roots. Fully in vitro-produced plantlets were transferred to pots containing a mixture of LECA (Light Expanded Clay Aggregate), peat moss, coco peat, charcoal soil, coco chips and perlite, and acclimatized in greenhouse conditions with 100% survival rate.

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